SALL4 (NM_020436) Human Tagged ORF Clone

CAT#: RC213089

SALL4 (Myc-DDK-tagged)-Human sal-like 4 (Drosophila) (SALL4)

Product Images

Western blot validation of overexpression lysate (Cat# LY412485) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC213089 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SALL4
• Synonyms: DRRS; HSAL4; IVIC; ZNF797
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213089 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGAGGCGCAAGCAGGCGAAACCCCAGCACATCAACTCGGAGGAGGACCAGGGCGAGCAGCAGCCGC
  AGCAGCAGACCCCGGAGTTTGCAGATGCGGCCCCAGCGGCGCCCGCGGCGGGGGAGCTGGGTGCTCCAGT
  GAACCACCCAGGGAATGACGAGGTGGCGAGTGAGGATGAAGCCACAGTAAAGCGGCTTCGTCGGGAGGAG
  ACGCACGTCTGTGAGAAATGCTGTGCGGAGTTCTTCAGCATCTCTGAGTTCCTGGAACATAAGAAAAATT
  GCACTAAAAATCCACCTGTCCTCATCATGAATGACAGCGAGGGGCCTGTGCCTTCAGAAGACTTCTCCGG
  AGCTGTACTGAGCCACCAGCCCACCAGTCCCGGCAGTAAGGACTGTCACAGGGAGAATGGCGGCAGCTCA
  GAGGACATGAAGGAGAAGCCGGATGCGGAGTCTGTGGTGTACCTAAAGACAGAGACAGCCCTGCCACCCA
  CCCCCCAGGACATAAGCTATTTAGCCAAAGGCAAAGTGGCCAACACTAACGTGACCTTGCAGGCACTACG
  GGGCACCAAGGTGGCGGTGAATCAGCGGAGCGCGGATGCACTCCCTGCCCCCGTGCCTGGTGCCAACAGC
  ATCCCGTGGGTCCTCGAGCAGATCTTGTGTCTGCAGCAGCAGCAGCTACAGCAGATCCAGCTCACCGAGC
  AGATCCGCATCCAGGTGAACATGTGGGCCTCCCACGCCCTCCACTCAAGCGGGGCAGGGGCCGACACTCT
  GAAGACCTTGGGCAGCCACATGTCTCAGCAGGTTTCTGCAGCTGTGGCTTTGCTCAGCCAGAAAGCTGGA
  AGCCAAGGTCTGTCTCTGGATGCCTTGAAACAAGCCAAGCTACCTCACGCCAACATCCCTTCTGCCACCA
  GCTCCCTGTCCCCAGGGCTGGCACCCTTCACTCTGAAGCCGGATGGGACCCGGGTGCTCCCGAACGTCAT
  GTCCCGCCTCCCGAGCGCTTTGCTTCCTCAGGCCCCGGGCTCGGTGCTCTTCCAGAGCCCTTTCTCCACT
  GTGGCACTAGACACATCCAAGAAAGGGAAGGGGAAGCCACCGAACATCTCCGCGGTGGATGTCAAACCCA
  AAGACGAGGCGGCCCTCTACAAGCACAAGTGTAAGTACTGTAGCAAGGTTTTTGGGACTGATAGCTCCTT
  GCAGATCCACCTCCGCTCCCACACTGGAGAGAGACCCTTCGTGTGCTCTGTCTGTGGTCATCGCTTCACC
  ACCAAGGGCAACCTCAAGGTGCACTTTCACCGACATCCCCAGGTGAAGGCAAACCCCCAGCTGTTTGCCG
  AGTTCCAGGACAAAGTGGCGGCCGGCAATGGCATCCCCTATGCACTCTCTGTACCTGACCCCATAGATGA
  ACCGAGTCTTTCTTTAGACAGCAAACCTGTCCTTGTAACCACCTCTGTAGGGCTACCTCAGAATCTTTCT
  TCGGGGACTAATCCCAAGGACCTCACGGGTGGCTCCTTGCCCGGTGACCTGCAGCCTGGGCCTTCTCCAG
  AAAGTGAGGGTGGACCCACACTCCCTGGGGTGGGACCAAACTATAATTCCCCAAGGGCTGGTGGCTTCCA
  AGGGAGTGGGACCCCTGAGCCAGGGTCAGAGACCCTGAAATTGCAGCAGTTGGTGGAGAACATTGACAAG
  GCCACCACTGATCCCAACGAATGTCTCATTTGCCACCGAGTCTTAAGCTGTCAGAGCTCCCTCAAGATGC
  ATTATCGCACCCACACCGGGGAGAGACCGTTCCAGTGTAAGATCTGTGGCCGAGCCTTTTCTACCAAAGG
  TAACCTGAAGACACACCTTGGGGTTCACCGAACCAACACATCCATTAAGACGCAGCATTCGTGCCCCATC
  TGCCAGAAGAAGTTCACTAATGCCGTGATGCTGCAGCAACATATTCGGATGCACATGGGCGGTCAGATTC
  CCAACACGCCCCTGCCAGAGAATCCCTGTGACTTTACGGGTTCTGAGCCAATGACCGTGGGTGAGAACGG
  CAGCACCGGCGCTATCTGCCATGATGATGTCATCGAAAGCATCGATGTAGAGGAAGTCAGCTCCCAGGAG
  GCTCCCAGCAGCTCCTCCAAGGTCCCCACGCCTCTTCCCAGCATCCACTCGGCATCACCCACGCTAGGGT
  TTGCCATGATGGCTTCCTTAGATGCCCCAGGGAAAGTGGGTCCTGCCCCTTTTAACCTGCAGCGCCAGGG
  CAGCAGAGAAAACGGTTCCGTGGAGAGCGATGGCTTGACCAACGACTCATCCTCGCTGATGGGAGACCAG
  GAGTATCAGAGCCGAAGCCCAGATATCCTGGAAACCACATCCTTCCAGGCACTCTCCCCGGCCAATAGTC
  AAGCCGAAAGCATCAAGTCAAAGTCTCCCGATGCTGGGAGCAAAGCAGAGAGCTCCGAGAACAGCCGCAC
  TGAGATGGAAGGTCGGAGCAGTCTCCCTTCCACGTTTATCCGAGCCCCGCCGACCTATGTCAAGGTTGAA
  GTTCCTGGCACATTTGTGGGACCCTCGACATTGTCCCCAGGGATGACCCCTTTGTTAGCAGCCCAGCCAC
  GCCGACAGGCCAAGCAACATGGCTGCACACGGTGTGGGAAGAACTTCTCCTCTGCTAGCGCTCTTCAGAT
  CCACGAGCGGACTCACACTGGAGAGAAGCCTTTTGTGTGCAACATTTGTGGGCGAGCTTTTACCACCAAA
  GGCAACTTAAAGGTTCACTACATGACACACGGGGCGAACAATAACTCAGCCCGCCGTGGAAGGAAGTTGG
  CCATCGAGAACACCATGGCTCTGTTAGGTACGGACGGAAAAAGAGTCTCAGAAATCTTTCCCAAGGAAAT
  CCTGGCCCCTTCAGTGAATGTGGACCCTGTTGTGTGGAACCAGTACACCAGCATGCTCAATGGCGGTCTG
  GCCGTGAAGACCAATGAGATCTCTGTGATCCAGAGTGGGGGGGTTCCTACCCTCCCGGTTTCCTTGGGGG
  CCACCTCCGTTGTGAATAACGCCACTGTCTCCAAGATGGATGGCTCCCAGTCGGGTATCAGTGCAGATGT
  GGAAAAACCAAGTGCTACTGACGGCGTTCCCAAACACCAGTTTCCTCACTTCCTGGAAGAAAACAAGATT
  GCGGTCAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213089 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSRRKQAKPQHINSEEDQGEQQPQQQTPEFADAAPAAPAAGELGAPVNHPGNDEVASEDEATVKRLRREE
  THVCEKCCAEFFSISEFLEHKKNCTKNPPVLIMNDSEGPVPSEDFSGAVLSHQPTSPGSKDCHRENGGSS
  EDMKEKPDAESVVYLKTETALPPTPQDISYLAKGKVANTNVTLQALRGTKVAVNQRSADALPAPVPGANS
  IPWVLEQILCLQQQQLQQIQLTEQIRIQVNMWASHALHSSGAGADTLKTLGSHMSQQVSAAVALLSQKAG
  SQGLSLDALKQAKLPHANIPSATSSLSPGLAPFTLKPDGTRVLPNVMSRLPSALLPQAPGSVLFQSPFST
  VALDTSKKGKGKPPNISAVDVKPKDEAALYKHKCKYCSKVFGTDSSLQIHLRSHTGERPFVCSVCGHRFT
  TKGNLKVHFHRHPQVKANPQLFAEFQDKVAAGNGIPYALSVPDPIDEPSLSLDSKPVLVTTSVGLPQNLS
  SGTNPKDLTGGSLPGDLQPGPSPESEGGPTLPGVGPNYNSPRAGGFQGSGTPEPGSETLKLQQLVENIDK
  ATTDPNECLICHRVLSCQSSLKMHYRTHTGERPFQCKICGRAFSTKGNLKTHLGVHRTNTSIKTQHSCPI
  CQKKFTNAVMLQQHIRMHMGGQIPNTPLPENPCDFTGSEPMTVGENGSTGAICHDDVIESIDVEEVSSQE
  APSSSSKVPTPLPSIHSASPTLGFAMMASLDAPGKVGPAPFNLQRQGSRENGSVESDGLTNDSSSLMGDQ
  EYQSRSPDILETTSFQALSPANSQAESIKSKSPDAGSKAESSENSRTEMEGRSSLPSTFIRAPPTYVKVE
  VPGTFVGPSTLSPGMTPLLAAQPRRQAKQHGCTRCGKNFSSASALQIHERTHTGEKPFVCNICGRAFTTK
  GNLKVHYMTHGANNNSARRGRKLAIENTMALLGTDGKRVSEIFPKEILAPSVNVDPVVWNQYTSMLNGGL
  AVKTNEISVIQSGGVPTLPVSLGATSVVNNATVSKMDGSQSGISADVEKPSATDGVPKHQFPHFLEENKI
  AVS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020436
• ORF Size: 3159 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020436.5
• RefSeq Size: 3487 bp
• RefSeq ORF: 3162 bp
• Locus ID: 57167
• UniProt ID: Q9UJQ4
• Cytogenetics: 20q13.2
• Protein Families: ES Cell Differentiation/IPS, Stem cell - Pluripotency
• MW: 112.2 kDa
• Gene Summary: This gene encodes a zinc finger transcription factor thought to play a role in the development of abducens motor neurons. Defects in this gene are a cause of Duane-radial ray syndrome (DRRS). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015]