SFRP5 (NM_003015) Human Tagged ORF Clone

CAT#: RC208391

Reviews ()
Write a review

SFRP5 (Myc-DDK-tagged)-Human secreted frizzled-related protein 5 (SFRP5)



  "NM_003015" in other vectors (6)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 477.00


Availability*
In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol SFRP5
Synonyms SARP3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC208391 representing NM_003015
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGGGCGGCGGCGGCGGCGGGGGGCGTGCGGACGGCCGCGCTGGCGCTGCTGCTGGGGGCGCTGCACT
GGGCGCCGGCGCGCTGCGAGGAGTACGACTACTATGGCTGGCAGGCCGAGCCGCTGCACGGCCGCTCCTA
CTCCAAGCCGCCGCAGTGCCTTGACATCCCTGCCGACCTGCCGCTCTGCCACACGGTGGGCTACAAGCGC
ATGCGGCTGCCCAACCTGCTGGAGCACGAGAGCCTGGCCGAAGTGAAGCAGCAGGCGAGCAGCTGGCTGC
CGCTGCTGGCCAAGCGCTGCCACTCGGATACGCAGGTCTTCCTGTGCTCGCTCTTTGCGCCCGTCTGTCT
CGACCGGCCCATCTACCCGTGCCGCTCGCTGTGCGAGGCCGTGCGCGCCGGCTGCGCGCCGCTCATGGAG
GCCTACGGCTTCCCCTGGCCTGAGATGCTGCACTGCCACAAGTTCCCCCTGGACAACGACCTCTGCATCG
CCGTGCAGTTCGGGCACCTGCCCGCCACCGCGCCTCCAGTGACCAAGATCTGCGCCCAGTGTGAGATGGA
GCACAGTGCTGACGGCCTCATGGAGCAGATGTGCTCCAGTGACTTTGTGGTCAAAATGCGCATCAAGGAG
ATCAAGATAGAGAATGGGGACCGGAAGCTGATTGGAGCCCAGAAAAAGAAGAAGCTGCTCAAGCCGGGCC
CCCTGAAGCGCAAGGACACCAAGCGGCTGGTGCTGCACATGAAGAATGGCGCGGGCTGCCCCTGCCCACA
GCTGGACAGCCTGGCGGGCAGCTTCCTGGTCATGGGCCGCAAAGTGGATGGACAGCTGCTGCTCATGGCC
GTCTACCGCTGGGACAAGAAGAATAAGGAGATGAAGTTTGCAGTCAAATTCATGTTCTCCTACCCCTGCT
CCCTCTACTACCCTTTCTTCTACGGGGCGGCAGAGCCCCAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC208391 representing NM_003015
Red=Cloning site Green=Tags(s)

MRAAAAAGGVRTAALALLLGALHWAPARCEEYDYYGWQAEPLHGRSYSKPPQCLDIPADLPLCHTVGYKR
MRLPNLLEHESLAEVKQQASSWLPLLAKRCHSDTQVFLCSLFAPVCLDRPIYPCRSLCEAVRAGCAPLME
AYGFPWPEMLHCHKFPLDNDLCIAVQFGHLPATAPPVTKICAQCEMEHSADGLMEQMCSSDFVVKMRIKE
IKIENGDRKLIGAQKKKKLLKPGPLKRKDTKRLVLHMKNGAGCPCPQLDSLAGSFLVMGRKVDGQLLLMA
VYRWDKKNKEMKFAVKFMFSYPCSLYYPFFYGAAEPH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_003015
ORF Size 951 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_003015.2, NP_003006.1
RefSeq Size 1905 bp
RefSeq ORF 954 bp
Locus ID 6425
UniProt ID Q5T4F7
Cytogenetics 10q24.2
Protein Families Adult stem cells, Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein
Protein Pathways Wnt signaling pathway
MW 35.4 kDa
Gene Summary Secreted frizzled-related protein 5 (SFRP5) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. SFRP5 and SFRP1 may be involved in determining the polarity of photoreceptor cells in the retina. SFRP5 is highly expressed in the retinal pigment epithelium, and moderately expressed in the pancreas. [provided by RefSeq, Jul 2008]

Other Versions

Other products for "SFRP5"
Frequently bought together (5)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 30 ul

USD 165.00


pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

USD 627.00


Rabbit Polyclonal Anti-SFRP5 Antibody
    • 100 ul

USD 380.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

USD 149.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

USD 55.00

Customer Reviews 
Loading...
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.