Sodium Iodide Symporter (SLC5A5) (NM_000453) Human Tagged ORF Clone

CAT#: RG211244

SLC5A5 (tGFP-tagged) - Human solute carrier family 5 (sodium iodide symporter), member 5 (SLC5A5)

Product Images

Circular map for RG211244

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Sodium Iodide Symporter
• Synonyms: NIS; TDH1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG211244 representing NM_000453
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGCCGTGGAGACCGGGGAACGGCCCACCTTCGGAGCCTGGGACTACGGGGTCTTTGCCCTCATGC
  TCCTGGTGTCCACTGGCATCGGGCTGTGGGTCGGGCTGGCTCGGGGCGGGCAGCGCAGCGCTGAGGACTT
  CTTCACCGGGGGCCGGCGCCTGGCGGCCCTGCCCGTGGGCCTGTCGCTGTCTGCCAGCTTCATGTCGGCC
  GTGCAGGTGCTGGGCGTGCCGTCGGAGGCCTATCGCTATGGCCTCAAGTTCCTCTGGATGTGCCTGGGCC
  AGCTTCTGAACTCGGTCCTCACCGCCCTGCTCTTCATGCCCGTCTTCTACCGCCTGGGCCTCACCAGCAC
  CTACGAGTACCTGGAGATGCGCTTCAGCCGCGCAGTGCGGCTCTGCGGGACTTTGCAGTACATTGTAGCC
  ACGATGCTGTACACCGGCATCGTAATCTACGCACCGGCCCTCATCCTGAACCAAGTGACCGGGCTGGACA
  TCTGGGCGTCGCTCCTGTCCACCGGAATTATCTGCACCTTCTACACGGCTGTGGGCGGCATGAAGGCTGT
  GGTCTGGACTGATGTGTTCCAGGTCGTGGTGATGCTAAGTGGCTTCTGGGTTGTCCTGGCACGCGGTGTC
  ATGCTTGTGGGCGGGCCCCGCCAGGTACTCACGCTGGCCCAGAACCACTCCCGGATCAACCTCATGGACT
  TTAACCCTGACCCGAGGAGCCGCTATACATTCTGGACTTTTGTGGTGGGTGGCACGTTGGTGTGGCTCTC
  CATGTATGGCGTGAACCAGGCGCAGGTGCAGCGCTACGTGGCTTGCCGCACAGAGAAGCAGGCCAAGCTG
  GCCCTGCTCATCAACCAGGTCGGCCTGTTCCTGATCGTGTCCAGCGCTGCCTGCTGTGGCATCGTCATGT
  TTGTGTTCTACACTGACTGCGACCCTCTCCTCCTGGGGCGCATCTCTGCCCCAGACCAGTACATGCCTCT
  GCTGGTGCTGGACATCTTCGAAGATCTGCCTGGAGTCCCCGGGCTTTTCCTGGCCTGTGCTTACAGTGGC
  ACCCTCAGCACAGCATCCACCAGCATCAATGCTATGGCTGCAGTCACTGTAGAAGACCTCATCAAACCTC
  GGCTGCGGAGCCTGGCACCCAGGAAACTCGTGATTATCTCCAAGGGGCTCTCACTCATCTACGGATCGGC
  CTGTCTCACCGTGGCAGCCCTGTCCTCACTGCTCGGAGGAGGTGTCCTTCAGGGCTCCTTCACCGTCATG
  GGAGTCATCAGCGGCCCCCTGCTGGGAGCCTTCATCTTGGGAATGTTCCTGCCGGCCTGCAACACACCGG
  GCGTCCTCGCGGGACTAGGCGCGGGCTTGGCGCTGTCGCTGTGGGTGGCCTTGGGCGCCACGCTGTACCC
  ACCCAGCGAGCAGACCATGAGGGTCCTGCCATCGTCGGCTGCCCGCTGCGTGGCTCTCTCAGTCAACGCC
  TCTGGCCTCCTGGACCCGGCTCTCCTCCCTGCTAACGACTCCAGCAGGGCCCCCAGCTCAGGAATGGACG
  CCAGCCGACCCGCCTTAGCTGACAGCTTCTATGCCATCTCCTATCTCTATTACGGTGCCCTGGGCACGCT
  GACCACTGTGCTGTGCGGAGCCCTCATCAGCTGCCTGACAGGCCCCACCAAGCGCAGCACCCTGGCCCCG
  GGATTGTTGTGGTGGGACCTCGCACGGCAGACAGCATCAGTGGCCCCCAAGGAAGAAGTGGCCATCCTGG
  ATGACAACTTGGTCAAGGGTCCTGAAGAACTCCCCACTGGAAACAAGAAGCCCCCTGGCTTCCTGCCCAC
  CAATGAGGATCGTCTGTTTTTCTTGGGGCAGAAGGAGCTGGAGGGGGCTGGCTCTTGGACCCCCTGTGTT
  GGACATGATGGTGGTCGAGACCAGCAGGAGACAAACCTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG211244 representing NM_000453
  Red=Cloning site Green=Tags(s)
  
  MEAVETGERPTFGAWDYGVFALMLLVSTGIGLWVGLARGGQRSAEDFFTGGRRLAALPVGLSLSASFMSA
  VQVLGVPSEAYRYGLKFLWMCLGQLLNSVLTALLFMPVFYRLGLTSTYEYLEMRFSRAVRLCGTLQYIVA
  TMLYTGIVIYAPALILNQVTGLDIWASLLSTGIICTFYTAVGGMKAVVWTDVFQVVVMLSGFWVVLARGV
  MLVGGPRQVLTLAQNHSRINLMDFNPDPRSRYTFWTFVVGGTLVWLSMYGVNQAQVQRYVACRTEKQAKL
  ALLINQVGLFLIVSSAACCGIVMFVFYTDCDPLLLGRISAPDQYMPLLVLDIFEDLPGVPGLFLACAYSG
  TLSTASTSINAMAAVTVEDLIKPRLRSLAPRKLVIISKGLSLIYGSACLTVAALSSLLGGGVLQGSFTVM
  GVISGPLLGAFILGMFLPACNTPGVLAGLGAGLALSLWVALGATLYPPSEQTMRVLPSSAARCVALSVNA
  SGLLDPALLPANDSSRAPSSGMDASRPALADSFYAISYLYYGALGTLTTVLCGALISCLTGPTKRSTLAP
  GLLWWDLARQTASVAPKEEVAILDDNLVKGPEELPTGNKKPPGFLPTNEDRLFFLGQKELEGAGSWTPCV
  GHDGGRDQQETNL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000453
• ORF Size: 1929 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000453.1, NP_000444.1
• RefSeq Size: 2490 bp
• RefSeq ORF: 1932 bp
• Locus ID: 6528
• UniProt ID: Q92911
• Cytogenetics: 19p13.11
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: This gene encodes a member of the sodium glucose cotransporter family. The encoded protein is responsible for the uptake of iodine in tissues such as the thyroid and lactating breast tissue. The iodine taken up by the thyroid is incorporated into the metabolic regulators triiodothyronine (T3) and tetraiodothyronine (T4). Mutations in this gene are associated with thyroid dyshormonogenesis 1.[provided by RefSeq, Sep 2009]