GPT2 (NM_133443) Human Tagged Lenti ORF Clone
CAT#: RC209119L4
- LentiORF®
Lenti ORF clone of Human glutamic pyruvate transaminase (alanine aminotransferase) 2 (GPT2), transcript variant 1, mGFP tagged
"NM_133443" in other vectors (5)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | mGFP |
Symbol | GPT2 |
Synonyms | ALT2; GPT 2; MRT49; NEDSPM |
Vector | pLenti-C-mGFP-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC209119).
|
Restriction Sites | SgfI-RsrII Cloning Scheme for this gene Plasmid Map |
ACCN | NM_133443 |
ORF Size | 1569 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_133443.1 |
RefSeq Size | 3963 bp |
RefSeq ORF | 1572 bp |
Locus ID | 84706 |
UniProt ID | Q8TD30 |
Cytogenetics | 16q11.2 |
Domains | aminotran_1_2 |
Protein Pathways | Alanine, aspartate and glutamate metabolism, Metabolic pathways |
MW | 57.9 kDa |
Gene Summary | This gene encodes a mitochondrial alanine transaminase, a pyridoxal enzyme that catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate. Alanine transaminases play roles in gluconeogenesis and amino acid metabolism in many tissues including skeletal muscle, kidney, and liver. Activating transcription factor 4 upregulates this gene under metabolic stress conditions in hepatocyte cell lines. A loss of function mutation in this gene has been associated with developmental encephalopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC209119 | GPT2 (Myc-DDK-tagged)-Human glutamic pyruvate transaminase (alanine aminotransferase) 2 (GPT2), transcript variant 1 |
USD 487.00 |
|
RC209119L3 | Lenti ORF clone of Human glutamic pyruvate transaminase (alanine aminotransferase) 2 (GPT2), transcript variant 1, Myc-DDK-tagged |
USD 787.00 |
|
RG209119 | GPT2 (tGFP-tagged) - Human glutamic pyruvate transaminase (alanine aminotransferase) 2 (GPT2), transcript variant 1 |
USD 687.00 |
|
SC120520 | GPT2 (untagged)-Human glutamic pyruvate transaminase (alanine aminotransferase) 2 (GPT2), transcript variant 1 |
USD 488.00 |
|
SC320514 | GPT2 (untagged)-Human glutamic pyruvate transaminase (alanine aminotransferase) 2 (GPT2), transcript variant 1 |
USD 488.00 |
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