IL7 (NM_000880) Human Tagged ORF Clone
IL7 (Myc-DDK-tagged)-Human interleukin 7 (IL7), transcript variant 1
|Product Name||IL7 (NM_000880) Human Tagged ORF Clone|
|Type||Human Tagged ORF Clone|
|E. coli Selection||Kanamycin (25 ug/mL)|
>RC208274 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)
>RC208274 protein sequence
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||531 bp|
|OTI Disclaimer||The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info|
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq||NM_000880.1, NM_000880.2, NM_000880.3, NP_000871.1|
|RefSeq Size||2089 bp|
|RefSeq ORF||534 bp|
|Protein Families||Druggable Genome, Secreted Protein|
|Protein Pathways||Cytokine-cytokine receptor interaction, Hematopoietic cell lineage, Jak-STAT signaling pathway|
|Gene Summary||The protein encoded by this gene is a cytokine important for B and T cell development. This cytokine and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. This cytokine is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRB) during early T cell development. This cytokine can be produced locally by intestinal epithelial and epithelial goblet cells, and may serve as a regulatory factor for intestinal mucosal lymphocytes. Knockout studies in mice suggested that this cytokine plays an essential role in lymphoid cell survival. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional splice variants have been described but their presence in normal tissues has not been confirmed. [provided by RefSeq, Dec 2010]|
|The use of this cDNA Clones has been cited in the following citations:|
An AAV Vector-Mediated Gene Delivery Approach Facilitates Reconstitution of Functional Human CD8(+) T Cells in Mice
,Huang, J;Li, X;Coelho-Dos-Reis, JG;Wilson, JM;Tsuji, M;,
PLoS ONE, Feb 2014.
,PubMed ID 24516613
|cDNA Clone Resources|
|SC126145||IL7 (untagged)-Human interleukin 7 (IL7), transcript variant 1||
|RG208274||IL7 (GFP-tagged) - Human interleukin 7 (IL7), transcript variant 1||
|RC208274L1||Lenti ORF clone of Human interleukin 7 (IL7), transcript variant 1, Myc-DDK-tagged||
|RC208274L2||Lenti ORF clone of Human interleukin 7 (IL7), transcript variant 1, mGFP tagged||
|RC208274L3||Lenti ORF clone of Human interleukin 7 (IL7), transcript variant 1, Myc-DDK-tagged||
|RC208274L4||Lenti ORF clone of Human interleukin 7 (IL7), transcript variant 1, mGFP tagged||