ACSL3 (NM_004457) Human Untagged Clone

CAT#: SC117368

ACSL3 (untagged)-Human acyl-CoA synthetase long-chain family member 3 (ACSL3), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ACSL3
• Synonyms: ACS3; FACL3; LACS 3; LACS3; PRO2194
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_004457, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAATAACCACGTGTCTTCAAAACCATCTACCATGAAGCTAAAACATACCATCAACCCTATTCTTTTAT
  ATTTTATACATTTTCTAATATCACTTTATACTATTTTAACATACATTCCGTTTTATTTTTTCTCCGAGTC
  AAGACAAGAAAAATCAAACCGAATTAAAGCAAAGCCTGTAAATTCAAAACCTGATTCTGCATACAGATCT
  GTTAATAGTTTGGATGGTTTGGCTTCAGTATTATACCCTGGATGTGATACTTTAGATAAAGTTTTTACAT
  ATGCAAAAAACAAATTTAAGAACAAAAGACTCTTGGGAACACGTGAAGTTTTAAATGAGGAAGATGAAGT
  ACAACCAAATGGAAAAATTTTTAAAAAGGTTATTCTTGGACAGTATAATTGGCTTTCCTATGAAGATGTC
  TTTGTTCGAGCCTTTAATTTTGGAAATGGATTACAGATGTTGGGTCAGAAACCAAAGACCAACATCGCCA
  TCTTCTGTGAGACCAGGGCCGAGTGGATGATAGCTGCACAGGCGTGTTTTATGTATAATTTTCAGCTTGT
  TACATTATATGCCACTCTAGGAGGTCCAGCCATTGTTCATGCATTAAATGAAACAGAGGTGACCAACATC
  ATTACTAGTAAAGAACTCTTACAAACAAAGTTGAAGGATATAGTTTCTTTGGTCCCACGCCTGCGGCACA
  TCATCACTGTTGATGGAAAGCCACCGACCTGGTCCGAGTTCCCCAAGGGCATCATTGTGCATACCATGGC
  TGCAGTGGAGGCCCTGGGAGCCAAGGCCAGCATGGAAAACCAACCTCATAGCAAACCATTGCCCTCAGAT
  ATTGCAGTAATCATGTACACAAGTGGATCCACAGGACTTCCAAAGGGAGTCATGATCTCACATAGTAACA
  TTATTGCTGGTATAACTGGGATGGCAGAAAGGATTCCAGAACTAGGAGAGGAAGATGTCTACATTGGATA
  TTTGCCTCTGGCCCATGTTCTAGAATTAAGTGCTGAGCTTGTCTGTCTTTCTCACGGATGCCGCATTGGT
  TACTCTTCACCACAGACTTTAGCAGATCAGTCTTCAAAAATTAAAAAAGGAAGCAAAGGGGATACATCCA
  TGTTGAAACCAACACTGATGGCAGCAGTTCCGGAAATCATGGATCGGATCTACAAAAATGTCATGAATAA
  AGTCAGTGAAATGAGTAGTTTTCAACGTAATCTGTTTATTCTGGCCTATAATTACAAAATGGAACAGATT
  TCAAAAGGACGTAATACTCCACTGTGCGACAGCTTTGTTTTCCGGAAAGTTCGAAGCTTGCTAGGGGGAA
  ATATTCGTCTCCTGTTGTGTGGTGGCGCTCCACTTTCTGCAACCACGCAGCGATTCATGAACATCTGTTT
  CTGCTGTCCTGTTGGTCAGGGATACGGGCTCACTGAATCTGCTGGGGCTGGAACAATTTCCGAAGTGTGG
  GACTACAATACTGGCAGAGTGGGAGCACCATTAGTTTGCTGTGAAATCAAATTAAAAAACTGGGAGGAAG
  GTGGATACTTTAATACTGATAAGCCACACCCCAGGGGTGAAATTCTTATTGGGGGCCAAAGTGTGACAAT
  GGGGTACTACAAAAATGAAGCAAAAACAAAAGCTGATTTCTTTGAAGATGAAAATGGACAAAGGTGGCTC
  TGTACTGGGGATATTGGAGAGTTTGAACCCGATGGATGCTTAAAGATTATTGATCGTAAAAAGGACCTTG
  TAAAACTACAGGCAGGGGAATATGTTTCTCTTGGGAAAGTAGAGGCAGCTTTGAAGAATCTTCCACTAGT
  AGATAACATTTGTGCATATGCAAACAGTTATCATTCTTATGTCATTGGATTTGTTGTGCCAAATCAAAAG
  GAACTAACTGAACTAGCTCGAAAGAAAGGACTTAAAGGGACTTGGGAGGAGCTGTGTAACAGTTGTGAAA
  TGGAAAATGAGGTACTTAAAGTGCTTTCCGAAGCTGCTATTTCAGCAAGTCTGGAAAAGTTTGAAATTCC
  AGTAAAAATTCGTTTGAGTCCTGAACCGTGGACCCCTGAAACTGGTCTGGTGACAGATGCCTTCAAGCTG
  AAACGCAAAGAGCTTAAAACACATTACCAGGCGGACATTGAGCGAATGTATGGAAGAAAATAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004457 unedited
  GTTATAAATTGTATACGACTCATATAGGGCGGCCGCGATTCGGCACGAGGGGACGGGGTC
  GCATACGTTCGTCCCCTCGCATTGCGGCCCCGACAGCTGCGCCAGGATCCCCGGGCGGCG
  GCGCGGGGCGTGAACGCTCTGGGGCTCAGCCAGGCCTGCGCGGGCCCGAGGCCGGAGGAA
  CCCGGACTCCGGCGTAGCGGTTTTGACACAAGGGCGCATATCTTCAAAGCACCTAGTACC
  TCCTACCATTGTCAACTGATTCTCGCTGAAGTCTGTTAATTCTACTTTNTTGGAGTACTT
  ATGAATAACCACGTGTCTTCAAAACCATCTACCATGAAGCTAAAACATACCATCAACCCT
  ATTCTTTTATATTTTATACATTTTCTAATATCACTTTATACTATTTTAACATACATTCCG
  TTTTATTTTTTCTCCGAGTCAAGACAAGAAAAATCAAACCGAATTAAAGCAAAGCCTGTA
  AATTCAAAACCTGATTCTGCATACAGATCTGTTAATAGTTTGGATGGTTTGGCTTCAGTA
  TTATACCCTGGATGTGATACTTTAGATAAAGTTTTTACATATGCAAAAAACAAATTTAAG
  AACAAAAGACTCTTGGGAACACGTGAAGTTTTAAATGAGGAAGATGAAGTACAACCAAAT
  GGAAAAATTTTTAAAAGGTTATTCTTGGACAGTATAATGGNCTNTCCTATGAAAGATGTC
  TTGNTCGAGCCTTTAATTNNTGAAATGGATACAGATGTTGGGTCAGAAACAAAGACCACA
  TCGNCATCTTCTGTGAGACCAGGCCGAGTGGATGATAGCTGCAAGGCCTGTTTTATGTAT
  AAATTTCAGCTGGTACATAAATGGCACTCTAGGAGGTCCAGCCCATGTNATGCATTAAAT
  GAAN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004457 unedited
  GTGGATATGGGTATCTTTTATGNCTCTCAAAGCATACAAGCACATAATTACACAGCAACA
  AATTTTTTGTTCAACAATGATTTGATTCATAAGCATTTGAAATTTACATAATTTCATATC
  AATACCCTTGTATTTTTAAATACAGTAAGTAAAAAAGCCCCCAAATAACCAATTCTTATA
  TTTCCTATTTATCCCTCTATACATCCAAACTTTTAAAAAGTTACAAACTGAACATTATAC
  AGAACATATAAATCATGTTTAAAAACTTGAGGTTTTAAAATCACTGCTTCCCCAATATGA
  TTCACAAAAATTCTCATACTGACAAGTACAGCCATAGGTGGTAAAGTAAGTTGGTGGCGG
  GGAAAATGAAAGAAGACACAGACACAAGCCCGACTGCTTAATGGTTAACTAATCCTGCCA
  GTTAAAAATGGTGACGCCCTGAGAAGCCACAGCCCACTACTTTTACTGGCCCCTGCCATG
  CGCCTTGCTACATGCATTCCAAGTACTCCCCTATTCCATCTTGACCCTTTTCCCCACCCC
  GCCGTTCCGTTTACAATTAACCCTTTCCTTTTATATATTCCCTCAACGCTCGCCCGCTTT
  CCCGTTCCCTTCCTTCGCCTTCGTACTCCTTGATCCCCGTTACTTACTAATTTCCCCCGC
  CCCCCCCCCTCCTATTTCACCAATCGTTCTCGGACCCCAACACTTCCCATATTTCGCAAA
  TACAACCCCGGAACAAGTTGTCCTCTAATTCACCTATTACACCACTTTATACGCTCTTTT
  ACCCCCCCTTCGCCTTCCGACACGCCTTTTATTTTTTTCCTCCGCACCACCTATCACACC
  CTCTCCCGCCCGTGCCCGTTTCCCCCTCCCTAACCTTTCCCTCCCTCCACTATGTTTCCT
  CCCCTCCGCATCACCACTTTCTTCCCTTTCCCCCCGCCGCATATACCCCCGCGCTAAACG
  CTGCCCAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004457
• Insert Size: 3260 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004457.3, NP_004448.2
• RefSeq Size: 4369 bp
• RefSeq ORF: 2163 bp
• Locus ID: 2181
• UniProt ID: O95573
• Cytogenetics: 2q36.1
• Domains: AMP-binding
• Protein Families: Transmembrane
• Protein Pathways: Adipocytokine signaling pathway, Fatty acid metabolism, Metabolic pathways, PPAR signaling pathway
• Gene Summary: The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme is highly expressed in brain, and preferentially utilizes myristate, arachidonate, and eicosapentaenoate as substrates. The amino acid sequence of this isozyme is 92% identical to that of rat homolog. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein.