BCL3 (NM_005178) Human Tagged ORF Clone

CAT#: RC209116

BCL3 (Myc-DDK-tagged)-Human B-cell CLL/lymphoma 3 (BCL3)

Product images

Western blot validation of overexpression lysate (Cat# LY417469) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209116 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified BCL3 protein (Cat# TP309116). The protein was produced from HEK293T cells transfected with BCL3 cDNA clone (Cat# RC209116) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BCL3
• Synonyms: BCL4; D19S37
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209116 representing NM_005178
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGAGGGGCCCGTGGACCTGCGCACCCGGCCCAAGGCCGCCGGACTCCCGGGCGCCGCGCTGCCGC
  TCCGCAAGCGCCCGCTGCGCGCGCCCTCCCCGGAGCCCGCCGCTCCCCGCGGCGCTGCGGGCCTTGTCGT
  CCCCCTGGACCCTCTGCGCGGCGGCTGCGACCTGCCGGCGGTCCCCGGGCCCCCCCACGGCCTGGCCCGG
  CCGGAGGCGCTTTACTACCCCGGAGCCTTACTGCCTTTGTACCCCACTCGGGCCATGGGCTCCCCGTTTC
  CTCTGGTGAACCTGCCTACACCCCTATACCCCATGATGTGCCCCATGGAACACCCCCTTTCTGCTGACAT
  CGCCATGGCCACCCGTGCAGATGAGGACGGAGACACGCCTCTCCATATTGCTGTGGTGCAGGGTAACCTG
  CCAGCTGTGCACCGGCTGGTCAACCTCTTCCAGCAGGGGGGCCGGGAGCTCGACATCTACAACAACCTAC
  GGCAGACACCGCTCCACCTGGCTGTGATCACCACATTACCGTCTGTGGTCCGGCTCCTGGTGACAGCTGG
  TGCCAGCCCCATGGCGCTGGACCGCCATGGCCAGACGGCCGCTCACCTGGCGTGCGAGCACCGCAGCCCG
  ACCTGCCTGCGAGCCCTGCTGGACAGCGCAGCTCCGGGCACGTTGGACCTGGAGGCCCGCAATTATGACG
  GGCTCACCGCCCTGCACGTGGCAGTGAACACCGAGTGCCAAGAAACCGTGCAGCTCTTGCTAGAGCGCGG
  TGCCGACATCGACGCAGTGGACATTAAGAGCGGCCGCTCCCCGCTCATCCACGCCGTGGAAAACAACAGC
  CTTAGCATGGTGCAGCTGCTGCTGCAGCACGGCGCCAACGTGAACGCGCAAATGTACTCCGGCAGCTCCG
  CCCTGCACTCAGCGTCCGGCCGCGGGCTCCTCCCGCTGGTGCGCACGCTGGTCCGCAGCGGCGCTGACAG
  CAGCCTCAAGAACTGCCACAACGACACGCCGCTCATGGTGGCGCGCAGCCGCAGGGTCATCGACATCCTG
  AGGGGGAAGGCCACCCGGCCTGCTTCCACCTCCCAGCCAGACCCCTCCCCTGACCGGAGCGCCAACACCT
  CCCCCGAGAGCAGCAGCCGCCTCAGCTCCAATGGTCTTCTCTCCGCATCACCATCCTCCTCACCCTCCCA
  GTCTCCCCCCAGGGACCCCCCTGGATTCCCCATGGCTCCTCCCAATTTCTTCCTTCCTTCCCCATCTCCA
  CCCGCCTTCCTGCCCTTTGCTGGGGTCCTCCGAGGCCCTGGCCGGCCGGTGCCCCCCTCCCCAGCTCCAG
  GAGGCAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209116 representing NM_005178
  Red=Cloning site Green=Tags(s)
  
  MDEGPVDLRTRPKAAGLPGAALPLRKRPLRAPSPEPAAPRGAAGLVVPLDPLRGGCDLPAVPGPPHGLAR
  PEALYYPGALLPLYPTRAMGSPFPLVNLPTPLYPMMCPMEHPLSADIAMATRADEDGDTPLHIAVVQGNL
  PAVHRLVNLFQQGGRELDIYNNLRQTPLHLAVITTLPSVVRLLVTAGASPMALDRHGQTAAHLACEHRSP
  TCLRALLDSAAPGTLDLEARNYDGLTALHVAVNTECQETVQLLLERGADIDAVDIKSGRSPLIHAVENNS
  LSMVQLLLQHGANVNAQMYSGSSALHSASGRGLLPLVRTLVRSGADSSLKNCHNDTPLMVARSRRVIDIL
  RGKATRPASTSQPDPSPDRSANTSPESSSRLSSNGLLSASPSSSPSQSPPRDPPGFPMAPPNFFLPSPSP
  PAFLPFAGVLRGPGRPVPPSPAPGGS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005178
• ORF Size: 1338 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005178.3, NP_005169.1
• RefSeq Size: 1864 bp
• RefSeq ORF: 1365 bp
• Locus ID: 602
• UniProt ID: P20749
• Cytogenetics: 19q13.32
• Domains: ANK
• Protein Families: Druggable Genome, Transcription Factors
• MW: 47.4 kDa
• Gene Summary: This gene is a proto-oncogene candidate. It is identified by its translocation into the immunoglobulin alpha-locus in some cases of B-cell leukemia. The protein encoded by this gene contains seven ankyrin repeats, which are most closely related to those found in I kappa B proteins. This protein functions as a transcriptional co-activator that activates through its association with NF-kappa B homodimers. The expression of this gene can be induced by NF-kappa B, which forms a part of the autoregulatory loop that controls the nuclear residence of p50 NF-kappa B. [provided by RefSeq, Jul 2008]