GABA A Receptor gamma 2 (GABRG2) (NM_198904) Human Tagged ORF Clone

CAT#: RC209260

GABRG2 (Myc-DDK-tagged)-Human gamma-aminobutyric acid (GABA) A receptor, gamma 2 (GABRG2), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY403697) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209260 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GABRG2
• Synonyms: CAE2; DEE74; ECA2; EIEE74; FEB8; GEFSP3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209260 representing NM_198904
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTTCGCCAAATATATGGAGCACAGGAAGCTCAGTCTACTCGACTCCTGTATTTTCACAGAAAATGA
  CGGTGTGGATTCTGCTCCTGCTGTCGCTCTACCCTGGCTTCACTAGCCAGAAATCTGATGATGACTATGA
  AGATTATGCTTCTAACAAAACATGGGTCTTGACTCCAAAAGTTCCTGAGGGTGATGTCACTGTCATCTTA
  AACAACCTGCTGGAAGGATATGACAATAAACTTCGGCCTGATATAGGAGTGAAGCCAACGTTAATTCACA
  CAGACATGTATGTGAATAGCATTGGTCCAGTGAACGCTATCAATATGGAATACACTATTGATATATTTTT
  TGCGCAAACGTGGTATGACAGACGTTTGAAATTTAACAGCACCATTAAAGTCCTCCGATTGAACAGCAAC
  ATGGTGGGGAAAATCTGGATTCCAGACACTTTCTTCAGAAATTCCAAAAAAGCTGATGCACACTGGATCA
  CCACCCCCAACAGGATGCTGAGAATTTGGAATGATGGTCGAGTGCTCTACACCCTAAGGTTGACAATTGA
  TGCTGAGTGCCAATTACAATTGCACAACTTTCCAATGGATGAACACTCCTGCCCCTTGGAGTTCTCAAGT
  TATGGCTATCCACGTGAAGAAATTGTTTATCAATGGAAGCGAAGTTCTGTTGAAGTGGGCGACACAAGAT
  CCTGGAGGCTTTATCAATTCTCATTTGTTGGTCTAAGAAATACCACCGAAGTAGTGAAGACAACTTCCGG
  AGATTATGTGGTCATGTCTGTCTACTTTGATCTGAGCAGAAGAATGGGATACTTTACCATCCAGACCTAT
  ATCCCCTGCACACTCATTGTCGTCCTATCCTGGGTGTCTTTCTGGATCAATAAGGATGCTGTTCCAGCCA
  GAACATCTTTAGGTATCACCACTGTCCTGACAATGACCACCCTCAGCACCATTGCCCGGAAATCGCTCCC
  CAAGGTCTCCTATGTCACAGCGATGGATCTCTTTGTATCTGTTTGTTTCATCTTTGTCTTCTCTGCTCTG
  GTGGAGTATGGCACCTTGCGTTATTTTGTCAGCAACCGGAAACCAAGCAAGGACAAAGATAAAAAGAAGA
  AAAACCCTCTTCTTCGGATGTTTTCCTTCAAGGCCCCTACCATTGATATCCGCCCAAGATCAGCAACCAT
  TCAAATGAATAATGCTACACACCTTCAAGAGAGAGATGAAGAGTACGGCTATGAGTGTCTGGACGGCAAG
  GACTGTGCCAGTTTTTTCTGCTGTTTTGAAGATTGTCGAACAGGAGCTTGGAGACATGGGAGGATACATA
  TCCGCATTGCCAAAATGGACTCCTATGCTCGGATCTTCTTCCCCACTGCCTTCTGCCTGTTTAATCTGGT
  CTATTGGGTCTCCTACCTCTACCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209260 representing NM_198904
  Red=Cloning site Green=Tags(s)
  
  MSSPNIWSTGSSVYSTPVFSQKMTVWILLLLSLYPGFTSQKSDDDYEDYASNKTWVLTPKVPEGDVTVIL
  NNLLEGYDNKLRPDIGVKPTLIHTDMYVNSIGPVNAINMEYTIDIFFAQTWYDRRLKFNSTIKVLRLNSN
  MVGKIWIPDTFFRNSKKADAHWITTPNRMLRIWNDGRVLYTLRLTIDAECQLQLHNFPMDEHSCPLEFSS
  YGYPREEIVYQWKRSSVEVGDTRSWRLYQFSFVGLRNTTEVVKTTSGDYVVMSVYFDLSRRMGYFTIQTY
  IPCTLIVVLSWVSFWINKDAVPARTSLGITTVLTMTTLSTIARKSLPKVSYVTAMDLFVSVCFIFVFSAL
  VEYGTLRYFVSNRKPSKDKDKKKKNPLLRMFSFKAPTIDIRPRSATIQMNNATHLQERDEEYGYECLDGK
  DCASFFCCFEDCRTGAWRHGRIHIRIAKMDSYARIFFPTAFCLFNLVYWVSYLYL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_198904
• ORF Size: 1425 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198904.4
• RefSeq Size: 3871 bp
• RefSeq ORF: 1428 bp
• Locus ID: 2566
• UniProt ID: P18507
• Cytogenetics: 5q34
• Protein Families: Druggable Genome, Ion Channels: Cys-loop Receptors, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• MW: 50.8 kDa
• Gene Summary: This gene encodes a gamma-aminobutyric acid (GABA) receptor. GABA is the major inhibitory neurotransmitter in the mammlian brain, where it acts at GABA-A receptors, which are ligand-gated chloride channels. GABA-A receptors are pentameric, consisting of proteins from several subunit classes: alpha, beta, gamma, delta and rho. Mutations in this gene have been associated with epilepsy and febrile seizures. Multiple transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]