ROR1 (NM_005012) Human Tagged ORF Clone

CAT#: RC214967

ROR1 (Myc-DDK-tagged)-Human receptor tyrosine kinase-like orphan receptor 1 (ROR1), transcript variant 1

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY ROR1 (Cat# RC214967, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ROR1(Cat# TA806826). Positive lysates LY401558 (100ug) and LC401558 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY401558) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC214967 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified ROR1 protein (Cat# TP314967). The protein was produced from HEK293T cells transfected with ROR1 cDNA clone (Cat# RC214967) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ROR1
• Synonyms: dJ537F10.1; NTRKR1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214967 representing NM_005012
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCACCGGCCGCGCCGCCGCGGGACGCGCCCGCCGCTCCTGGCGCTGCTGGCCGCGCTGCTGCTGGCCG
  CACGCGGGGCTGCTGCCCAAGAAACAGAGCTGTCAGTCAGTGCTGAATTAGTGCCTACCTCATCATGGAA
  CATCTCAAGTGAACTCAACAAAGATTCTTACCTGACCCTTGATGAACCAATGAATAACATCACCACGTCT
  CTGGGCCAGACAGCAGAACTGCACTGCAAAGTCTCTGGGAATCCACCTCCCACCATCCGCTGGTTCAAAA
  ATGATGCTCCTGTGGTCCAGGAGCCCCGGAGGCTCTCCTTTCGGTCCACCATCTATGGCTCTCGGCTGCG
  GATTAGAAACCTCGACACCACAGACACAGGCTACTTCCAGTGCGTGGCAACAAACGGCAAGGAGGTGGTT
  TCTTCCACTGGAGTCTTGTTTGTCAAGTTTGGCCCCCCTCCCACTGCAAGTCCAGGATACTCAGATGAGT
  ATGAAGAAGATGGATTCTGTCAGCCATACAGAGGGATTGCATGTGCAAGATTTATTGGCAACCGCACCGT
  CTATATGGAGTCTTTGCACATGCAAGGGGAAATAGAAAATCAGATCACAGCTGCCTTCACTATGATTGGC
  ACTTCCAGTCACTTATCTGATAAGTGTTCTCAGTTCGCCATTCCTTCCCTGTGCCACTATGCCTTCCCGT
  ACTGCGATGAAACTTCATCCGTCCCAAAGCCCCGTGACTTGTGTCGCGATGAATGTGAAATCCTGGAGAA
  TGTCCTGTGTCAAACAGAGTACATTTTTGCAAGATCAAATCCCATGATTCTGATGAGGCTGAAACTGCCA
  AACTGTGAAGATCTCCCCCAGCCAGAGAGCCCAGAAGCTGCGAACTGTATCCGGATTGGAATTCCCATGG
  CAGATCCTATAAATAAAAATCACAAGTGTTATAACAGCACAGGTGTGGACTACCGGGGGACCGTCAGTGT
  GACCAAATCAGGGCGCCAGTGCCAGCCATGGAATTCCCAGTATCCCCACACACACACTTTCACCGCCCTT
  CGTTTCCCAGAGCTGAATGGAGGCCATTCCTACTGCCGCAACCCAGGGAATCAAAAGGAAGCTCCCTGGT
  GCTTCACCTTGGATGAAAACTTTAAGTCTGATCTGTGTGACATCCCAGCTTGCGATTCAAAGGATTCCAA
  GGAGAAGAATAAAATGGAAATCCTGTACATACTAGTGCCAAGTGTGGCCATTCCCCTGGCCATTGCTTTA
  CTCTTCTTCTTCATTTGCGTCTGTCGGAATAACCAGAAGTCATCGTCGGCACCAGTCCAGAGGCAACCAA
  AACACGTCAGAGGTCAAAATGTAGAGATGTCAATGCTGAATGCATATAAACCCAAGAGCAAGGCTAAAGA
  GCTACCTCTTTCTGCTGTACGCTTTATGGAAGAATTGGGTGAGTGTGCCTTTGGAAAAATCTATAAAGGC
  CATCTCTATCTCCCAGGCATGGACCATGCTCAGCTGGTTGCTATCAAGACCTTGAAAGACTATAACAACC
  CCCAGCAATGGATGGAATTTCAACAAGAAGCCTCCCTAATGGCAGAACTGCACCACCCCAATATTGTCTG
  CCTTCTAGGTGCCGTCACTCAGGAACAACCTGTGTGCATGCTTTTTGAGTATATTAATCAGGGGGATCTC
  CATGAGTTCCTCATCATGAGATCCCCACACTCTGATGTTGGCTGCAGCAGTGATGAAGATGGGACTGTGA
  AATCCAGCCTGGACCACGGAGATTTTCTGCACATTGCAATTCAGATTGCAGCTGGCATGGAATACCTGTC
  TAGTCACTTCTTTGTCCACAAGGACCTTGCAGCTCGCAATATTTTAATCGGAGAGCAACTTCATGTAAAG
  ATTTCAGACTTGGGGCTTTCCAGAGAAATTTACTCCGCTGATTACTACAGGGTCCAGAGTAAGTCCTTGC
  TGCCCATTCGCTGGATGCCCCCTGAAGCCATCATGTATGGCAAATTCTCTTCTGATTCAGATATCTGGTC
  CTTTGGGGTTGTCTTGTGGGAGATTTTCAGTTTTGGACTCCAGCCATATTATGGATTCAGTAACCAGGAA
  GTGATTGAGATGGTGAGAAAACGGCAGCTCTTACCATGCTCTGAAGACTGCCCACCCAGAATGTACAGCC
  TCATGACAGAGTGCTGGAATGAGATTCCTTCTAGGAGACCAAGATTTAAAGATATTCACGTCCGGCTTCG
  GTCCTGGGAGGGACTCTCAAGTCACACAAGCTCTACTACTCCTTCAGGGGGAAATGCCACCACACAGACA
  ACCTCCCTCAGTGCCAGCCCAGTGAGTAATCTCAGTAACCCCAGATATCCTAATTACATGTTCCCGAGCC
  AGGGTATTACACCACAGGGCCAGATTGCTGGTTTCATTGGCCCGCCAATACCTCAGAACCAGCGATTCAT
  TCCCATCAATGGATACCCAATACCTCCTGGATATGCAGCGTTTCCAGCTGCCCACTACCAGCCAACAGGT
  CCTCCCAGAGTGATTCAGCACTGCCCACCTCCCAAGAGTCGGTCCCCAAGCAGTGCCAGTGGGTCGACTA
  GCACTGGCCATGTGACTAGCTTGCCCTCATCAGGATCCAATCAGGAAGCAAATATTCCTTTACTACCACA
  CATGTCAATTCCAAATCATCCTGGTGGAATGGGTATCACCGTTTTTGGCAACAAATCTCAAAAACCCTAC
  AAAATTGACTCAAAGCAAGCATCTTTACTAGGAGACGCCAATATTCATGGACACACCGAATCTATGATTT
  CTGCAGAACTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214967 representing NM_005012
  Red=Cloning site Green=Tags(s)
  
  MHRPRRRGTRPPLLALLAALLLAARGAAAQETELSVSAELVPTSSWNISSELNKDSYLTLDEPMNNITTS
  LGQTAELHCKVSGNPPPTIRWFKNDAPVVQEPRRLSFRSTIYGSRLRIRNLDTTDTGYFQCVATNGKEVV
  SSTGVLFVKFGPPPTASPGYSDEYEEDGFCQPYRGIACARFIGNRTVYMESLHMQGEIENQITAAFTMIG
  TSSHLSDKCSQFAIPSLCHYAFPYCDETSSVPKPRDLCRDECEILENVLCQTEYIFARSNPMILMRLKLP
  NCEDLPQPESPEAANCIRIGIPMADPINKNHKCYNSTGVDYRGTVSVTKSGRQCQPWNSQYPHTHTFTAL
  RFPELNGGHSYCRNPGNQKEAPWCFTLDENFKSDLCDIPACDSKDSKEKNKMEILYILVPSVAIPLAIAL
  LFFFICVCRNNQKSSSAPVQRQPKHVRGQNVEMSMLNAYKPKSKAKELPLSAVRFMEELGECAFGKIYKG
  HLYLPGMDHAQLVAIKTLKDYNNPQQWMEFQQEASLMAELHHPNIVCLLGAVTQEQPVCMLFEYINQGDL
  HEFLIMRSPHSDVGCSSDEDGTVKSSLDHGDFLHIAIQIAAGMEYLSSHFFVHKDLAARNILIGEQLHVK
  ISDLGLSREIYSADYYRVQSKSLLPIRWMPPEAIMYGKFSSDSDIWSFGVVLWEIFSFGLQPYYGFSNQE
  VIEMVRKRQLLPCSEDCPPRMYSLMTECWNEIPSRRPRFKDIHVRLRSWEGLSSHTSSTTPSGGNATTQT
  TSLSASPVSNLSNPRYPNYMFPSQGITPQGQIAGFIGPPIPQNQRFIPINGYPIPPGYAAFPAAHYQPTG
  PPRVIQHCPPPKSRSPSSASGSTSTGHVTSLPSSGSNQEANIPLLPHMSIPNHPGGMGITVFGNKSQKPY
  KIDSKQASLLGDANIHGHTESMISAEL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005012
• ORF Size: 2811 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005012.4
• RefSeq Size: 3358 bp
• RefSeq ORF: 2814 bp
• Locus ID: 4919
• UniProt ID: Q01973
• Cytogenetics: 1p31.3
• Domains: KR, FRI, pkinase, TyrKc, S_TKc, ig, IGc2, IG
• Protein Families: Druggable Genome, Protein Kinase, Transmembrane
• MW: 104.1 kDa
• Gene Summary: This gene encodes a receptor tyrosine kinase-like orphan receptor that modulates neurite growth in the central nervous system. The encoded protein is a glycosylated type I membrane protein that belongs to the ROR subfamily of cell surface receptors. It is a pseudokinase that lacks catalytic activity and may interact with the non-canonical Wnt signalling pathway. This gene is highly expressed during early embryonic development but expressed at very low levels in adult tissues. Increased expression of this gene is associated with B-cell chronic lymphocytic leukaemia. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2012]