ACSL3 (NM_004457) Human Tagged ORF Clone

CAT#: RC208032

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ACSL3 (Myc-DDK-tagged)-Human acyl-CoA synthetase long-chain family member 3 (ACSL3), transcript variant 1



  "NM_004457" in other vectors (6)

USD 420.00


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Size
    • 10 ug


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Specifications

Product Data
Product Name ACSL3 (NM_004457) Human Tagged ORF Clone
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol ACSL3
Synonyms ACS3; FACL3; LACS 3; LACS3; PRO2194
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Cell Selection Neomycin
Sequence Data
>RC208032 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAATAACCACGTGTCTTCAAAACCATCTACCATGAAGCTAAAACATACCATCAACCCTATTCTTTTAT
ATTTTATACATTTTCTAATATCACTTTATACTATTTTAACATACATTCCGTTTTATTTTTTCTCCGAGTC
AAGACAAGAAAAATCAAACCGAATTAAAGCAAAGCCTGTAAATTCAAAACCTGATTCTGCATACAGATCT
GTTAATAGTTTGGATGGTTTGGCTTCAGTATTATACCCTGGATGTGATACTTTAGATAAAGTTTTTACAT
ATGCAAAAAACAAATTTAAGAACAAAAGACTCTTGGGAACACGTGAAGTTTTAAATGAGGAAGATGAAGT
ACAACCAAATGGAAAAATTTTTAAAAAGGTTATTCTTGGACAGTATAATTGGCTTTCCTATGAAGATGTC
TTTGTTCGAGCCTTTAATTTTGGAAATGGATTACAGATGTTGGGTCAGAAACCAAAGACCAACATCGCCA
TCTTCTGTGAGACCAGGGCCGAGTGGATGATAGCTGCACAGGCGTGTTTTATGTATAATTTTCAGCTTGT
TACATTATATGCCACTCTAGGAGGTCCAGCCATTGTTCATGCATTAAATGAAACAGAGGTGACCAACATC
ATTACTAGTAAAGAACTCTTACAAACAAAGTTGAAGGATATAGTTTCTTTGGTCCCACGCCTGCGGCACA
TCATCACTGTTGATGGAAAGCCACCGACCTGGTCCGAGTTCCCCAAGGGCATCATTGTGCATACCATGGC
TGCAGTGGAGGCCCTGGGAGCCAAGGCCAGCATGGAAAACCAACCTCATAGCAAACCATTGCCCTCAGAT
ATTGCAGTAATCATGTACACAAGTGGATCCACAGGACTTCCAAAGGGAGTCATGATCTCACATAGTAACA
TTATTGCTGGTATAACTGGGATGGCAGAAAGGATTCCAGAACTAGGAGAGGAAGATGTCTACATTGGATA
TTTGCCTCTGGCCCATGTTCTAGAATTAAGTGCTGAGCTTGTCTGTCTTTCTCACGGATGCCGCATTGGT
TACTCTTCACCACAGACTTTAGCAGATCAGTCTTCAAAAATTAAAAAAGGAAGCAAAGGGGATACATCCA
TGTTGAAACCAACACTGATGGCAGCAGTTCCGGAAATCATGGATCGGATCTACAAAAATGTCATGAATAA
AGTCAGTGAAATGAGTAGTTTTCAACGTAATCTGTTTATTCTGGCCTATAATTACAAAATGGAACAGATT
TCAAAAGGACGTAATACTCCACTGTGCGACAGCTTTGTTTTCCGGAAAGTTCGAAGCTTGCTAGGGGGAA
ATATTCGTCTCCTGTTGTGTGGTGGCGCTCCACTTTCTGCAACCACGCAGCGATTCATGAACATCTGTTT
CTGCTGTCCTGTTGGTCAGGGATACGGGCTCACTGAATCTGCTGGGGCTGGAACAATTTCCGAAGTGTGG
GACTACAATACTGGCAGAGTGGGAGCACCATTAGTTTGCTGTGAAATCAAATTAAAAAACTGGGAGGAAG
GTGGATACTTTAATACTGATAAGCCACACCCCAGGGGTGAAATTCTTATTGGGGGCCAAAGTGTGACAAT
GGGGTACTATAAAAATGAAGCAAAAACAAAAGCTGATTTCTTTGAAGATGAAAATGGACAAAGGTGGCTC
TGTACTGGGGATATTGGAGAGTTTGAACCCGATGGATGCTTAAAGATTATTGATCGTAAAAAGGACCTTG
TAAAACTACAGGCAGGGGAATATGTTTCTCTTGGGAAAGTAGAGGCAGCTTTGAAGAATCTTCCACTAGT
AGATAACATTTGTGCATATGCAAACAGTTATCATTCTTATGTCATTGGATTTGTTGTGCCAAATCAAAAG
GAACTAACTGAACTAGCTCGAAAGAAAGGACTTAAAGGGACTTGGGAGGAGCTGTGTAACAGTTGTGAAA
TGGAAAATGAGGTACTTAAAGTGCTTTCCGAAGCTGCTATTTCAGCAAGTCTGGAAAAGTTTGAAATTCC
AGTAAAAATTCGTTTGAGTCCTGAACCGTGGACCCCTGAAACTGGTCTGGTGACAGATGCCTTCAAGCTG
AAACGCAAAGAGCTTAAAACACATTACCAGGCGGACATTGAGCGAATGTATGGAAGAAAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC208032 protein sequence
Red=Cloning site Green=Tags(s)

MNNHVSSKPSTMKLKHTINPILLYFIHFLISLYTILTYIPFYFFSESRQEKSNRIKAKPVNSKPDSAYRS
VNSLDGLASVLYPGCDTLDKVFTYAKNKFKNKRLLGTREVLNEEDEVQPNGKIFKKVILGQYNWLSYEDV
FVRAFNFGNGLQMLGQKPKTNIAIFCETRAEWMIAAQACFMYNFQLVTLYATLGGPAIVHALNETEVTNI
ITSKELLQTKLKDIVSLVPRLRHIITVDGKPPTWSEFPKGIIVHTMAAVEALGAKASMENQPHSKPLPSD
IAVIMYTSGSTGLPKGVMISHSNIIAGITGMAERIPELGEEDVYIGYLPLAHVLELSAELVCLSHGCRIG
YSSPQTLADQSSKIKKGSKGDTSMLKPTLMAAVPEIMDRIYKNVMNKVSEMSSFQRNLFILAYNYKMEQI
SKGRNTPLCDSFVFRKVRSLLGGNIRLLLCGGAPLSATTQRFMNICFCCPVGQGYGLTESAGAGTISEVW
DYNTGRVGAPLVCCEIKLKNWEEGGYFNTDKPHPRGEILIGGQSVTMGYYKNEAKTKADFFEDENGQRWL
CTGDIGEFEPDGCLKIIDRKKDLVKLQAGEYVSLGKVEAALKNLPLVDNICAYANSYHSYVIGFVVPNQK
ELTELARKKGLKGTWEELCNSCEMENEVLKVLSEAAISASLEKFEIPVKIRLSPEPWTPETGLVTDAFKL
KRKELKTHYQADIERMYGRK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
Tag Myc-DDK
ACCN NM_004457
ORF Size 2160 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_004457.1, NM_004457.2, NM_004457.3, NP_004448.2
RefSeq Size 4369 bp
RefSeq ORF 2163 bp
Locus ID 2181
Cytogenetics 2q36.1
Domains AMP-binding
Protein Families Transmembrane
Protein Pathways Adipocytokine signaling pathway, Fatty acid metabolism, Metabolic pathways, PPAR signaling pathway
MW 80.4 kDa
Gene Summary The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme is highly expressed in brain, and preferentially utilizes myristate, arachidonate, and eicosapentaenoate as substrates. The amino acid sequence of this isozyme is 92% identical to that of rat homolog. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Citations (1)

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