E2F4 (NM_001950) Human Tagged ORF Clone

CAT#: RC207699

E2F4 (Myc-DDK-tagged)-Human E2F transcription factor 4, p107/p130-binding (E2F4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY E2F4 (Cat# RC207699, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-E2F4 (Cat# TA811691)(1:500). Positive lysates LY400717 (100ug) and LC400717 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY400717) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207699 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E2F4
• Synonyms: E2F-4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207699 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAGGCCGGGCCACAGGCGCCGCCGCCCCCGGGCACTCCAAGCCGGCACGAAAAGAGCCTGGGAC
  TGCTCACCACCAAGTTCGTGTCCCTTCTGCAGGAGGCCAAGGACGGCGTGCTTGACCTCAAGCTGGCAGC
  TGACACCCTAGCTGTACGCCAGAAGCGGCGGATTTACGACATTACCAATGTTTTGGAAGGTATCGGGCTA
  ATCGAGAAAAAGTCCAAGAACAGCATCCAGTGGAAGGGTGTGGGGCCTGGCTGCAATACCCGGGAGATTG
  CTGACAAACTGATTGAGCTCAAGGCAGAGATCGAGGAGCTGCAGCAGCGGGAGCAAGAACTAGACCAGCA
  CAAGGTGTGGGTGCAGCAGAGCATCCGGAACGTCACAGAGGACGTGCAGAACAGCTGTTTGGCCTACGTC
  ACTCATGAGGACATCTGCAGATGCTTTGCTGGAGATACCCTCTTGGCCATCCGGGCCCCATCAGGCACCA
  GCCTGGAGGTGCCCATCCCAGAGGGTCTCAATGGGCAGAAGAAGTACCAGATTCACCTGAAGAGTGTGAG
  TGGTCCCATTGAGGTTCTGCTGGTGAACAAGGAGGCATGGAGCTCACCCCCTGTGGCTGTGCCTGTGCCA
  CCACCTGAAGATTTGCTCCAGAGCCCATCTGCTGTTTCTACACCTCCACCTCTGCCCAAGCCTGCCCTAG
  CCCAGTCCCAGGAAGCCTCACGTCCAAATAGTCCTCAGCTCACTCCCACTGCTGTCCCTGGCAGTGCAGA
  AGTCCAGGGAATGGCTGGCCCAGCAGCTGAGATCACAGTGAGTGGCGGCCCTGGGACTGATAGCAAGGAC
  AGTGGTGAGCTCAGTTCACTCCCACTGGGCCCAACAACACTGGACACCCGGCCACTGCAGTCTTCTGCCC
  TGCTGGACAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAACAGTAACAGCAGCAGTTCGTC
  CGGACCCAACCCTTCTACCTCCTTTGAGCCCATCAAGGCAGACCCCACAGGTGTTTTGGAACTCCCCAAA
  GAGCTGTCAGAAATCTTTGATCCCACACGAGAGTGCATGAGCTCGGAGCTGCTGGAGGAGTTGATGTCCT
  CAGAAGTGTTTGCCCCTCTGCTTCGTCTTTCTCCACCCCCGGGAGACCACGATTATATCTACAACCTGGA
  CGAGAGTGAAGGTGTCTGTGACCTCTTTGATGTGCCTGTTCTCAACCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207699 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAEAGPQAPPPPGTPSRHEKSLGLLTTKFVSLLQEAKDGVLDLKLAADTLAVRQKRRIYDITNVLEGIGL
  IEKKSKNSIQWKGVGPGCNTREIADKLIELKAEIEELQQREQELDQHKVWVQQSIRNVTEDVQNSCLAYV
  THEDICRCFAGDTLLAIRAPSGTSLEVPIPEGLNGQKKYQIHLKSVSGPIEVLLVNKEAWSSPPVAVPVP
  PPEDLLQSPSAVSTPPPLPKPALAQSQEASRPNSPQLTPTAVPGSAEVQGMAGPAAEITVSGGPGTDSKD
  SGELSSLPLGPTTLDTRPLQSSALLDSSSSSSSSSSSSSNSNSSSSSGPNPSTSFEPIKADPTGVLELPK
  ELSEIFDPTRECMSSELLEELMSSEVFAPLLRLSPPPGDHDYIYNLDESEGVCDLFDVPVLNL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001950
• ORF Size: 1239 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001950.4
• RefSeq Size: 2100 bp
• RefSeq ORF: 1242 bp
• Locus ID: 1874
• UniProt ID: Q16254
• Cytogenetics: 16q22.1
• Domains: E2F_TDP
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Cell cycle, TGF-beta signaling pathway
• MW: 44 kDa
• Gene Summary: The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein binds to all three of the tumor suppressor proteins pRB, p107 and p130, but with higher affinity to the last two. It plays an important role in the suppression of proliferation-associated genes, and its gene mutation and increased expression may be associated with human cancer. [provided by RefSeq, Jul 2008]