Lipin 1 (LPIN1) (NM_145693) Human Untagged Clone

SKU SC125492

LPIN1 (untagged)-Human lipin 1 (LPIN1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: Lipin 1
• Synonyms: PAP1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_145693 edited; GGCAGCCGGCGGCGGGCTGGGTGTTTGCAATACAAAGGCGGCCACGCGCGGCGCCGCTCG; GTGCAGACCATGAATTACGTGGGGCAGTTAGCCGGCCAGGTGTTTGTCACCGTGAAGGAG; CTCTACAAGGGGCTGAATCCCGCCACACTCTCAGGGTGCATTGACATCATTGTCATCCGC; CAGCCCAATGGAAACCTCCAATGCTCCCCTTTCCACGTCCGCTTTGGGAAGATGGGGGTC; CTGCGCTCCCGAGAGAAAGTGGTTGACATAGAAATCAATGGGGAATCTGTGGATTTGCAT; ATGAAATTGGGAGATAATGGAGAAGCATTTTTTGTTCAAGAAACAGATAATGATCAGGAA; GTTATCCCTATGCACCTGGCCACCTCCCCCATCCTGTCAGAAGGAGCTTCGAGAATGGAA; TGCCAGCTGAAAAGGGGCTCTGTGGACAGGATGAGAGGCCTGGACCCCAGCACGCCAGCC; CAAGTGATCGCTCCCAGCGAGACGCCGTCAAGCAGCTCTGTAGTAAAGAAGAGAAGAAAA; AGGAGGAGAAAGTCACAGCTGGACAGCCTGAAGAGAGATGACAACATGAACACATCTGAG; GATGAGGACATGTTCCCCATCGAGATGAGCTCGGATGAGGCCATGGAGCTGCTGGAGAGC; AGCAGAACTCTTCCTAATGATATACCTCCATTCCAAGATGATATTCCTGAGGAAAACCTC; TCCCTGGCTGTGATTTACCCTCAGTCAGCCTCATACCCTAATTCGGATAGAGAGTGGTCA; CCCACTCCCAGTCCTTCCGGTTCCCGACCTTCAACACCTAAAAGTGATTCAGAATTGGTC; AGCAAGTCCACGGAAAGGACAGGGCAGAAGAACCCAGAAATGCTTTGGCTGTGGGGAGAG; CTGCCGCAGGCTGCTAAGTCTTCTTCTCCACACAAGATGAAAGAGTCCAGCCCATTGAGC; AGTAGAAAAATTTGTGATAAAAGTCACTTTCAGGCCATTCACAGCGAATCTTCAGACACT; TTTAGTGACCAATCGCCAACTCTGGTCGGTGGGGCACTTTTGGACCAGAACAAGCCTCAG; ACAGAAATGCAGTTTGTGAATGAAGAAGACCTGGAGACCTTAGGAGCAGCAGCGCCACTC; TTGCCCATGATCGAGGAGCTCAAACCCCCCTCTGCCAGTGTAGTCCAGACAGCAAACAAG; ACGGATTCTCCTTCCAGGAAAAGAGATAAACGAAGCCGACATCTTGGTGCTGACGGCGTC; TACTTGGATGACCTCACAGACATGGATCCTGAAGTGGCGGCCCTGTATTTTCCCAAAAAC; GGAGATCCTTCCGGACTCGCAAAACATGCAAGCGACAACGGAGCCCGGTCAGCCAACCAG; TCCCCGCAGTCGGTGGGCAGCTCGGGCGTGGACAGTGGCGTGGAGAGCACCTCGGACGGG; CTGAGGGACCTCCCTTCCATCGCCATCTCCCTCTGCGGGGGCCTCAGCGACCACCGGGAG; ATCACGAAAGATGCATTCCTGGAGCAAGCTGTGTCATATCAACAGTTTGTGGACAACCCC; GCTATTATCGATGACCCCAATCTCGTGGTAAAGATTGGGAGTAAATATTATAACTGGACA; ACAGCAGCACCCCTCCTCCTGGCAATGCAGGCCTTCCAGAAACCTTTGCCAAAGGCCACT; GTGGAATCTATCATGAGGGATAAAATGCCCAAAAAGGGAGGAAGATGGTGGTTTTCATGG; AGGGGAAGAAACACCACAATCAAGGAGGAAAGTAAGCCAGAGCAGTGCTTGGCTGGCAAG; GCCCATAGCACCGGAGAGCAACCGCCGCAGCTCAGCTTGGCCACCAGGGTAAAGCATGAA; TCATCCTCCAGTGATGAGGAGCGCGCAGCTGCCAAGCCATCAAACGCAGGCCACCTCCCT; CTTCTGCCTAATGTCAGCTACAAGAAGACTCTCCGGCTGACTTCCGAGCAGCTTAAAAGC; TTGAAGTTGAAGAATGGCCCCAACGACGTGGTTTTCAGTGTCACCACGCAGTACCAAGGC; ACGTGCCGCTGTGAGGGCACCATCTATCTGTGGAACTGGGATGATAAAGTCATCATTTCT; GATATTGATGGGACAATTACCAGATCAGATACTCTTGGCCACATTTTGCCCACCCTTGGG; AAGGATTGGACCCATCAGGGCATCGCTAAGCTGTACCATAAAGTGAGCCAGAATGGATAT; AAATTTCTCTACTGTTCTGCCCGTGCCATCGGGATGGCGGACATGACGCGGGGCTACCTG; CACTGGGTCAACGAGAGGGGCACGGTGCTGCCCCAGGGGCCCCTGCTGCTGAGTCCCAGC; AGCCTCTTCTCTGCCCTGCACAGAGAAGTGATTGAAAAGAAGCCAGAAAAGTTTAAAGTC; CAGTGTTTGACAGACATCAAAAACCTGTTTTTCCCCAACACAGAACCCTTTTATGCTGCT; TTTGGAAACCGACCAGCTGATGTGTATTCATACAAGCAAGTAGGAGTGTCTTTGAATAGA; ATATTTACCGTCAACCCTAAAGGAGAGCTGGTACAGGAACATGCAAAGACCAACATCTCT; TCGTATGTGAGACTCTGTGAAGTAGTCGACCACGTTTTCCCGTTGCTGAAAAGAAGCCAT; TCTTCAGACTTTCCCTGTTCGGATACCTTCAGTAACTTCACCTTTTGGAGAGAGCCACTG; CCACCTTTTGAAAACCAGGACATTCATTCTGCCTCAGCGTAAAATGTCCCAAGCAGCCTC; TTGCCAGCAGTGCAGAGCCTGGTTGTCACCCATTAAAGGATAGGTCTCCCCGGAGTGCAC; AGCTCCACCTGGGAGCCTGGCGCGTCATCATTGGCCTGACAGCAGAGAGAATTGAGAAGC; ATTTCTCCCCTGCCCCACCCCGGGGCTGACATTTCTAAGCAAGATAGGAAGGGAGCACTT; TCTAGGCTAGGAGTTGGGTGCATTTGTACCGTGAAAAGCATTCCTCAGTTGTGGCTTAAT; GCCAGTTACGACGCTGCCTTTCCG;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_145693 unedited; GTTTTTAAATTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGCAGCCGGCG; GCGGGCTGGGTGTTTGCAATACAAAGGCGGCCACGCGCGGCGCCGCTCGGTGCAGACCAT; GAATTACGTGGGGCAGTTAGCCGGCCAGGTGTTTGTCACCGTGAAGGAGCTCTACAAGGG; GCTGAATCCCGCCACACTCTCAGGGTGCATTGACATCATTGTCATCCGCCAGCCCAATGG; AAACCTCCAATGCTCCCCTTTCCACGTCCGCTTTGGGAAGATGGGGGTCCTGCGCTCCCG; AGAGAAAGTGGTTGACATAGAAATCAATGGGGAATCTGTGGATTTGCATATGAAATTGGG; AGATAATGGAGAAGCATTTTTTGTTCAAGAAACAGATAATGATCAGGAAGTTATCCCTAT; GCACCTGGCCACCTCCCCCATCCTGTCAGAAGGAGCTTCGAGAATGGAATGCCAGCTGAA; AAGGGGCTCTGTGGACAGGATGAGAGGCCTGGACCCCAGCACGCCAGCCCAAGTGATCGC; TCCCAGCGAGACGCCGTCAAGCAGCTCTGTAGTAAAGAAGAGAAGAAAAAGGAGGAGAAA; GTCACAGCTGGACAGCCTGAAGAGAGATGACAACATGAACACATCTGANGATGAGGACAT; GTTCCCCATCGAGATGAGCTCGGATGAGGCCATGGAGCTGCTGGAGAGCAGCAGAACTCT; TCCTATGATATACCTNCATTCCAGATGATATTCCTGAGGAAAACCTCTCCCTGCTGTGAT; TTACCCTCAGTCAGCCTCATACCCTAATTNCGATAGAGAGTGGTCACCCACTCCCAGTNC; TTNCGNTTCCGACTTCNACACCTAAAGTGATTCAGAATTGTCAGCAGTCCACGAA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_145693 unedited; TGGACCGCGCGCCGTTTTTAGNANCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTGATT; TGAGTCTTACTTTGTCACCCAGGCTGGAGTGCAGTAGTGCAAGCTCGGCTCACTGCAACC; TCAGGCAGGACTATTTTTAATTATTTTTAATACCTGCAAAAGGGAATCTGCACATGCACA; TCCGTGTTTCTACAGAAATCTGCGATCGATGGCAGATCTGTTTGCCTTTGGGTGTCCACA; TGAACCATTTGGCAAAGGCATCCAATGCTAACGGGGCCCACCAACTACAACGGAGGCAAC; AACTCTGTGGATTTTCTTTCACAGAAAGAGTAAAATTTCATTCAACCGTTCCATTTTTTG; CCTCCTGTGATTCTAACAATGAAATTTAAATAGGAAAATGTTTCAATCGTTGGAGTAAAA; CACTTTTTAAAAAAATAATGCTTCGAAAAGCAAAGCAAAATCTTTTACCAAAATAATTCT; TTCACTTTTCAAATTGCCCTGTCCACGAGCCAGTGTAATAAACAAGTGAAAGAATCACAT; TCTGTAGGCTTAAAGAATGCCAAATTATTACTTTTTCGCTTGCCCTTAATTTGAGTGGAA; AAAGCTAAGGAACACACATGACTCATAAATTTCCAAAGAATGCCAGTCTATGAGAAAGAC; AGAATAAGGCCCTTTCGATAGTCCAACACATACCTCTTTTTAATATATTAATGACAGCGG; TATAAAACGAATATGAATAAATGCTTATCCCCGTACCCTAATCCGAGAAAACATTTCCTA; CTTTTTAAATAATATAGTGACCTCTTGAAATATTTTCTAAACCAACACCTTCTTTGGAAA; CCCCCACAGAGGCAGATCATTACATTTCACATAAGGGCGCCAAGTCCACTGTCGACACAC; CTCCCTGAAGATAGAGATCGCCCGCCCACATTTTCAAAACCGCTTTACATATTCTATGCA; CTTTACCTAAGCGTCGCA;
• Restriction Sites: NotI-NotI
• ACCN: NM_145693
• Insert Size: 4230 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_145693.1, NP_663731.1
• RefSeq Size: 5363 bp
• RefSeq ORF: 2673 bp
• Locus ID: 23175
• UniProt ID: Q14693
• Cytogenetics: 2p25.1
• Domains: lipin_N
• Summary: This gene encodes a magnesium-ion-dependent phosphatidic acid phosphohydrolase enzyme that catalyzes the penultimate step in triglyceride synthesis including the dephosphorylation of phosphatidic acid to yield diacylglycerol. Expression of this gene is required for adipocyte differentiation and it also functions as a nuclear transcriptional coactivator with some peroxisome proliferator-activated receptors to modulate expression of other genes involved in lipid metabolism. Mutations in this gene are associated with metabolic syndrome, type 2 diabetes, acute recurrent rhabdomyolysis, and autosomal recessive acute recurrent myoglobinuria (ARARM). This gene is also a candidate for several human lipodystrophy syndromes. [provided by RefSeq, Mar 2017]; Transcript Variant: This variant (1) represents the shortest transcript and encodes the shortest isoform (1; also known as alpha). Both variants 1 and 5 encode the same isoform (1).