IKBKE (NM_014002) Human Untagged Clone

CAT#: SC323370

IKBKE (untagged)-Kinase deficient mutant (K38M) of Human inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon (IKBKE)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: IKBKE
• Synonyms: IKK-E; IKK-i; IKKE; IKKI
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323370 sequence for NM_014002 edited (data generated by NextGen Sequencing)
  ATGCAGAGCACAGCCAATTACCTGTGGCACACAGATGACCTGCTGGGGCAGGGGGCCACT
  GCCAGTGTGTACAAGGCCCGCAACAAGAAATCCGGAGAGCTGGTTGCTGTGATGGTCTTC
  AACACTACCAGCTACCTGCGGCCCCGCGAGGTGCAGGTGAGGGAGTTTGAGGTCCTGCGG
  AAGCTGAACCACCAGAACATCGTCAAGCTCTTTGCGGTGGAGGATACGGGCGGAAGCCGG
  CAGAAGGTACTGGTGATGGAGTACTGCTCCAGTGGGAGCCTGCTGAGTGTGCTGGAGAGC
  CCTGAGAATGCCTTTGGGCTGCCTGAGGATGAGTTCCTGGTGGTGCTGCGCTGTGTGGTG
  GCCGGCATGAACCACCTGCGGGAGAACGGCATTGTGCATCGCGACATCAAGCCGGGGAAC
  ATCATGCGCCTCGTAGGGGAGGAGGGGCAGAGCATCTACAAGCTGACAGACTTCGGCGCT
  GCCCGGGAGCTGGATGATGATGAGAAGTTCGTCTCGGTCTATGGGACTGAGGAGTACCTG
  CATCCCGACATGTATGAGCGGGCGGTGCTTCGAAAGCCCCAGCAAAAAGCGTTCGGGGTG
  ACTGTGGATCTCTGGAGCATTGGAGTGACCTTGTACCATGCAGCCACTGGCAGCCTGCCC
  TTCATCCCCTTTGGTGGGCCACGGCGGAACAAGGAGATCATGTACCGGATCACCACGGAG
  AAGCCGGCTGGGGCCATTGCAGGTGCCCAGAGGCGGGAGAACGGGCCCCTGGAGTGGAGC
  TACACCCTCCCCATCACCTGCCAGCTGTCACTGGGGCTGCAGAGCCAGCTGGTGCCCATC
  CTGGCCAACATCCTGGAGGTGGAGCAGGCCAAGTGCTGGGGCTTCGACCAGTTCTTTGCG
  GAGACCAGTGACATCCTGCAGCGAGTTGTCGTCCATGTCTTCTCCCTGTCCCAGGCAGTC
  CTGCACCACATCTATATCCATGCCCACAACACGATAGCCATTTTCCAGGAGGCCGTGCAC
  AAGCAGACCAGTGTGGCCCCCCGACACCAGGAGTACCTCTTTGAGGGTCACCTCTGTGTC
  CTCGAGCCCAGCGTCTCAGCACAGCACATCGCCCACACGACGGCAAGCAGCCCCCTGACC
  CTCTTCAGCACAGCCATCCCTAAGGGGCTGGCCTTCAGGGACCCTGCTCTGGACGTCCCC
  AAGTTCGTCCCCAAAGTGGACCTGCAGGCGGATTACAACACTGCCAAGGGCGTGTTGGGC
  GCCGGCTACCAGGCCCTGCGGCTGGCACGGGCCCTGCTGGATGGGCAGGAGCTAATGTTT
  CGGGGGCTGCACTGGGTCATGGAGGTGCTCCAGGCCACATGCAGACGGACTCTGGAAGTG
  GCAAGGACATCCCTCCTCTACCTCAGCAGCAGCCTGGGAACTGAGAGGTTCAGCAGCGTG
  GCTGGAACGCCTGAGATCCAGGAACTGAAGGCGGCTGCAGAACTGAGGTCCAGGCTGCGG
  ACTCTAGCGGAGGTCCTCTCCAGATGCTCCCAAAATATCACGGAGACCCAGGAGAGCCTG
  AGCAGCCTGAACCGGGAGCTGGTGAAGAGCCGGGATCAGGTACATGAGGACAGAAGCATC
  CAGCAGATTCAGTGCTGTTTGGACAAGATGAACTTCATCTACAAACAGTTCAAGAAGTCT
  AGGATGAGGCCAGGGCTTGGCTACAACGAGGAGCAGATTCACAAGCTGGATAAGGTGAAT
  TTCAGTCATTTAGCCAAAAGACTCCTGCAGGTGTTCCAGGAGGAGTGCGTGCAGAAGTAT
  CAAGCGTCCTTAGTCACACACGGCAAGAGGATGAGGGTGGTGCACGAGACCAGGAACCAC
  CTGCGCCTGGTTGGCTGTTCTGTGGCTGCCTGTAACACAGAAGCCCAGGGGGTCCAGGAG
  AGTCTCAGCAAGCTCCTGGAAGAGCTATCTCACCAGCTCCTTCAGGACCGAGCAAAGGGG
  GCTCAGGCCTCGCCGCCTCCCATAGCTCCTTACCCCAGCCCTACACGAAAGGACCTGCTT
  CTCCACATGCAAGAGCTCTGCGAGGGGATGAAGCTGCTGGCATCTGACCTCCTGGACAAC
  AACCGCATCATCGAACGGCTAAATAGAGTCCCAGCACTTCCTGATGTCTGA
  
  Clone variation with respect to NM_014002.3
  113 a=>t;201 t=>c;225 g=>t;2138 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_014002 unedited
  CCCGCCCGTTCGAGCAACTGGGCGGTAGGCGCTGTACGGTCTGTGGAGGTCTATATAAGCAGAGCTCGTT
  TAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGAAGAAACC
  CACTAGTCCCAGCTCCTGGGGTGGCACAGACATTGCAACTGGCCCTGCCTGTGGGTCCTAGGGGCCCTTG
  GCTACCAGGAGGCTAAGAACACTGCTCATGAATGACAGTGAGCCCTGAAAGCTCTGGGGGTGTCACCCAG
  TCCCACAAGCCTGCATCCCCTGCAGTGGAGATGGGCTCAGCTCCTGGACGTGCCACAGACAGAAAGCATA
  ACATACAGTTCGCCAGGAGAGCCTTTGCCTGACTCAGGGCAGCTTAAAGTTGGGGGCAGAAGTGACAGCC
  AGCTCAGGGCAGAAATGCAAAGCCAGCAATTACTGGTGGCAACGATAACTGGCGGGGCAGGGGCCCTGCA
  TTGTGTAAAGGCCGCAAAGAAATCGAAAACTGTTTGCTGTATGGTTTAAAATACAGCTACTGGGCCCCGA
  GGGTGAGTGAAGGATTTAAGGCCTGGGAACTAACCCCGAAACTCTCAGCTTTCGTGAGAGACCGGCGAAC
  CGCAAAGCTGTGTGAGACTCTCCCTGGACCCTGAGTGTGTGAACCGGAAGCCTGGTCGCAGAGATACCTG
  TGTCGCCTGTGGCGCGTACACTGGGAACGCTGCTCTGCTAACGGACATGCGTAGAGATGTACACTACAAA
  AAATGCGCGAAGGCGGT
  
  Kinase domain sequence

  >SC323370 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  CKACTGMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGT
  CAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGAAGAAACCCACTAGTCCCA
  GCTCCTGGGGTGGCACAGACATTGCAACTGGCCCTGCCTGTGGGTCCTAGGGGCCCTTGGCTACCAGGAG
  GCTAAGAACACTGCTCATGAATGACAGTGAGCCCTGAAAGCTCTG
  
• Restriction Sites: Please inquire
• ACCN: NM_014002
• Insert Size: 4000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014002.2, NP_054721.1
• RefSeq Size: 3263 bp
• RefSeq ORF: 2151 bp
• Locus ID: 9641
• UniProt ID: Q14164
• Cytogenetics: 1q32.1
• Domains: pkinase
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Cytosolic DNA-sensing pathway, RIG-I-like receptor signaling pathway, Toll-like receptor signaling pathway
• Gene Summary: IKBKE is a noncanonical I-kappa-B (see MIM 164008) kinase (IKK) that is essential for regulating antiviral signaling pathways. IKBKE has also been identified as a breast cancer (MIM 114480) oncogene and is amplified and overexpressed in over 30% of breast carcinomas and breast cancer cell lines (Hutti et al., 2009 [PubMed 19481526]).[supplied by OMIM, Oct 2009]
  Transcript Variant: This variant (1) is the longest transcript and it encodes the longest protein (isoform 1).