IGF2BP2 (NM_006548) Human Tagged ORF Clone

CAT#: RC205673

IGF2BP2 (Myc-DDK-tagged)-Human insulin-like growth factor 2 mRNA binding protein 2 (IGF2BP2), transcript variant 1

Product Images

Circular map for RC205673

• 

  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY IGF2BP2 (Cat# RC205673, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IGF2BP2(Cat# TA501269). Positive lysates LY401961 (100ug) and LC401961 (20ug) can be purchased separately from OriGene.

• 

  Western blot validation of overexpression lysate (Cat# LY401961) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205673 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified IGF2BP2 protein (Cat# TP305673). The protein was produced from HEK293T cells transfected with IGF2BP2 cDNA clone (Cat# RC205673) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: IGF2BP2
• Synonyms: IMP-2; IMP2; VICKZ2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205673 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACAAGCTTTACATCGGGAACCTGAGCCCCGCCGTCACCGCCGACGACCTCCGGCAGCTCTTTGGGG
  ACAGGAAGCTGCCCCTGGCGGGACAGGTCCTGCTGAAGTCCGGCTACGCCTTCGTGGACTACCCCGACCA
  GAACTGGGCCATCCGCGCCATCGAGACCCTCTCGGGTAAAGTGGAATTGCATGGGAAAATCATGGAAGTT
  GATTACTCAGTCTCTAAAAAGCTAAGGAGCAGGAAAATTCAGATTCGAAACATCCCTCCTCACCTGCAGT
  GGGAGGTGTTGGATGGACTTTTGGCTCAATATGGGACAGTGGAGAATGTGGAACAAGTCAACACAGACAC
  AGAAACCGCCGTTGTCAACGTCACATATGCAACAAGAGAAGAAGCAAAAATAGCCATGGAGAAGCTAAGC
  GGGCATCAGTTTGAGAACTACTCCTTCAAGATTTCCTACATCCCGGATGAAGAGGTGAGCTCCCCTTCGC
  CCCCTCAGCGAGCCCAGCGTGGGGACCACTCTTCCCGGGAGCAAGGCCACGCCCCTGGGGGCACTTCTCA
  GGCCAGACAGATTGATTTCCCGCTGCGGATCCTGGTCCCCACCCAGTTTGTTGGTGCCATCATCGGAAAG
  GAGGGCTTGACCATAAAGAACATCACTAAGCAGACCCAGTCCCGGGTAGATATCCATAGAAAAGAGAACT
  CTGGAGCTGCAGAGAAGCCTGTCACCATCCATGCCACCCCAGAGGGGACTTCTGAAGCATGCCGCATGAT
  TCTTGAAATCATGCAGAAAGAGGCAGATGAGACCAAACTAGCCGAAGAGATTCCTCTGAAAATCTTGGCA
  CACAATGGCTTGGTTGGAAGACTGATTGGAAAAGAAGGCAGAAATTTGAAGAAAATTGAACATGAAACAG
  GGACCAAGATAACAATCTCATCTTTGCAGGATTTGAGCATATACAACCCGGAAAGAACCATCACTGTGAA
  GGGCACAGTTGAGGCCTGTGCCAGTGCTGAGATAGAGATTATGAAGAAGCTGCGTGAGGCCTTTGAAAAT
  GATATGCTGGCTGTTAACCAACAAGCCAATCTGATCCCAGGGTTGAACCTCAGCGCACTTGGCATCTTTT
  CAACAGGACTGTCCGTGCTATCTCCACCAGCAGGGCCCCGCGGAGCTCCCCCCGCTGCCCCCTACCACCC
  CTTCACTACCCACTCCGGATACTTCTCCAGCCTGTACCCCCATCACCAGTTTGGCCCGTTCCCGCATCAT
  CACTCTTATCCAGAGCAGGAGATTGTGAATCTCTTCATCCCAACCCAGGCTGTGGGCGCCATCATCGGGA
  AGAAGGGGGCACACATCAAACAGCTGGCGAGATTCGCCGGAGCCTCTATCAAGATTGCCCCTGCGGAAGG
  CCCAGACGTCAGCGAAAGGATGGTCATCATCACCGGGCCACCGGAAGCCCAGTTCAAGGCCCAGGGACGG
  ATCTTTGGGAAACTGAAAGAGGAAAACTTCTTTAACCCCAAAGAAGAAGTGAAGCTGGAAGCGCATATCA
  GAGTGCCCTCTTCCACAGCTGGCCGGGTGATTGGCAAAGGTGGCAAGACCGTGAACGAACTGCAGAACTT
  AACCAGTGCAGAAGTCATCGTGCCTCGTGACCAAACGCCAGATGAAAATGAGGAAGTGATCGTCAGAATT
  ATCGGGCACTTCTTTGCTAGCCAGACTGCACAGCGCAAGATCAGGGAAATTGTACAACAGGTGAAGCAGC
  AGGAGCAGAAATACCCTCAGGGAGTCGCCTCACAGCGCAGCAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205673 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MNKLYIGNLSPAVTADDLRQLFGDRKLPLAGQVLLKSGYAFVDYPDQNWAIRAIETLSGKVELHGKIMEV
  DYSVSKKLRSRKIQIRNIPPHLQWEVLDGLLAQYGTVENVEQVNTDTETAVVNVTYATREEAKIAMEKLS
  GHQFENYSFKISYIPDEEVSSPSPPQRAQRGDHSSREQGHAPGGTSQARQIDFPLRILVPTQFVGAIIGK
  EGLTIKNITKQTQSRVDIHRKENSGAAEKPVTIHATPEGTSEACRMILEIMQKEADETKLAEEIPLKILA
  HNGLVGRLIGKEGRNLKKIEHETGTKITISSLQDLSIYNPERTITVKGTVEACASAEIEIMKKLREAFEN
  DMLAVNQQANLIPGLNLSALGIFSTGLSVLSPPAGPRGAPPAAPYHPFTTHSGYFSSLYPHHQFGPFPHH
  HSYPEQEIVNLFIPTQAVGAIIGKKGAHIKQLARFAGASIKIAPAEGPDVSERMVIITGPPEAQFKAQGR
  IFGKLKEENFFNPKEEVKLEAHIRVPSSTAGRVIGKGGKTVNELQNLTSAEVIVPRDQTPDENEEVIVRI
  IGHFFASQTAQRKIREIVQQVKQQEQKYPQGVASQRSK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006548
• ORF Size: 1794 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006548.3
• RefSeq Size: 3676 bp
• RefSeq ORF: 1800 bp
• Locus ID: 10644
• UniProt ID: Q9Y6M1
• Cytogenetics: 3q27.2
• Domains: RRM, KH
• MW: 66 kDa
• Gene Summary: This gene encodes a protein that binds the 5' UTR of insulin-like growth factor 2 (IGF2) mRNA and regulates its translation. It plays an important role in metabolism and variation in this gene is associated with susceptibility to diabetes. Alternative splicing and promoter usage results in multiple transcript variants. Related pseudogenes are found on several chromosomes. [provided by RefSeq, Sep 2016]