PLA2G12B (NM_032562) Human Tagged ORF Clone
PLA2G12B (Myc-DDK-tagged)-Human phospholipase A2, group XIIB (PLA2G12B)
|Product Name||PLA2G12B (NM_032562) Human Tagged ORF Clone|
|Type||Human Tagged ORF Clone|
|Synonyms||FKSG71; GXIIB; GXIIIsPLA2; PLA2G13; sPLA2-GXIIB|
|E. coli Selection||Kanamycin (25 ug/mL)|
>RC210905 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)
>RC210905 protein sequence
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||585 bp|
|OTI Disclaimer||The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info|
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq||NM_032562.1, NM_032562.2, NM_032562.3, NM_032562.4, NP_115951.2|
|Protein Families||Secreted Protein|
|Protein Pathways||alpha-Linolenic acid metabolism, Arachidonic acid metabolism, Ether lipid metabolism, Fc epsilon RI signaling pathway, Glycerophospholipid metabolism, GnRH signaling pathway, Linoleic acid metabolism, Long-term depression, MAPK signaling pathway, Metabolic pathways, Vascular smooth muscle contraction, VEGF signaling pathway|
|Gene Summary||The protein encoded by this gene belongs to the phospholipase A2 (PLA2) group of enzymes, which function in glycolipid hydrolysis with the release of free fatty acids and lysophospholipids. This family member has altered phospholipid-binding properties and is catalytically inactive. The protein is secreted, and together with microsomal triglyceride transfer protein, it functions to regulate HNF4alpha-induced hepatitis C virus infectivity. The expression of this gene is down-regulated in various tumors, suggesting that it may function as a negative regulator of tumor progression. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Dec 2015]|
|cDNA Clone Resources|
|SC305474||PLA2G12B (untagged)-Human phospholipase A2, group XIIB (PLA2G12B)||
|RG210905||PLA2G12B (GFP-tagged) - Human phospholipase A2, group XIIB (PLA2G12B)||
|RC210905L1||Lenti ORF clone of Human phospholipase A2, group XIIB (PLA2G12B), Myc-DDK-tagged||
|RC210905L2||Lenti ORF clone of Human phospholipase A2, group XIIB (PLA2G12B), mGFP tagged||
|RC210905L3||Lenti ORF clone of Human phospholipase A2, group XIIB (PLA2G12B), Myc-DDK-tagged||
|RC210905L4||Lenti ORF clone of Human phospholipase A2, group XIIB (PLA2G12B), mGFP tagged||