E2F4 (NM_001950) Human Tagged ORF Clone

CAT#: RC207699

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E2F4 (Myc-DDK-tagged)-Human E2F transcription factor 4, p107/p130-binding (E2F4)



  "NM_001950" in other vectors (4)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 457.00


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Size
    • 10 ug


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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol E2F4
Synonyms E2F-4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC207699 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGAGGCCGGGCCACAGGCGCCGCCGCCCCCGGGCACTCCAAGCCGGCACGAAAAGAGCCTGGGAC
TGCTCACCACCAAGTTCGTGTCCCTTCTGCAGGAGGCCAAGGACGGCGTGCTTGACCTCAAGCTGGCAGC
TGACACCCTAGCTGTACGCCAGAAGCGGCGGATTTACGACATTACCAATGTTTTGGAAGGTATCGGGCTA
ATCGAGAAAAAGTCCAAGAACAGCATCCAGTGGAAGGGTGTGGGGCCTGGCTGCAATACCCGGGAGATTG
CTGACAAACTGATTGAGCTCAAGGCAGAGATCGAGGAGCTGCAGCAGCGGGAGCAAGAACTAGACCAGCA
CAAGGTGTGGGTGCAGCAGAGCATCCGGAACGTCACAGAGGACGTGCAGAACAGCTGTTTGGCCTACGTC
ACTCATGAGGACATCTGCAGATGCTTTGCTGGAGATACCCTCTTGGCCATCCGGGCCCCATCAGGCACCA
GCCTGGAGGTGCCCATCCCAGAGGGTCTCAATGGGCAGAAGAAGTACCAGATTCACCTGAAGAGTGTGAG
TGGTCCCATTGAGGTTCTGCTGGTGAACAAGGAGGCATGGAGCTCACCCCCTGTGGCTGTGCCTGTGCCA
CCACCTGAAGATTTGCTCCAGAGCCCATCTGCTGTTTCTACACCTCCACCTCTGCCCAAGCCTGCCCTAG
CCCAGTCCCAGGAAGCCTCACGTCCAAATAGTCCTCAGCTCACTCCCACTGCTGTCCCTGGCAGTGCAGA
AGTCCAGGGAATGGCTGGCCCAGCAGCTGAGATCACAGTGAGTGGCGGCCCTGGGACTGATAGCAAGGAC
AGTGGTGAGCTCAGTTCACTCCCACTGGGCCCAACAACACTGGACACCCGGCCACTGCAGTCTTCTGCCC
TGCTGGACAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAACAGTAACAGCAGCAGTTCGTC
CGGACCCAACCCTTCTACCTCCTTTGAGCCCATCAAGGCAGACCCCACAGGTGTTTTGGAACTCCCCAAA
GAGCTGTCAGAAATCTTTGATCCCACACGAGAGTGCATGAGCTCGGAGCTGCTGGAGGAGTTGATGTCCT
CAGAAGTGTTTGCCCCTCTGCTTCGTCTTTCTCCACCCCCGGGAGACCACGATTATATCTACAACCTGGA
CGAGAGTGAAGGTGTCTGTGACCTCTTTGATGTGCCTGTTCTCAACCTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC207699 protein sequence
Red=Cloning site Green=Tags(s)

MAEAGPQAPPPPGTPSRHEKSLGLLTTKFVSLLQEAKDGVLDLKLAADTLAVRQKRRIYDITNVLEGIGL
IEKKSKNSIQWKGVGPGCNTREIADKLIELKAEIEELQQREQELDQHKVWVQQSIRNVTEDVQNSCLAYV
THEDICRCFAGDTLLAIRAPSGTSLEVPIPEGLNGQKKYQIHLKSVSGPIEVLLVNKEAWSSPPVAVPVP
PPEDLLQSPSAVSTPPPLPKPALAQSQEASRPNSPQLTPTAVPGSAEVQGMAGPAAEITVSGGPGTDSKD
SGELSSLPLGPTTLDTRPLQSSALLDSSSSSSSSSSSSSNSNSSSSSGPNPSTSFEPIKADPTGVLELPK
ELSEIFDPTRECMSSELLEELMSSEVFAPLLRLSPPPGDHDYIYNLDESEGVCDLFDVPVLNL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001950
ORF Size 1239 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001950.4
RefSeq Size 2100 bp
RefSeq ORF 1242 bp
Locus ID 1874
UniProt ID Q16254
Cytogenetics 16q22.1
Domains E2F_TDP
Protein Families Druggable Genome, Transcription Factors
Protein Pathways Cell cycle, TGF-beta signaling pathway
MW 44 kDa
Gene Summary The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein binds to all three of the tumor suppressor proteins pRB, p107 and p130, but with higher affinity to the last two. It plays an important role in the suppression of proliferation-associated genes, and its gene mutation and increased expression may be associated with human cancer. [provided by RefSeq, Jul 2008]

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