SKIV2L2 (MTREX) (NM_015360) Human Tagged Lenti ORF Clone

SKU
RC206080L4
Lenti-ORF clone of SKIV2L2 (mGFP-tagged)-Human superkiller viralicidic activity 2-like 2 (S. cerevisiae) (SKIV2L2)
  • LentiORF®
    LentiORF®

    Expression-ready ORF plasmid in lenti backbone

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$1,207.00
In Stock*
Specifications
Product Data
Target Symbol MTREX
Synonyms Dob1; fSAP118; KIAA0052; Mtr4; SKIV2L2
Vector pLenti-C-mGFP-P2A-Puro
E. coli Selection Chl (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(RC206080).
Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN NM_015360
ORF Size 3126 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_015360.4
RefSeq Size 4222 bp
RefSeq ORF 3129 bp
Locus ID 23517
UniProt ID P42285
Cytogenetics 5q11.2
Domains DEAD, helicase_C
Protein Pathways RNA degradation
MW 117.8 kDa
Summary Component of exosome targeting complexes. Subunit of the trimeric nuclear exosome targeting (NEXT) complex, a complex that directs a subset of non-coding short-lived RNAs for exosomal degradation. Subunit of the trimeric poly(A) tail exosome targeting (PAXT) complex, a complex that directs a subset of long and polyadenylated poly(A) RNAs for exosomal degradation. The RNA exosome is fundamental for the degradation of RNA in eukaryotic nuclei. Substrate targeting is facilitated by its cofactor MTREX, which links to RNA-binding protein adapters (PubMed:27871484). Associated with the RNA exosome complex and involved in the 3'-processing of the 7S pre-RNA to the mature 5.8S rRNA (PubMed:17412707, PubMed:29107693). May be involved in pre-mRNA splicing.[UniProtKB/Swiss-Prot Function]
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SKU Description Size Price
RC206080 SKIV2L2 (Myc-DDK-tagged)-Human superkiller viralicidic activity 2-like 2 (S. cerevisiae) (SKIV2L2) 10 ug
$907.00
RC206080L3 Lenti-ORF clone of SKIV2L2 (Myc-DDK-tagged)-Human superkiller viralicidic activity 2-like 2 (S. cerevisiae) (SKIV2L2) 10 ug
$1,207.00
RG206080 SKIV2L2 (tGFP-tagged) - Human superkiller viralicidic activity 2-like 2 (S. cerevisiae) (SKIV2L2) 10 ug
$789.00 MSRP $1,107.00 MSRP $1,107.00
SC100442 SKIV2L2 (untagged)-Human superkiller viralicidic activity 2-like 2 (S. cerevisiae) (SKIV2L2) 10 ug
$908.00

Citations

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