CDRL2 (CDR2L) (NM_014603) Human Tagged ORF Clone

CAT#: RC206909

CDR2L (Myc-DDK-tagged)-Human cerebellar degeneration-related protein 2-like (CDR2L)

Product Images

Circular map for RC206909

• 

  Western blot validation of overexpression lysate (Cat# LY415158) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206909 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CDRL2
• Synonyms: HUMPPA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206909 representing NM_014603
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGAGAGCCGCCGGGATGGAGGACTTCTCCGCGGAGGAAGAGGAGTCCTGGTACGACCAGCAGGACC
  TGGAGCAGGACTTGCACCTAGCTGCGGAGCTGGGGAAGACTCTGCTGGAGAGGAACAAGGAGCTGGAGGG
  GTCCCTGCAGCAGATGTACTCCACCAATGAGGAACAGGTGCAGGAGATCGAGTACCTAACCAAGCAGCTG
  GACACGCTGCGGCACGTGAACGAGCAGCACGCCAAAGTCTATGAGCAGCTGGACCTGACAGCCCGGGACC
  TGGAGCTGACCAACCACAGGCTGGTGCTGGAGAGTAAGGCTGCCCAGCAGAAGATCCATGGGCTGACGGA
  GACCATTGAGCGCCTCCAGGCTCAGGTGGAGGAGCTGCAGGCCCAGGTGGAGCAACTGAGAGGCCTGGAA
  CAGCTGCGAGTGCTCCGGGAGAAGCGGGAACGCAGGCGTACCATCCACACCTTCCCCTGCCTCAAGGAGC
  TGTGCACCAGCCCCCGGTGCAAGGATGCTTTCCGCCTACACAGTTCCTCCCTGGAGCTGGGCCCGCGGCC
  CCTGGAGCAGGAGAACGAGCGGCTGCAGACCCTGGTGGGGGCGCTGCGCTCCCAGGTGAGCCAGGAGCGG
  CAGCGCAAGGAGCGGGCGGAGCGCGAGTACACCGCGGTGCTGCAGGAGTACTCGGAGCTGGAGCGCCAGC
  TGTGCGAGATGGAGGCCTGTCGCCTGCGTGTGCAGGAGCTGGAGGCCGAGCTGCTGGAGCTGCAGCAGAT
  GAAGCAGGCCAAGACCTACCTACTGGGTCCGGACGACCACCTGGCCGAGGCCCTGCTCGCACCCCTCACG
  CAGGCCCCTGAGGCCGACGATCCCCAGCCCGGCCGCGGGGACGACTTGGGCGCCCAGGACGGGGTCTCCT
  CACCGGCAGCCTCTCCAGGCCACGTGGTGCGCAAGAGCTGCAGCGACACTGCGCTCAACGCCATCGTGGC
  CAAAGACCCAGCCAGCCGGCACGCGGGCAACCTCACACTGCACGCCAACAGCGTGCGCAAGCGGGGCATG
  TCCATCCTGCGGGAGGTGGACGAGCAGTACCACGCGCTGCTGGAGAAGTACGAGGAGCTGCTGAGCAAGT
  GCCGGCAGCACGGGGCCGGAGTGCGGCACGCCGGCGTGCAGACCTCGCGCCCCATCTCCCGGGACAGCTC
  GTGGAGGGACCTGCGCGGGGGTGAGGAGGGCCAGGGTGAGGTCAAGGCAGGAGAGAAGAGCCTGAGCCAG
  CACGTGGAGGCCGTGGACAAGCGGCTGGAACAGAGCCAGCCCGAGTACAAGGCGCTCTTCAAAGAGATCT
  TCTCCAGGATCCAGAAGACCAAGGCTGACATCAACGCCACCAAAGTCAAGACGCACAGCAGCAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206909 representing NM_014603
  Red=Cloning site Green=Tags(s)
  
  MRRAAGMEDFSAEEEESWYDQQDLEQDLHLAAELGKTLLERNKELEGSLQQMYSTNEEQVQEIEYLTKQL
  DTLRHVNEQHAKVYEQLDLTARDLELTNHRLVLESKAAQQKIHGLTETIERLQAQVEELQAQVEQLRGLE
  QLRVLREKRERRRTIHTFPCLKELCTSPRCKDAFRLHSSSLELGPRPLEQENERLQTLVGALRSQVSQER
  QRKERAEREYTAVLQEYSELERQLCEMEACRLRVQELEAELLELQQMKQAKTYLLGPDDHLAEALLAPLT
  QAPEADDPQPGRGDDLGAQDGVSSPAASPGHVVRKSCSDTALNAIVAKDPASRHAGNLTLHANSVRKRGM
  SILREVDEQYHALLEKYEELLSKCRQHGAGVRHAGVQTSRPISRDSSWRDLRGGEEGQGEVKAGEKSLSQ
  HVEAVDKRLEQSQPEYKALFKEIFSRIQKTKADINATKVKTHSSK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014603
• ORF Size: 1395 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014603.3
• RefSeq Size: 3546 bp
• RefSeq ORF: 1398 bp
• Locus ID: 30850
• UniProt ID: Q86X02
• Cytogenetics: 17q25.1
• MW: 52.8 kDa