p95 NBS1 (NBN) (NM_002485) Human Tagged ORF Clone
CAT#: RC214682
NBN (Myc-DDK-tagged)-Human nibrin (NBN)
ORF Plasmid: tGFP
Lentiviral Particles: DDK DDK w/ Puro mGFP mGFP w/ Puro
"NM_002485" in other vectors (6)
USD 447.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | p95 NBS1 |
Synonyms | AT-V1; AT-V2; ATV; NBS; NBS1; P95 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC214682 representing NM_002485
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGAAACTGCTGCCCGCCGCGGGCCCGGCAGGAGGAGAACCATACAGACTTTTGACTGGCGTTGAGT ACGTTGTTGGAAGGAAAAACTGTGCCATTCTGATTGAAAATGATCAGTCGATCAGCCGAAATCATGCTGT GTTAACTGCTAACTTTTCTGTAACCAACCTGAGTCAAACAGATGAAATCCCTGTATTGACATTAAAAGAT AATTCTAAGTATGGTACCTTTGTTAATGAGGAAAAAATGCAGAATGGCTTTTCCCGAACTTTGAAGTCGG GGGATGGTATTACTTTTGGAGTGTTTGGAAGTAAATTCAGAATAGAGTATGAGCCTTTGGTTGCATGCTC TTCTTGTTTAGATGTCTCTGGGAAAACTGCTTTAAATCAAGCTATATTGCAACTTGGAGGATTTACTGTA AACAATTGGACAGAAGAATGCACTCACCTTGTCATGGTATCAGTGAAAGTTACCATTAAAACAATATGTG CACTCATTTGTGGACGTCCAATTGTAAAGCCAGAATATTTTACTGAATTCCTGAAAGCAGTTGAGTCCAA GAAGCAGCCTCCACAAATTGAAAGTTTTTACCCACCTCTTGATGAACCATCTATTGGAAGTAAAAATGTT GATCTGTCAGGACGGCAGGAAAGAAAACAAATCTTCAAAGGGAAAACATTTATATTTTTGAATGCCAAAC AGCATAAGAAATTGAGTTCCGCAGTTGTCTTTGGAGGTGGGGAAGCTAGGTTGATAACAGAAGAGAATGA AGAAGAACATAATTTCTTTTTGGCTCCGGGAACGTGTGTTGTTGATACAGGAATAACAAACTCACAGACC TTAATTCCTGACTGTCAGAAGAAATGGATTCAGTCAATAATGGATATGCTCCAAAGGCAAGGTCTTAGAC CTATTCCTGAAGCAGAAATTGGATTGGCGGTGATTTTCATGACTACAAAGAATTACTGTGATCCTCAGGG CCATCCCAGTACAGGATTAAAGACAACAACTCCAGGACCAAGCCTTTCACAAGGCGTGTCAGTTGATGAA AAACTAATGCCAAGCGCCCCAGTGAACACTACAACATACGTAGCTGACACAGAATCAGAGCAAGCAGATA CATGGGATTTGAGTGAAAGGCCAAAAGAAATCAAAGTCTCCAAAATGGAACAAAAATTCAGAATGCTTTC ACAAGACGCACCCACTGTAAAGGAGTCCTGCAAAACAAGCTCTAATAATAATAGTATGGTATCAAATACT TTGGCTAAGATGAGAATCCCAAACTATCAGCTTTCACCAACTAAATTGCCAAGTATAAATAAAAGTAAAG ATAGGGCTTCTCAGCAGCAGCAGACCAACTCCATCAGAAACTACTTTCAGCCGTCTACCAAAAAAAGGGA AAGGGATGAAGAAAATCAAGAAATGTCTTCATGCAAATCAGCAAGAATAGAAACGTCTTGTTCTCTTTTA GAACAAACACAACCTGCTACACCCTCATTGTGGAAAAATAAGGAGCAGCATCTATCTGAGAATGAGCCTG TGGACACAAACTCAGACAATAACTTATTTACAGATACAGATTTAAAATCTATTGTGAAAAATTCTGCCAG TAAATCTCATGCTGCAGAAAAGCTAAGATCAAATAAAAAAAGGGAAATGGATGATGTGGCCATAGAAGAT GAAGTATTGGAACAGTTATTCAAGGACACAAAACCAGAGTTAGAAATTGATGTGAAAGTTCAAAAACAGG AGGAAGATGTCAATGTTAGAAAAAGGCCAAGGATGGATATAGAAACAAATGACACTTTCAGTGATGAAGC AGTACCAGAAAGTAGCAAAATATCTCAAGAAAATGAAATTGGGAAGAAACGTGAACTCAAGGAAGACTCA CTATGGTCAGCTAAAGAAATATCTAACAATGACAAACTTCAGGATGATAGTGAGATGCTTCCAAAAAAGC TGTTATTGACTGAATTTAGATCACTGGTGATTAAAAACTCTACTTCCAGAAATCCATCTGGCATAAATGA TGATTATGGTCAACTAAAAAATTTCAAGAAATTCAAAAAGGTCACATATCCTGGAGCAGGAAAACTTCCA CACATCATTGGAGGATCAGATCTAATAGCTCATCATGCTCGAAAGAATACAGAACTAGAAGAGTGGCTAA GGCAGGAAATGGAGGTACAAAATCAACATGCAAAAGAAGAGTCTCTTGCTGATGATCTTTTTAGATACAA TCCTTATTTAAAAAGGAGAAGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC214682 representing NM_002485
Red=Cloning site Green=Tags(s) MWKLLPAAGPAGGEPYRLLTGVEYVVGRKNCAILIENDQSISRNHAVLTANFSVTNLSQTDEIPVLTLKD NSKYGTFVNEEKMQNGFSRTLKSGDGITFGVFGSKFRIEYEPLVACSSCLDVSGKTALNQAILQLGGFTV NNWTEECTHLVMVSVKVTIKTICALICGRPIVKPEYFTEFLKAVESKKQPPQIESFYPPLDEPSIGSKNV DLSGRQERKQIFKGKTFIFLNAKQHKKLSSAVVFGGGEARLITEENEEEHNFFLAPGTCVVDTGITNSQT LIPDCQKKWIQSIMDMLQRQGLRPIPEAEIGLAVIFMTTKNYCDPQGHPSTGLKTTTPGPSLSQGVSVDE KLMPSAPVNTTTYVADTESEQADTWDLSERPKEIKVSKMEQKFRMLSQDAPTVKESCKTSSNNNSMVSNT LAKMRIPNYQLSPTKLPSINKSKDRASQQQQTNSIRNYFQPSTKKRERDEENQEMSSCKSARIETSCSLL EQTQPATPSLWKNKEQHLSENEPVDTNSDNNLFTDTDLKSIVKNSASKSHAAEKLRSNKKREMDDVAIED EVLEQLFKDTKPELEIDVKVQKQEEDVNVRKRPRMDIETNDTFSDEAVPESSKISQENEIGKKRELKEDS LWSAKEISNNDKLQDDSEMLPKKLLLTEFRSLVIKNSTSRNPSGINDDYGQLKNFKKFKKVTYPGAGKLP HIIGGSDLIAHHARKNTELEEWLRQEMEVQNQHAKEESLADDLFRYNPYLKRRR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_002485 |
ORF Size | 2262 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_002485.5 |
RefSeq Size | 4639 bp |
RefSeq ORF | 2265 bp |
Locus ID | 4683 |
UniProt ID | O60934 |
Cytogenetics | 8q21.3 |
Domains | FHA |
Protein Families | Druggable Genome |
Protein Pathways | Homologous recombination |
MW | 84.8 kDa |
Gene Summary | Mutations in this gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. This gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC214682L1 | Lenti ORF clone of Human nibrin (NBN), Myc-DDK-tagged |
USD 990.00 |
|
RC214682L2 | Lenti ORF clone of Human nibrin (NBN), mGFP tagged |
USD 990.00 |
|
RC214682L3 | Lenti ORF clone of Human nibrin (NBN), Myc-DDK-tagged |
USD 990.00 |
|
RC214682L4 | Lenti ORF clone of Human nibrin (NBN), mGFP tagged |
USD 990.00 |
|
RG214682 | NBN (tGFP-tagged) - Human nibrin (NBN) |
USD 890.00 |
|
SC118613 | NBN (untagged)-Human nibrin (NBN) |
USD 1,037.00 |
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