Lipin 1 (LPIN1) (NM_145693) Human Tagged ORF Clone

CAT#: RC207138

LPIN1 (Myc-DDK-tagged)-Human lipin 1 (LPIN1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY LPIN1 (Cat# RC207138, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LPIN1(Cat# TA805972). Positive lysates LY407897 (100ug) and LC407897 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY407897) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207138 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified LPIN1 protein (Cat# TP307138). The protein was produced from HEK293T cells transfected with LPIN1 cDNA clone (Cat# RC207138) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Lipin 1
• Synonyms: PAP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207138 representing NM_145693
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATTACGTGGGGCAGTTAGCCGGCCAGGTGTTTGTCACCGTGAAGGAGCTCTACAAGGGGCTGAATC
  CCGCCACACTCTCAGGGTGCATTGACATCATTGTCATCCGCCAGCCCAATGGAAACCTCCAATGCTCCCC
  TTTCCACGTCCGCTTTGGGAAGATGGGGGTCCTGCGCTCCCGAGAGAAAGTGGTTGACATAGAAATCAAT
  GGGGAATCTGTGGATTTGCATATGAAATTGGGAGATAATGGAGAAGCATTTTTTGTTCAAGAAACAGATA
  ATGATCAGGAAGTTATCCCTATGCACCTGGCCACCTCCCCCATCCTGTCAGAAGGAGCTTCGAGAATGGA
  ATGCCAGCTGAAAAGGGGCTCTGTGGACAGGATGAGAGGCCTGGACCCCAGCACGCCAGCCCAAGTGATC
  GCTCCCAGCGAGACGCCGTCAAGCAGCTCTGTAGTAAAGAAGAGAAGAAAAAGGAGGAGAAAGTCACAGC
  TGGACAGCCTGAAGAGAGATGACAACATGAACACATCTGAGGATGAGGACATGTTCCCCATCGAGATGAG
  CTCGGATGAGGCCATGGAGCTGCTGGAGAGCAGCAGAACTCTTCCTAATGATATACCTCCATTCCAAGAT
  GATATTCCTGAGGAAAACCTCTCCCTGGCTGTGATTTACCCTCAGTCAGCCTCATACCCTAATTCGGATA
  GAGAGTGGTCACCCACTCCCAGTCCTTCCGGTTCCCGACCTTCAACACCTAAAAGTGATTCAGAATTGGT
  CAGCAAGTCCACGGAAAGGACAGGGCAGAAGAACCCAGAAATGCTTTGGCTGTGGGGAGAGCTGCCGCAG
  GCTGCTAAGTCTTCTTCTCCACACAAGATGAAAGAGTCCAGCCCATTGAGCAGTAGAAAAATTTGTGATA
  AAAGTCACTTTCAGGCCATTCACAGCGAATCTTCAGACACTTTTAGTGACCAATCGCCAACTCTGGTCGG
  TGGGGCACTTTTGGACCAGAACAAGCCTCAGACAGAAATGCAGTTTGTGAATGAAGAAGACCTGGAGACC
  TTAGGAGCAGCAGCGCCACTCTTGCCCATGATCGAGGAGCTCAAACCCCCCTCTGCCAGTGTAGTCCAGA
  CAGCAAACAAGACGGATTCTCCTTCCAGGAAAAGAGATAAACGAAGCCGACATCTTGGTGCTGACGGCGT
  CTACTTGGATGACCTCACAGACATGGATCCTGAAGTGGCGGCCCTGTATTTTCCCAAAAACGGAGATCCT
  TCCGGACTCGCAAAACATGCAAGCGACAACGGAGCCCGGTCAGCCAACCAGTCCCCGCAGTCGGTGGGCA
  GCTCGGGCGTGGACAGTGGCGTGGAGAGCACCTCGGACGGGCTGAGGGACCTCCCTTCCATCGCCATCTC
  CCTCTGCGGGGGCCTCAGCGACCACCGGGAGATCACGAAAGATGCATTCCTGGAGCAAGCTGTGTCATAT
  CAACAGTTTGTGGACAACCCCGCTATTATCGATGACCCCAATCTCGTGGTAAAGATTGGGAGTAAATATT
  ATAACTGGACAACAGCAGCACCCCTCCTCCTGGCAATGCAGGCCTTCCAGAAACCTTTGCCAAAGGCCAC
  TGTGGAATCTATCATGAGGGATAAAATGCCCAAAAAGGGAGGAAGATGGTGGTTTTCATGGAGGGGAAGA
  AACACCACAATCAAGGAGGAAAGTAAGCCAGAGCAGTGCTTGGCTGGCAAGGCCCATAGCACCGGAGAGC
  AACCGCCGCAGCTCAGCTTGGCCACCAGGGTAAAGCATGAATCATCCTCCAGTGATGAGGAGCGCGCAGC
  TGCCAAGCCATCAAACGCAGGCCACCTCCCTCTTCTGCCTAATGTCAGCTACAAGAAGACTCTCCGGCTG
  ACTTCCGAGCAGCTTAAAAGCTTGAAGTTGAAGAATGGCCCCAACGACGTGGTTTTCAGTGTCACCACGC
  AGTACCAAGGCACGTGCCGCTGTGAGGGCACCATCTATCTGTGGAACTGGGATGATAAAGTCATCATTTC
  TGATATTGATGGGACAATTACCAGATCAGATACTCTTGGCCACATTTTGCCCACCCTTGGGAAGGATTGG
  ACCCATCAGGGCATCGCTAAGCTGTACCATAAAGTGAGCCAGAATGGATATAAATTTCTCTACTGTTCTG
  CCCGTGCCATCGGGATGGCGGACATGACGCGGGGCTACCTGCACTGGGTCAACGAGAGGGGCACGGTGCT
  GCCCCAGGGGCCCCTGCTGCTGAGTCCCAGCAGCCTCTTCTCTGCCCTGCACAGAGAAGTGATTGAAAAG
  AAGCCAGAAAAGTTTAAAGTCCAGTGTTTGACAGACATCAAAAACCTGTTTTTCCCCAACACAGAACCCT
  TTTATGCTGCTTTTGGAAACCGACCAGCTGATGTGTATTCATACAAGCAAGTAGGAGTGTCTTTGAATAG
  AATATTTACCGTCAACCCTAAAGGAGAGCTGGTACAGGAACATGCAAAGACCAACATCTCTTCGTATGTG
  AGACTCTGTGAAGTAGTCGACCACGTTTTCCCGTTGCTGAAAAGAAGCCATTCTTCAGACTTTCCCTGTT
  CGGATACCTTCAGTAACTTCACCTTTTGGAGAGAGCCACTGCCACCTTTTGAAAACCAGGACATTCATTC
  TGCCTCAGCG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207138 representing NM_145693
  Red=Cloning site Green=Tags(s)
  
  MNYVGQLAGQVFVTVKELYKGLNPATLSGCIDIIVIRQPNGNLQCSPFHVRFGKMGVLRSREKVVDIEIN
  GESVDLHMKLGDNGEAFFVQETDNDQEVIPMHLATSPILSEGASRMECQLKRGSVDRMRGLDPSTPAQVI
  APSETPSSSSVVKKRRKRRRKSQLDSLKRDDNMNTSEDEDMFPIEMSSDEAMELLESSRTLPNDIPPFQD
  DIPEENLSLAVIYPQSASYPNSDREWSPTPSPSGSRPSTPKSDSELVSKSTERTGQKNPEMLWLWGELPQ
  AAKSSSPHKMKESSPLSSRKICDKSHFQAIHSESSDTFSDQSPTLVGGALLDQNKPQTEMQFVNEEDLET
  LGAAAPLLPMIEELKPPSASVVQTANKTDSPSRKRDKRSRHLGADGVYLDDLTDMDPEVAALYFPKNGDP
  SGLAKHASDNGARSANQSPQSVGSSGVDSGVESTSDGLRDLPSIAISLCGGLSDHREITKDAFLEQAVSY
  QQFVDNPAIIDDPNLVVKIGSKYYNWTTAAPLLLAMQAFQKPLPKATVESIMRDKMPKKGGRWWFSWRGR
  NTTIKEESKPEQCLAGKAHSTGEQPPQLSLATRVKHESSSSDEERAAAKPSNAGHLPLLPNVSYKKTLRL
  TSEQLKSLKLKNGPNDVVFSVTTQYQGTCRCEGTIYLWNWDDKVIISDIDGTITRSDTLGHILPTLGKDW
  THQGIAKLYHKVSQNGYKFLYCSARAIGMADMTRGYLHWVNERGTVLPQGPLLLSPSSLFSALHREVIEK
  KPEKFKVQCLTDIKNLFFPNTEPFYAAFGNRPADVYSYKQVGVSLNRIFTVNPKGELVQEHAKTNISSYV
  RLCEVVDHVFPLLKRSHSSDFPCSDTFSNFTFWREPLPPFENQDIHSASA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145693
• ORF Size: 2670 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145693.4
• RefSeq Size: 5363 bp
• RefSeq ORF: 2673 bp
• Locus ID: 23175
• UniProt ID: Q14693
• Cytogenetics: 2p25.1
• Domains: lipin_N
• MW: 98.5 kDa
• Gene Summary: This gene encodes a magnesium-ion-dependent phosphatidic acid phosphohydrolase enzyme that catalyzes the penultimate step in triglyceride synthesis including the dephosphorylation of phosphatidic acid to yield diacylglycerol. Expression of this gene is required for adipocyte differentiation and it also functions as a nuclear transcriptional coactivator with some peroxisome proliferator-activated receptors to modulate expression of other genes involved in lipid metabolism. Mutations in this gene are associated with metabolic syndrome, type 2 diabetes, acute recurrent rhabdomyolysis, and autosomal recessive acute recurrent myoglobinuria (ARARM). This gene is also a candidate for several human lipodystrophy syndromes. [provided by RefSeq, Mar 2017]