XPO6 (NM_015171) Human Tagged ORF Clone

CAT#: RC214839

XPO6 (Myc-DDK-tagged)-Human exportin 6 (XPO6)

Product Images

Circular map for RC214839

• 

  Western blot validation of overexpression lysate (Cat# LY414762) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC214839 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified XPO6 protein (Cat# TP314839). The protein was produced from HEK293T cells transfected with XPO6 cDNA clone (Cat# RC214839) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: XPO6
• Synonyms: EXP6; RANBP20
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214839 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCATCTGAAGAAGCCTCTCTCAGGGCATTGGAAAGTCTGATGACAGAATTTTTTCACGATTGTACAA
  CCAATGAAAGAAAACGTGAGATAGAGGAGCTTCTTAATAACTTTGCCCAGCAAATAGGAGCCTGGAGATT
  CTGCCTGTACTTTCTCTCCAGCACTAGGAATGACTATGTAATGATGTACAGTTTAACAGTTTTTGAGAAT
  CTGATCAATAAAATGTGGCTTGGGGTCCCATCTCAGGATAAGATGGAAATCCGTAGCTGTCTGCCCAAAC
  TCCTTTTGGCTCACCATAAAACCTTACCTTACTTTATCCGGAACAAGCTCTGCAAAGTTATTGTTGATAT
  TGGACGTCAGGATTGGCCCATGTTCTACCACGACTTTTTTACTAACATTTTACAGTTGATCCAGTCCCCT
  GTGACAACCCCCCTTGGGCTGATCATGTTGAAGACAACTTCAGAAGAGCTGGCTTGTCCCCGTGAGGACC
  TCAGTGTGGCTCGGAAGGAGGAGTTGCGGAAGCTGCTACTGGACCAGGTGCAGACAGTGCTTGGGCTACT
  GACAGGTATCTTGGAGACTGTCTGGGACAAACACAGTGTTACTGCTGCCACTCCACCACCATCCCCGACC
  TCAGGAGAAAGTGGTGACTTACTGAGTAACCTGTTGCAGAGTCCCAGTTCAGCCAAACTGTTGAATCAGC
  CAATTCCCATCCTTGATGTGGAGAGTGAGTATATCTGTTCCCTGGCTTTGGAGTGCCTGGCCCATCTCTT
  CAGTTGGATTCCTCTGTCTGCCAGCATCACCCCATCCCTCCTTACCACCATCTTCCACTTTGCACGATTT
  GGCTGTGACATCCGGGCCAGAAAGATGGCGTCAGTTAACGGCAGCAGCCAGAACTGTGTCTCGGGTCAGG
  AGCGCGGCCGGCTGGGGGTCCTGGCCATGTCCTGCATCAATGAACTCATGTCCAAGAACTGTGTGCCTAT
  GGAATTTGAGGAGTATTTACTGCGTATGTTCCAGCAGACTTTCTACCTCCTGCAGAAAATCACCAAGGAT
  AACAATGCCCACACAGTGAAGAGCAGGCTAGAAGAGCTCGATGAGAGCTATATCGAGAAGTTTACTGACT
  TTCTTCGGCTCTTTGTGAGTGTTCACCTAAGAAGAATCGAGTCTTATTCCCAGTTCCCTGTGGTGGAGTT
  TTTGACACTTTTGTTCAAGTACACATTTCATCAGCCTACTCATGAAGGTTACTTCTCTTGTTTGGATATC
  TGGACGCTGTTTTTGGACTATCTGACAAGTAAAATTAAAAGTCGTCTTGGAGACAAGGAAGCAGTTCTCA
  ACAGGTACGAAGATGCCCTGGTGCTCCTGCTCACAGAGGTGTTGAATCGAATCCAGTTCAGATACAACCA
  AGCCCAGCTGGAGGAGTTGGATGATGAGACTCTGGATGACGATCAGCAGACGGAGTGGCAGCGGTACTTA
  CGGCAGAGCTTGGAGGTGGTGGCCAAAGTGATGGAGCTCCTGCCCACGCACGCCTTCTCCACACTGTTCC
  CTGTTCTTCAGGACAATTTAGAAGTTTATTTGGGATTACAACAGTTTATAGTCACTTCAGGGTCAGGACA
  CAGGTTGAACATCACGGCGGAGAACGACTGCCGGCGGCTGCACTGCTCCCTGAGAGACTTGAGCTCCCTG
  CTGCAGGCCGTGGGCCGCCTGGCCGAGTACTTTATCGGGGATGTGTTTGCTGCACGGTTCAATGATGCCC
  TCACAGTCGTGGAAAGGTTGGTCAAAGTCACTCTGTACGGATCTCAGATAAAATTGTACAACATTGAAAC
  TGCTGTGCCATCAGTATTGAAACCTGACCTCATTGATGTGCATGCTCAGTCCCTGGCTGCGCTGCAGGCT
  TACTCTCACTGGTTAGCACAGTATTGCAGTGAAGTTCACCGGCAGAACACGCAGCAGTTCGTGACACTCA
  TCTCTACTACCATGGATGCAATCACACCTCTAATCAGCACCAAGGTCCAAGACAAGCTGCTGCTATCTGC
  GTGCCACTTACTGGTCTCACTGGCCACCACCGTGCGGCCCGTCTTTCTGATCAGCATCCCTGCAGTGCAG
  AAAGTATTCAACAGAATCACTGATGCCTCTGCCCTGCGACTTGTCGATAAGGCCCAGGTGTTGGTGTGCC
  GAGCCCTCTCTAACATCTTGCTGCTTCCGTGGCCAAACCTTCCAGAGAATGAGCAGCAGTGGCCCGTGCG
  CTCCATCAACCATGCCAGCCTCATCTCTGCACTCTCCCGGGACTATCGCAACCTGAAGCCCAGTGCTGTT
  GCCCCACAGAGAAAGATGCCACTGGATGACACCAAACTGATTATCCACCAGACACTCAGCGTCTTAGAAG
  ATATTGTGGAGAATATCTCGGGGGAGTCCACCAAGTCTCGACAGATTTGCTACCAGTCGCTGCAGGAATC
  TGTTCAGGTCTCCCTGGCCCTCTTTCCAGCTTTTATCCATCAGTCAGATGTGACTGATGAGATGCTGAGC
  TTCTTCCTCACTCTGTTTCGAGGCCTTAGAGTACAGATGGGTGTGCCTTTCACTGAGCAAATCATACAGA
  CTTTCCTCAACATGTTTACCAGAGAGCAGTTAGCCGAGAGCATCCTCCACGAGGGCAGCACAGGCTGCCG
  GGTGGTGGAGAAGTTTCTGAAGATCCTGCAGGTGGTGGTCCAGGAGCCAGGCCAGGTGTTCAAGCCCTTC
  CTCCCCAGCATCATCGCCCTGTGCATGGAGCAAGTGTATCCCATCATTGCCGAGCGTCCCTCCCCTGATG
  TGAAGGCCGAGCTGTTTGAGCTCCTTTTCCGGACGCTCCATCACAACTGGAGGTACTTCTTCAAGTCCAC
  CGTGCTGGCCAGTGTCCAGAGGGGGATCGCTGAGGAGCAGATGGAGAATGAGCCCCAGTTCAGTGCCATC
  ATGCAGGCTTTCGGACAGTCCTTTCTCCAGCCCGACATCCACCTTTTTAAACAAAATCTCTTCTACTTGG
  AGACTCTCAACACCAAGCAGAAGCTGTACCACAAGAAGATCTTCCGGACTGCCATGCTGTTCCAGTTTGT
  GAACGTGCTGCTCCAGGTCCTGGTCCACAAGTCCCATGATCTTCTGCAGGAGGAGATTGGCATCGCCATC
  TACAACATGGCCTCAGTCGACTTTGATGGCTTCTTTGCCGCCTTCCTCCCAGAGTTCCTGACCAGCTGTG
  ATGGTGTGGATGCCAACCAGAAAAGTGTGCTGGGGCGGAATTTCAAGATGGATCGGGACCTGCCCTCATT
  CACCCAGAATGTGCACAGGCTGGTCAACGACCTGCGCTACTACAGACTCTGCAACGACAGCCTGCCCCCT
  GGCACTGTGAAGCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214839 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MASEEASLRALESLMTEFFHDCTTNERKREIEELLNNFAQQIGAWRFCLYFLSSTRNDYVMMYSLTVFEN
  LINKMWLGVPSQDKMEIRSCLPKLLLAHHKTLPYFIRNKLCKVIVDIGRQDWPMFYHDFFTNILQLIQSP
  VTTPLGLIMLKTTSEELACPREDLSVARKEELRKLLLDQVQTVLGLLTGILETVWDKHSVTAATPPPSPT
  SGESGDLLSNLLQSPSSAKLLNQPIPILDVESEYICSLALECLAHLFSWIPLSASITPSLLTTIFHFARF
  GCDIRARKMASVNGSSQNCVSGQERGRLGVLAMSCINELMSKNCVPMEFEEYLLRMFQQTFYLLQKITKD
  NNAHTVKSRLEELDESYIEKFTDFLRLFVSVHLRRIESYSQFPVVEFLTLLFKYTFHQPTHEGYFSCLDI
  WTLFLDYLTSKIKSRLGDKEAVLNRYEDALVLLLTEVLNRIQFRYNQAQLEELDDETLDDDQQTEWQRYL
  RQSLEVVAKVMELLPTHAFSTLFPVLQDNLEVYLGLQQFIVTSGSGHRLNITAENDCRRLHCSLRDLSSL
  LQAVGRLAEYFIGDVFAARFNDALTVVERLVKVTLYGSQIKLYNIETAVPSVLKPDLIDVHAQSLAALQA
  YSHWLAQYCSEVHRQNTQQFVTLISTTMDAITPLISTKVQDKLLLSACHLLVSLATTVRPVFLISIPAVQ
  KVFNRITDASALRLVDKAQVLVCRALSNILLLPWPNLPENEQQWPVRSINHASLISALSRDYRNLKPSAV
  APQRKMPLDDTKLIIHQTLSVLEDIVENISGESTKSRQICYQSLQESVQVSLALFPAFIHQSDVTDEMLS
  FFLTLFRGLRVQMGVPFTEQIIQTFLNMFTREQLAESILHEGSTGCRVVEKFLKILQVVVQEPGQVFKPF
  LPSIIALCMEQVYPIIAERPSPDVKAELFELLFRTLHHNWRYFFKSTVLASVQRGIAEEQMENEPQFSAI
  MQAFGQSFLQPDIHLFKQNLFYLETLNTKQKLYHKKIFRTAMLFQFVNVLLQVLVHKSHDLLQEEIGIAI
  YNMASVDFDGFFAAFLPEFLTSCDGVDANQKSVLGRNFKMDRDLPSFTQNVHRLVNDLRYYRLCNDSLPP
  GTVKL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015171
• ORF Size: 3375 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015171.4
• RefSeq Size: 4504 bp
• RefSeq ORF: 3378 bp
• Locus ID: 23214
• UniProt ID: Q96QU8
• Cytogenetics: 16p12.1
• MW: 128.9 kDa
• Gene Summary: The protein encoded by this gene is a member of the importin-beta family. Members of this family are regulated by the GTPase Ran to mediate transport of cargo across the nuclear envelope. This protein has been shown to mediate nuclear export of profilin-actin complexes. A pseudogene of this gene is located on the long arm of chromosome 14. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Aug 2012]