Lipin 1 (LPIN1) (NM_145693) Human Untagged Clone

CAT#: SC125492

LPIN1 (untagged)-Human lipin 1 (LPIN1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Lipin 1
• Synonyms: PAP1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_145693 edited
  GGCAGCCGGCGGCGGGCTGGGTGTTTGCAATACAAAGGCGGCCACGCGCGGCGCCGCTCG
  GTGCAGACCATGAATTACGTGGGGCAGTTAGCCGGCCAGGTGTTTGTCACCGTGAAGGAG
  CTCTACAAGGGGCTGAATCCCGCCACACTCTCAGGGTGCATTGACATCATTGTCATCCGC
  CAGCCCAATGGAAACCTCCAATGCTCCCCTTTCCACGTCCGCTTTGGGAAGATGGGGGTC
  CTGCGCTCCCGAGAGAAAGTGGTTGACATAGAAATCAATGGGGAATCTGTGGATTTGCAT
  ATGAAATTGGGAGATAATGGAGAAGCATTTTTTGTTCAAGAAACAGATAATGATCAGGAA
  GTTATCCCTATGCACCTGGCCACCTCCCCCATCCTGTCAGAAGGAGCTTCGAGAATGGAA
  TGCCAGCTGAAAAGGGGCTCTGTGGACAGGATGAGAGGCCTGGACCCCAGCACGCCAGCC
  CAAGTGATCGCTCCCAGCGAGACGCCGTCAAGCAGCTCTGTAGTAAAGAAGAGAAGAAAA
  AGGAGGAGAAAGTCACAGCTGGACAGCCTGAAGAGAGATGACAACATGAACACATCTGAG
  GATGAGGACATGTTCCCCATCGAGATGAGCTCGGATGAGGCCATGGAGCTGCTGGAGAGC
  AGCAGAACTCTTCCTAATGATATACCTCCATTCCAAGATGATATTCCTGAGGAAAACCTC
  TCCCTGGCTGTGATTTACCCTCAGTCAGCCTCATACCCTAATTCGGATAGAGAGTGGTCA
  CCCACTCCCAGTCCTTCCGGTTCCCGACCTTCAACACCTAAAAGTGATTCAGAATTGGTC
  AGCAAGTCCACGGAAAGGACAGGGCAGAAGAACCCAGAAATGCTTTGGCTGTGGGGAGAG
  CTGCCGCAGGCTGCTAAGTCTTCTTCTCCACACAAGATGAAAGAGTCCAGCCCATTGAGC
  AGTAGAAAAATTTGTGATAAAAGTCACTTTCAGGCCATTCACAGCGAATCTTCAGACACT
  TTTAGTGACCAATCGCCAACTCTGGTCGGTGGGGCACTTTTGGACCAGAACAAGCCTCAG
  ACAGAAATGCAGTTTGTGAATGAAGAAGACCTGGAGACCTTAGGAGCAGCAGCGCCACTC
  TTGCCCATGATCGAGGAGCTCAAACCCCCCTCTGCCAGTGTAGTCCAGACAGCAAACAAG
  ACGGATTCTCCTTCCAGGAAAAGAGATAAACGAAGCCGACATCTTGGTGCTGACGGCGTC
  TACTTGGATGACCTCACAGACATGGATCCTGAAGTGGCGGCCCTGTATTTTCCCAAAAAC
  GGAGATCCTTCCGGACTCGCAAAACATGCAAGCGACAACGGAGCCCGGTCAGCCAACCAG
  TCCCCGCAGTCGGTGGGCAGCTCGGGCGTGGACAGTGGCGTGGAGAGCACCTCGGACGGG
  CTGAGGGACCTCCCTTCCATCGCCATCTCCCTCTGCGGGGGCCTCAGCGACCACCGGGAG
  ATCACGAAAGATGCATTCCTGGAGCAAGCTGTGTCATATCAACAGTTTGTGGACAACCCC
  GCTATTATCGATGACCCCAATCTCGTGGTAAAGATTGGGAGTAAATATTATAACTGGACA
  ACAGCAGCACCCCTCCTCCTGGCAATGCAGGCCTTCCAGAAACCTTTGCCAAAGGCCACT
  GTGGAATCTATCATGAGGGATAAAATGCCCAAAAAGGGAGGAAGATGGTGGTTTTCATGG
  AGGGGAAGAAACACCACAATCAAGGAGGAAAGTAAGCCAGAGCAGTGCTTGGCTGGCAAG
  GCCCATAGCACCGGAGAGCAACCGCCGCAGCTCAGCTTGGCCACCAGGGTAAAGCATGAA
  TCATCCTCCAGTGATGAGGAGCGCGCAGCTGCCAAGCCATCAAACGCAGGCCACCTCCCT
  CTTCTGCCTAATGTCAGCTACAAGAAGACTCTCCGGCTGACTTCCGAGCAGCTTAAAAGC
  TTGAAGTTGAAGAATGGCCCCAACGACGTGGTTTTCAGTGTCACCACGCAGTACCAAGGC
  ACGTGCCGCTGTGAGGGCACCATCTATCTGTGGAACTGGGATGATAAAGTCATCATTTCT
  GATATTGATGGGACAATTACCAGATCAGATACTCTTGGCCACATTTTGCCCACCCTTGGG
  AAGGATTGGACCCATCAGGGCATCGCTAAGCTGTACCATAAAGTGAGCCAGAATGGATAT
  AAATTTCTCTACTGTTCTGCCCGTGCCATCGGGATGGCGGACATGACGCGGGGCTACCTG
  CACTGGGTCAACGAGAGGGGCACGGTGCTGCCCCAGGGGCCCCTGCTGCTGAGTCCCAGC
  AGCCTCTTCTCTGCCCTGCACAGAGAAGTGATTGAAAAGAAGCCAGAAAAGTTTAAAGTC
  CAGTGTTTGACAGACATCAAAAACCTGTTTTTCCCCAACACAGAACCCTTTTATGCTGCT
  TTTGGAAACCGACCAGCTGATGTGTATTCATACAAGCAAGTAGGAGTGTCTTTGAATAGA
  ATATTTACCGTCAACCCTAAAGGAGAGCTGGTACAGGAACATGCAAAGACCAACATCTCT
  TCGTATGTGAGACTCTGTGAAGTAGTCGACCACGTTTTCCCGTTGCTGAAAAGAAGCCAT
  TCTTCAGACTTTCCCTGTTCGGATACCTTCAGTAACTTCACCTTTTGGAGAGAGCCACTG
  CCACCTTTTGAAAACCAGGACATTCATTCTGCCTCAGCGTAAAATGTCCCAAGCAGCCTC
  TTGCCAGCAGTGCAGAGCCTGGTTGTCACCCATTAAAGGATAGGTCTCCCCGGAGTGCAC
  AGCTCCACCTGGGAGCCTGGCGCGTCATCATTGGCCTGACAGCAGAGAGAATTGAGAAGC
  ATTTCTCCCCTGCCCCACCCCGGGGCTGACATTTCTAAGCAAGATAGGAAGGGAGCACTT
  TCTAGGCTAGGAGTTGGGTGCATTTGTACCGTGAAAAGCATTCCTCAGTTGTGGCTTAAT
  GCCAGTTACGACGCTGCCTTTCCG
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_145693 unedited
  GTTTTTAAATTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGCAGCCGGCG
  GCGGGCTGGGTGTTTGCAATACAAAGGCGGCCACGCGCGGCGCCGCTCGGTGCAGACCAT
  GAATTACGTGGGGCAGTTAGCCGGCCAGGTGTTTGTCACCGTGAAGGAGCTCTACAAGGG
  GCTGAATCCCGCCACACTCTCAGGGTGCATTGACATCATTGTCATCCGCCAGCCCAATGG
  AAACCTCCAATGCTCCCCTTTCCACGTCCGCTTTGGGAAGATGGGGGTCCTGCGCTCCCG
  AGAGAAAGTGGTTGACATAGAAATCAATGGGGAATCTGTGGATTTGCATATGAAATTGGG
  AGATAATGGAGAAGCATTTTTTGTTCAAGAAACAGATAATGATCAGGAAGTTATCCCTAT
  GCACCTGGCCACCTCCCCCATCCTGTCAGAAGGAGCTTCGAGAATGGAATGCCAGCTGAA
  AAGGGGCTCTGTGGACAGGATGAGAGGCCTGGACCCCAGCACGCCAGCCCAAGTGATCGC
  TCCCAGCGAGACGCCGTCAAGCAGCTCTGTAGTAAAGAAGAGAAGAAAAAGGAGGAGAAA
  GTCACAGCTGGACAGCCTGAAGAGAGATGACAACATGAACACATCTGANGATGAGGACAT
  GTTCCCCATCGAGATGAGCTCGGATGAGGCCATGGAGCTGCTGGAGAGCAGCAGAACTCT
  TCCTATGATATACCTNCATTCCAGATGATATTCCTGAGGAAAACCTCTCCCTGCTGTGAT
  TTACCCTCAGTCAGCCTCATACCCTAATTNCGATAGAGAGTGGTCACCCACTCCCAGTNC
  TTNCGNTTCCGACTTCNACACCTAAAGTGATTCAGAATTGTCAGCAGTCCACGAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_145693 unedited
  TGGACCGCGCGCCGTTTTTAGNANCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTGATT
  TGAGTCTTACTTTGTCACCCAGGCTGGAGTGCAGTAGTGCAAGCTCGGCTCACTGCAACC
  TCAGGCAGGACTATTTTTAATTATTTTTAATACCTGCAAAAGGGAATCTGCACATGCACA
  TCCGTGTTTCTACAGAAATCTGCGATCGATGGCAGATCTGTTTGCCTTTGGGTGTCCACA
  TGAACCATTTGGCAAAGGCATCCAATGCTAACGGGGCCCACCAACTACAACGGAGGCAAC
  AACTCTGTGGATTTTCTTTCACAGAAAGAGTAAAATTTCATTCAACCGTTCCATTTTTTG
  CCTCCTGTGATTCTAACAATGAAATTTAAATAGGAAAATGTTTCAATCGTTGGAGTAAAA
  CACTTTTTAAAAAAATAATGCTTCGAAAAGCAAAGCAAAATCTTTTACCAAAATAATTCT
  TTCACTTTTCAAATTGCCCTGTCCACGAGCCAGTGTAATAAACAAGTGAAAGAATCACAT
  TCTGTAGGCTTAAAGAATGCCAAATTATTACTTTTTCGCTTGCCCTTAATTTGAGTGGAA
  AAAGCTAAGGAACACACATGACTCATAAATTTCCAAAGAATGCCAGTCTATGAGAAAGAC
  AGAATAAGGCCCTTTCGATAGTCCAACACATACCTCTTTTTAATATATTAATGACAGCGG
  TATAAAACGAATATGAATAAATGCTTATCCCCGTACCCTAATCCGAGAAAACATTTCCTA
  CTTTTTAAATAATATAGTGACCTCTTGAAATATTTTCTAAACCAACACCTTCTTTGGAAA
  CCCCCACAGAGGCAGATCATTACATTTCACATAAGGGCGCCAAGTCCACTGTCGACACAC
  CTCCCTGAAGATAGAGATCGCCCGCCCACATTTTCAAAACCGCTTTACATATTCTATGCA
  CTTTACCTAAGCGTCGCA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_145693
• Insert Size: 4230 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145693.1, NP_663731.1
• RefSeq Size: 5363 bp
• RefSeq ORF: 2673 bp
• Locus ID: 23175
• UniProt ID: Q14693
• Cytogenetics: 2p25.1
• Domains: lipin_N
• Gene Summary: This gene encodes a magnesium-ion-dependent phosphatidic acid phosphohydrolase enzyme that catalyzes the penultimate step in triglyceride synthesis including the dephosphorylation of phosphatidic acid to yield diacylglycerol. Expression of this gene is required for adipocyte differentiation and it also functions as a nuclear transcriptional coactivator with some peroxisome proliferator-activated receptors to modulate expression of other genes involved in lipid metabolism. Mutations in this gene are associated with metabolic syndrome, type 2 diabetes, acute recurrent rhabdomyolysis, and autosomal recessive acute recurrent myoglobinuria (ARARM). This gene is also a candidate for several human lipodystrophy syndromes. [provided by RefSeq, Mar 2017]
  Transcript Variant: This variant (1) represents the shortest transcript and encodes the shortest isoform (1; also known as alpha). Both variants 1 and 5 encode the same isoform (1).