GPT2 (NM_133443) Human Tagged ORF Clone

CAT#: RC209119

GPT2 (Myc-DDK-tagged)-Human glutamic pyruvate transaminase (alanine aminotransferase) 2 (GPT2), transcript variant 1

Product Images

Circular map for RC209119

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  Western blot validation of overexpression lysate (Cat# LY408784) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209119 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified GPT2 protein (Cat# TP309119). The protein was produced from HEK293T cells transfected with GPT2 cDNA clone (Cat# RC209119) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GPT2
• Synonyms: ALT2; GPT 2; MRT49; NEDSPM
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209119 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGCGGGCGGCGGCGCTGGTCCGGCGGGGCTGTGGTCCCCGGACCCCCAGCTCCTGGGGCCGCAGCC
  AGAGCAGCGCGGCCGCCGAGGCCTCGGCGGTGCTCAAGGTGCGGCCCGAGCGCAGCCGGCGCGAGCGCAT
  CCTCACGCTGGAGTCCATGAACCCGCAGGTGAAGGCGGTGGAGTACGCCGTGCGGGGACCCATCGTGCTC
  AAGGCCGGCGAGATCGAGCTCGAGCTGCAGCGGGGTATCAAAAAGCCATTCACAGAGGTCATCCGAGCCA
  ACATCGGGGACGCCCAGGCTATGGGGCAGCAGCCAATCACCTTCCTCCGGCAGGTGATGGCACTATGCAC
  CTACCCAAACCTGCTGGACAGCCCCAGCTTCCCAGAAGATGCTAAGAAACGTGCCCGGCGGATCCTGCAG
  GCTTGTGGCGGGAACAGCCTGGGGTCCTACAGTGCTAGCCAGGGTGTCAACTGCATCCGTGAAGATGTGG
  CTGCCTACATCACCAGGAGGGATGGCGGTGTGCCTGCGGACCCCGACAACATCTACCTGACCACGGGAGC
  TAGTGACGGCATTTCTACGATCCTGAAGATCCTCGTCTCCGGGGGCGGCAAGTCACGGACAGGTGTGATG
  ATCCCCATCCCACAATATCCCCTCTATTCAGCTGTCATCTCTGAGCTCGACGCCATCCAGGTGAATTACT
  ACCTGGACGAGGAGAACTGCTGGGCGCTGAATGTGAATGAGCTCCGGCGGGCGGTGCAGGAGGCCAAAGA
  CCACTGTGATCCTAAGGTGCTCTGCATAATCAACCCTGGGAACCCCACAGGCCAGGTACAAAGCAGAAAG
  TGCATAGAAGATGTGATCCACTTTGCCTGGGAAGAGAAGCTCTTTCTCCTGGCTGATGAGGTGTACCAGG
  ACAACGTGTACTCTCCAGATTGCAGATTCCACTCCTTCAAGAAGGTGCTGTACGAGATGGGGCCCGAGTA
  CTCCAGCAACGTGGAGCTCGCCTCCTTCCACTCCACCTCCAAGGGCTACATGGGCGAGTGTGGTTACAGA
  GGAGGCTACATGGAGGTGATCAACCTGCACCCTGAGATCAAGGGCCAGCTGGTGAAGCTGCTGTCGGTGC
  GCCTGTGCCCCCCAGTGTCTGGGCAGGCCGCCATGGACATTGTCGTGAACCCCCCGGTGGCAGGAGAGGA
  GTCCTTTGAGCAATTCAGCCGAGAGAAGGAGTCGGTCCTGGGTAATCTGGCCAAAAAAGCAAAGCTGACG
  GAAGACCTGTTTAACCAAGTCCCAGGAATTCACTGCAACCCCTTGCAGGGGGCCATGTACGCCTTCCCTC
  GGATCTTCATTCCTGCCAAAGCTGTGGAGGCTGCTCAGGCCCATCAAATGGCTCCAGACATGTTCTACTG
  CATGAAGCTCCTGGAGGAGACTGGCATCTGTGTCGTGCCCGGCAGTGGCTTTGGGCAGAGGGAAGGCACT
  TACCACTTCAGGATGACTATCCTCCCTCCAGTGGAGAAGCTGAAAACGGTGCTGCAGAAGGTGAAAGACT
  TCCACATCAACTTCCTGGAGAAGTACGCG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209119 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MQRAAALVRRGCGPRTPSSWGRSQSSAAAEASAVLKVRPERSRRERILTLESMNPQVKAVEYAVRGPIVL
  KAGEIELELQRGIKKPFTEVIRANIGDAQAMGQQPITFLRQVMALCTYPNLLDSPSFPEDAKKRARRILQ
  ACGGNSLGSYSASQGVNCIREDVAAYITRRDGGVPADPDNIYLTTGASDGISTILKILVSGGGKSRTGVM
  IPIPQYPLYSAVISELDAIQVNYYLDEENCWALNVNELRRAVQEAKDHCDPKVLCIINPGNPTGQVQSRK
  CIEDVIHFAWEEKLFLLADEVYQDNVYSPDCRFHSFKKVLYEMGPEYSSNVELASFHSTSKGYMGECGYR
  GGYMEVINLHPEIKGQLVKLLSVRLCPPVSGQAAMDIVVNPPVAGEESFEQFSREKESVLGNLAKKAKLT
  EDLFNQVPGIHCNPLQGAMYAFPRIFIPAKAVEAAQAHQMAPDMFYCMKLLEETGICVVPGSGFGQREGT
  YHFRMTILPPVEKLKTVLQKVKDFHINFLEKYA
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_133443
• ORF Size: 1569 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_133443.4
• RefSeq Size: 3963 bp
• RefSeq ORF: 1572 bp
• Locus ID: 84706
• UniProt ID: Q8TD30
• Cytogenetics: 16q11.2
• Domains: aminotran_1_2
• Protein Pathways: Alanine, aspartate and glutamate metabolism, Metabolic pathways
• MW: 57.9 kDa
• Gene Summary: This gene encodes a mitochondrial alanine transaminase, a pyridoxal enzyme that catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate. Alanine transaminases play roles in gluconeogenesis and amino acid metabolism in many tissues including skeletal muscle, kidney, and liver. Activating transcription factor 4 upregulates this gene under metabolic stress conditions in hepatocyte cell lines. A loss of function mutation in this gene has been associated with developmental encephalopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]