Sodium Iodide Symporter (SLC5A5) (NM_000453) Human Tagged ORF Clone

CAT#: RC211244

Reviews ()
Write a review

SLC5A5 (Myc-DDK-tagged)-Human solute carrier family 5 (sodium iodide symporter), member 5 (SLC5A5)



Reconstitution Protocol

USD 897.00

In Stock*

Size
    • 10 ug

Product images

Other products for "SLC5A5"

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol SLC5A5
Synonyms NIS; TDH1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC211244 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGCCGTGGAGACCGGGGAACGGCCCACCTTCGGAGCCTGGGACTACGGGGTCTTTGCCCTCATGC
TCCTGGTGTCCACTGGCATCGGGCTGTGGGTCGGGCTGGCTCGGGGCGGGCAGCGCAGCGCTGAGGACTT
CTTCACCGGGGGCCGGCGCCTGGCGGCCCTGCCCGTGGGCCTGTCGCTGTCTGCCAGCTTCATGTCGGCC
GTGCAGGTGCTGGGCGTGCCGTCGGAGGCCTATCGCTATGGCCTCAAGTTCCTCTGGATGTGCCTGGGCC
AGCTTCTGAACTCGGTCCTCACCGCCCTGCTCTTCATGCCCGTCTTCTACCGCCTGGGCCTCACCAGCAC
CTACGAGTACCTGGAGATGCGCTTCAGCCGCGCAGTGCGGCTCTGCGGGACTTTGCAGTACATTGTAGCC
ACGATGCTGTACACCGGCATCGTAATCTACGCACCGGCCCTCATCCTGAACCAAGTGACCGGGCTGGACA
TCTGGGCGTCGCTCCTGTCCACCGGAATTATCTGCACCTTCTACACGGCTGTGGGCGGCATGAAGGCTGT
GGTCTGGACTGATGTGTTCCAGGTCGTGGTGATGCTAAGTGGCTTCTGGGTTGTCCTGGCACGCGGTGTC
ATGCTTGTGGGCGGGCCCCGCCAGGTACTCACGCTGGCCCAGAACCACTCCCGGATCAACCTCATGGACT
TTAACCCTGACCCGAGGAGCCGCTATACATTCTGGACTTTTGTGGTGGGTGGCACGTTGGTGTGGCTCTC
CATGTATGGCGTGAACCAGGCGCAGGTGCAGCGCTACGTGGCTTGCCGCACAGAGAAGCAGGCCAAGCTG
GCCCTGCTCATCAACCAGGTCGGCCTGTTCCTGATCGTGTCCAGCGCTGCCTGCTGTGGCATCGTCATGT
TTGTGTTCTACACTGACTGCGACCCTCTCCTCCTGGGGCGCATCTCTGCCCCAGACCAGTACATGCCTCT
GCTGGTGCTGGACATCTTCGAAGATCTGCCTGGAGTCCCCGGGCTTTTCCTGGCCTGTGCTTACAGTGGC
ACCCTCAGCACAGCATCCACCAGCATCAATGCTATGGCTGCAGTCACTGTAGAAGACCTCATCAAACCTC
GGCTGCGGAGCCTGGCACCCAGGAAACTCGTGATTATCTCCAAGGGGCTCTCACTCATCTACGGATCGGC
CTGTCTCACCGTGGCAGCCCTGTCCTCACTGCTCGGAGGAGGTGTCCTTCAGGGCTCCTTCACCGTCATG
GGAGTCATCAGCGGCCCCCTGCTGGGAGCCTTCATCTTGGGAATGTTCCTGCCGGCCTGCAACACACCGG
GCGTCCTCGCGGGACTAGGCGCGGGCTTGGCGCTGTCGCTGTGGGTGGCCTTGGGCGCCACGCTGTACCC
ACCCAGCGAGCAGACCATGAGGGTCCTGCCATCGTCGGCTGCCCGCTGCGTGGCTCTCTCAGTCAACGCC
TCTGGCCTCCTGGACCCGGCTCTCCTCCCTGCTAACGACTCCAGCAGGGCCCCCAGCTCAGGAATGGACG
CCAGCCGACCCGCCTTAGCTGACAGCTTCTATGCCATCTCCTATCTCTATTACGGTGCCCTGGGCACGCT
GACCACTGTGCTGTGCGGAGCCCTCATCAGCTGCCTGACAGGCCCCACCAAGCGCAGCACCCTGGCCCCG
GGATTGTTGTGGTGGGACCTCGCACGGCAGACAGCATCAGTGGCCCCCAAGGAAGAAGTGGCCATCCTGG
ATGACAACTTGGTCAAGGGTCCTGAAGAACTCCCCACTGGAAACAAGAAGCCCCCTGGCTTCCTGCCCAC
CAATGAGGATCGTCTGTTTTTCTTGGGGCAGAAGGAGCTGGAGGGGGCTGGCTCTTGGACCCCCTGTGTT
GGACATGATGGTGGTCGAGACCAGCAGGAGACAAACCTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC211244 protein sequence
Red=Cloning site Green=Tags(s)

MEAVETGERPTFGAWDYGVFALMLLVSTGIGLWVGLARGGQRSAEDFFTGGRRLAALPVGLSLSASFMSA
VQVLGVPSEAYRYGLKFLWMCLGQLLNSVLTALLFMPVFYRLGLTSTYEYLEMRFSRAVRLCGTLQYIVA
TMLYTGIVIYAPALILNQVTGLDIWASLLSTGIICTFYTAVGGMKAVVWTDVFQVVVMLSGFWVVLARGV
MLVGGPRQVLTLAQNHSRINLMDFNPDPRSRYTFWTFVVGGTLVWLSMYGVNQAQVQRYVACRTEKQAKL
ALLINQVGLFLIVSSAACCGIVMFVFYTDCDPLLLGRISAPDQYMPLLVLDIFEDLPGVPGLFLACAYSG
TLSTASTSINAMAAVTVEDLIKPRLRSLAPRKLVIISKGLSLIYGSACLTVAALSSLLGGGVLQGSFTVM
GVISGPLLGAFILGMFLPACNTPGVLAGLGAGLALSLWVALGATLYPPSEQTMRVLPSSAARCVALSVNA
SGLLDPALLPANDSSRAPSSGMDASRPALADSFYAISYLYYGALGTLTTVLCGALISCLTGPTKRSTLAP
GLLWWDLARQTASVAPKEEVAILDDNLVKGPEELPTGNKKPPGFLPTNEDRLFFLGQKELEGAGSWTPCV
GHDGGRDQQETNL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_000453
ORF Size 1929 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_000453.3
RefSeq Size 3594 bp
RefSeq ORF 1932 bp
Locus ID 6528
UniProt ID Q92911
Cytogenetics 19p13.11
Protein Families Druggable Genome, Transmembrane
MW 68.7 kDa
Gene Summary This gene encodes a member of the sodium glucose cotransporter family. The encoded protein is responsible for the uptake of iodine in tissues such as the thyroid and lactating breast tissue. The iodine taken up by the thyroid is incorporated into the metabolic regulators triiodothyronine (T3) and tetraiodothyronine (T4). Mutations in this gene are associated with thyroid dyshormonogenesis 1.[provided by RefSeq, Sep 2009]
Customer Reviews 
Loading...
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.