IKBKE (NM_014002) Human Tagged ORF Clone

CAT#: RC212481

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IKBKE (Myc-DDK-tagged)-Human inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon (IKBKE), transcript variant 1



  "NM_014002" in other vectors (5)

Reconstitution Protocol

USD 656.00

In Stock*

Size
    • 10 ug

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Frequently bought together (4)
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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol IKBKE
Synonyms IKK-E; IKK-i; IKKE; IKKI
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC212481 representing NM_014002
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAGAGCACAGCCAATTACCTGTGGCACACAGATGACCTGCTGGGGCAGGGGGCCACTGCCAGTGTGT
ACAAGGCCCGCAACAAGAAATCCGGAGAGCTGGTTGCTGTGAAGGTCTTCAACACTACCAGCTACCTGCG
GCCCCGCGAGGTGCAGGTGAGGGAGTTTGAGGTCCTGCGGAAGCTGAACCACCAGAACATCGTCAAGCTC
TTTGCGGTGGAGGAGACGGGCGGAAGCCGGCAGAAGGTACTGGTGATGGAGTACTGCTCCAGTGGGAGCC
TGCTGAGTGTGCTGGAGAGCCCTGAGAATGCCTTTGGGCTGCCTGAGGATGAGTTCCTGGTGGTGCTGCG
CTGTGTGGTGGCCGGCATGAACCACCTGCGGGAGAACGGCATTGTGCATCGCGACATCAAGCCGGGGAAC
ATCATGCGCCTCGTAGGGGAGGAGGGGCAGAGCATCTACAAGCTGACAGACTTCGGCGCTGCCCGGGAGC
TGGATGATGATGAGAAGTTCGTCTCGGTCTATGGGACTGAGGAGTACCTGCATCCCGACATGTATGAGCG
GGCGGTGCTTCGAAAGCCCCAGCAAAAAGCGTTCGGGGTGACTGTGGATCTCTGGAGCATTGGAGTGACC
TTGTACCATGCAGCCACTGGCAGCCTGCCCTTCATCCCCTTTGGTGGGCCACGGCGGAACAAGGAGATCA
TGTACCGGATCACCACGGAGAAGCCGGCTGGGGCCATTGCAGGTGCCCAGAGGCGGGAGAACGGGCCCCT
GGAGTGGAGCTACACCCTCCCCATCACCTGCCAGCTGTCACTGGGGCTGCAGAGCCAGCTGGTGCCCATC
CTGGCCAACATCCTGGAGGTGGAGCAGGCCAAGTGCTGGGGCTTCGACCAGTTCTTTGCGGAGACCAGTG
ACATCCTGCAGCGAGTTGTCGTCCATGTCTTCTCCCTGTCCCAGGCAGTCCTGCACCACATCTATATCCA
TGCCCACAACACGATAGCCATTTTCCAGGAGGCCGTGCACAAGCAGACCAGTGTGGCCCCCCGACACCAG
GAGTACCTCTTTGAGGGTCACCTCTGTGTCCTCGAGCCCAGCGTCTCAGCACAGCACATCGCCCACACGA
CGGCAAGCAGCCCCCTGACCCTCTTCAGCACAGCCATCCCTAAGGGGCTGGCCTTCAGGGACCCTGCTCT
GGACGTCCCCAAGTTCGTCCCCAAAGTGGACCTGCAGGCGGATTACAACACTGCCAAGGGCGTGTTGGGC
GCCGGCTACCAGGCCCTGCGGCTGGCACGGGCCCTGCTGGATGGGCAGGAGCTAATGTTTCGGGGGCTGC
ACTGGGTCATGGAGGTGCTCCAGGCCACATGCAGACGGACTCTGGAAGTGGCAAGGACATCCCTCCTCTA
CCTCAGCAGCAGCCTGGGAACTGAGAGGTTCAGCAGCGTGGCTGGAACGCCTGAGATCCAGGAACTGAAG
GCGGCTGCAGAACTGAGGTCCAGGCTGCGGACTCTAGCGGAGGTCCTCTCCAGATGCTCCCAAAATATCA
CGGAGACCCAGGAGAGCCTGAGCAGCCTGAACCGGGAGCTGGTGAAGAGCCGGGATCAGGTACATGAGGA
CAGAAGCATCCAGCAGATTCAGTGCTGTTTGGACAAGATGAACTTCATCTACAAACAGTTCAAGAAGTCT
AGGATGAGGCCAGGGCTTGGCTACAACGAGGAGCAGATTCACAAGCTGGATAAGGTGAATTTCAGTCATT
TAGACAAAAGACTCCTGCAGGTGTTCCAGGAGGAGTGCGTGCAGAAGTATCAAGCGTCCTTAGTCACACA
CGGCAAGAGGATGAGGGTGGTGCACGAGACCAGGAACCACCTGCGCCTGGTTGGCTGTTCTGTGGCTGCC
TGTAACACAGAAGCCCAGGGGGTCCAGGAGAGTCTCAGCAAGCTCCTGGAAGAGCTATCTCACCAGCTCC
TTCAGGACCGAGCAAAGGGGGCTCAGGCCTCGCCGCCTCCCATAGCTCCTTACCCCAGCCCTACACGAAA
GGACCTGCTTCTCCACATGCAAGAGCTCTGCGAGGGGATGAAGCTGCTGGCATCTGACCTCCTGGACAAC
AACCGCATCATCGAACGGCTAAATAGAGTCCCAGCACCTCCTGATGTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC212481 representing NM_014002
Red=Cloning site Green=Tags(s)

MQSTANYLWHTDDLLGQGATASVYKARNKKSGELVAVKVFNTTSYLRPREVQVREFEVLRKLNHQNIVKL
FAVEETGGSRQKVLVMEYCSSGSLLSVLESPENAFGLPEDEFLVVLRCVVAGMNHLRENGIVHRDIKPGN
IMRLVGEEGQSIYKLTDFGAARELDDDEKFVSVYGTEEYLHPDMYERAVLRKPQQKAFGVTVDLWSIGVT
LYHAATGSLPFIPFGGPRRNKEIMYRITTEKPAGAIAGAQRRENGPLEWSYTLPITCQLSLGLQSQLVPI
LANILEVEQAKCWGFDQFFAETSDILQRVVVHVFSLSQAVLHHIYIHAHNTIAIFQEAVHKQTSVAPRHQ
EYLFEGHLCVLEPSVSAQHIAHTTASSPLTLFSTAIPKGLAFRDPALDVPKFVPKVDLQADYNTAKGVLG
AGYQALRLARALLDGQELMFRGLHWVMEVLQATCRRTLEVARTSLLYLSSSLGTERFSSVAGTPEIQELK
AAAELRSRLRTLAEVLSRCSQNITETQESLSSLNRELVKSRDQVHEDRSIQQIQCCLDKMNFIYKQFKKS
RMRPGLGYNEEQIHKLDKVNFSHLDKRLLQVFQEECVQKYQASLVTHGKRMRVVHETRNHLRLVGCSVAA
CNTEAQGVQESLSKLLEELSHQLLQDRAKGAQASPPPIAPYPSPTRKDLLLHMQELCEGMKLLASDLLDN
NRIIERLNRVPAPPDV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_014002
ORF Size 2148 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_014002.4
RefSeq Size 3263 bp
RefSeq ORF 2151 bp
Locus ID 9641
UniProt ID Q14164
Cytogenetics 1q32.1
Domains pkinase
Protein Families Druggable Genome, Protein Kinase
Protein Pathways Cytosolic DNA-sensing pathway, RIG-I-like receptor signaling pathway, Toll-like receptor signaling pathway
MW 80.3 kDa
Gene Summary IKBKE is a noncanonical I-kappa-B (see MIM 164008) kinase (IKK) that is essential for regulating antiviral signaling pathways. IKBKE has also been identified as a breast cancer (MIM 114480) oncogene and is amplified and overexpressed in over 30% of breast carcinomas and breast cancer cell lines (Hutti et al., 2009 [PubMed 19481526]).[supplied by OMIM, Oct 2009]

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