GPT2 (NM_133443) Human Tagged ORF Clone

CAT#: RC209119

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GPT2 (Myc-DDK-tagged)-Human glutamic pyruvate transaminase (alanine aminotransferase) 2 (GPT2), transcript variant 1



  "NM_133443" in other vectors (5)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 487.00


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Size
    • 10 ug


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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol GPT2
Synonyms ALT2; GPT 2; MRT49; NEDSPM
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC209119 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAGCGGGCGGCGGCGCTGGTCCGGCGGGGCTGTGGTCCCCGGACCCCCAGCTCCTGGGGCCGCAGCC
AGAGCAGCGCGGCCGCCGAGGCCTCGGCGGTGCTCAAGGTGCGGCCCGAGCGCAGCCGGCGCGAGCGCAT
CCTCACGCTGGAGTCCATGAACCCGCAGGTGAAGGCGGTGGAGTACGCCGTGCGGGGACCCATCGTGCTC
AAGGCCGGCGAGATCGAGCTCGAGCTGCAGCGGGGTATCAAAAAGCCATTCACAGAGGTCATCCGAGCCA
ACATCGGGGACGCCCAGGCTATGGGGCAGCAGCCAATCACCTTCCTCCGGCAGGTGATGGCACTATGCAC
CTACCCAAACCTGCTGGACAGCCCCAGCTTCCCAGAAGATGCTAAGAAACGTGCCCGGCGGATCCTGCAG
GCTTGTGGCGGGAACAGCCTGGGGTCCTACAGTGCTAGCCAGGGTGTCAACTGCATCCGTGAAGATGTGG
CTGCCTACATCACCAGGAGGGATGGCGGTGTGCCTGCGGACCCCGACAACATCTACCTGACCACGGGAGC
TAGTGACGGCATTTCTACGATCCTGAAGATCCTCGTCTCCGGGGGCGGCAAGTCACGGACAGGTGTGATG
ATCCCCATCCCACAATATCCCCTCTATTCAGCTGTCATCTCTGAGCTCGACGCCATCCAGGTGAATTACT
ACCTGGACGAGGAGAACTGCTGGGCGCTGAATGTGAATGAGCTCCGGCGGGCGGTGCAGGAGGCCAAAGA
CCACTGTGATCCTAAGGTGCTCTGCATAATCAACCCTGGGAACCCCACAGGCCAGGTACAAAGCAGAAAG
TGCATAGAAGATGTGATCCACTTTGCCTGGGAAGAGAAGCTCTTTCTCCTGGCTGATGAGGTGTACCAGG
ACAACGTGTACTCTCCAGATTGCAGATTCCACTCCTTCAAGAAGGTGCTGTACGAGATGGGGCCCGAGTA
CTCCAGCAACGTGGAGCTCGCCTCCTTCCACTCCACCTCCAAGGGCTACATGGGCGAGTGTGGTTACAGA
GGAGGCTACATGGAGGTGATCAACCTGCACCCTGAGATCAAGGGCCAGCTGGTGAAGCTGCTGTCGGTGC
GCCTGTGCCCCCCAGTGTCTGGGCAGGCCGCCATGGACATTGTCGTGAACCCCCCGGTGGCAGGAGAGGA
GTCCTTTGAGCAATTCAGCCGAGAGAAGGAGTCGGTCCTGGGTAATCTGGCCAAAAAAGCAAAGCTGACG
GAAGACCTGTTTAACCAAGTCCCAGGAATTCACTGCAACCCCTTGCAGGGGGCCATGTACGCCTTCCCTC
GGATCTTCATTCCTGCCAAAGCTGTGGAGGCTGCTCAGGCCCATCAAATGGCTCCAGACATGTTCTACTG
CATGAAGCTCCTGGAGGAGACTGGCATCTGTGTCGTGCCCGGCAGTGGCTTTGGGCAGAGGGAAGGCACT
TACCACTTCAGGATGACTATCCTCCCTCCAGTGGAGAAGCTGAAAACGGTGCTGCAGAAGGTGAAAGACT
TCCACATCAACTTCCTGGAGAAGTACGCG


AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAG
GTTTAA
>RC209119 protein sequence
Red=Cloning site Green=Tags(s)

MQRAAALVRRGCGPRTPSSWGRSQSSAAAEASAVLKVRPERSRRERILTLESMNPQVKAVEYAVRGPIVL
KAGEIELELQRGIKKPFTEVIRANIGDAQAMGQQPITFLRQVMALCTYPNLLDSPSFPEDAKKRARRILQ
ACGGNSLGSYSASQGVNCIREDVAAYITRRDGGVPADPDNIYLTTGASDGISTILKILVSGGGKSRTGVM
IPIPQYPLYSAVISELDAIQVNYYLDEENCWALNVNELRRAVQEAKDHCDPKVLCIINPGNPTGQVQSRK
CIEDVIHFAWEEKLFLLADEVYQDNVYSPDCRFHSFKKVLYEMGPEYSSNVELASFHSTSKGYMGECGYR
GGYMEVINLHPEIKGQLVKLLSVRLCPPVSGQAAMDIVVNPPVAGEESFEQFSREKESVLGNLAKKAKLT
EDLFNQVPGIHCNPLQGAMYAFPRIFIPAKAVEAAQAHQMAPDMFYCMKLLEETGICVVPGSGFGQREGT
YHFRMTILPPVEKLKTVLQKVKDFHINFLEKYA

SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_133443
ORF Size 1569 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_133443.4
RefSeq Size 3963 bp
RefSeq ORF 1572 bp
Locus ID 84706
UniProt ID Q8TD30
Cytogenetics 16q11.2
Domains aminotran_1_2
Protein Pathways Alanine, aspartate and glutamate metabolism, Metabolic pathways
MW 57.9 kDa
Gene Summary This gene encodes a mitochondrial alanine transaminase, a pyridoxal enzyme that catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate. Alanine transaminases play roles in gluconeogenesis and amino acid metabolism in many tissues including skeletal muscle, kidney, and liver. Activating transcription factor 4 upregulates this gene under metabolic stress conditions in hepatocyte cell lines. A loss of function mutation in this gene has been associated with developmental encephalopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]

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