IL2 (NM_000586) Human Tagged ORF Clone
IL2 (Myc-DDK-tagged)-Human interleukin 2 (IL2)
|Product Name||IL2 (NM_000586) Human Tagged ORF Clone|
|Type||Human Tagged ORF Clone|
|Synonyms||IL-2; lymphokine; TCGF|
|E. coli Selection||Kanamycin (25 ug/mL)|
>RC210013 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)
>RC210013 protein sequence
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||459 bp|
|OTI Disclaimer||The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info|
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq||NM_000586.2, NM_000586.3, NM_000586.4, NP_000577.2|
|RefSeq Size||822 bp|
|RefSeq ORF||462 bp|
|Protein Families||Druggable Genome, Secreted Protein|
|Protein Pathways||Allograft rejection, Autoimmune thyroid disease, Cytokine-cytokine receptor interaction, Graft-versus-host disease, Jak-STAT signaling pathway, T cell receptor signaling pathway, Type I diabetes mellitus|
|Gene Summary||The protein encoded by this gene is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. [provided by RefSeq, Jul 2008]|
|The use of this cDNA Clones has been cited in the following citations:|
Long Interleukin-22 Binding Protein Isoform-1 Is an Intracellular Activator of the Unfolded Protein Response
,Gómez-Fernández, P;Urtasun, A;Paton, AW;Paton, JC;Borrego, F;Dersh, D;Argon, Y;Alloza, I;Vandenbroeck, K;,
Front Immunol 2018
,PubMed ID 30619294
|cDNA Clone Resources|
|SC125234||IL2 (untagged)-Human interleukin 2 (IL2)||
|RG210013||IL2 (GFP-tagged) - Human interleukin 2 (IL2)||
|RC210013L1||Lenti ORF clone of Human interleukin 2 (IL2), Myc-DDK-tagged||
|RC210013L2||Lenti ORF clone of Human interleukin 2 (IL2), mGFP tagged||
|RC210013L3||Lenti ORF clone of Human interleukin 2 (IL2), Myc-DDK-tagged||
|RC210013L4||Lenti ORF clone of Human interleukin 2 (IL2), mGFP tagged||