IGF2BP2 (NM_006548) Human Tagged ORF Clone

CAT#: RC205673

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IGF2BP2 (Myc-DDK-tagged)-Human insulin-like growth factor 2 mRNA binding protein 2 (IGF2BP2), transcript variant 1



  "NM_006548" in other vectors (5)

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 544.00


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Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol IGF2BP2
Synonyms IMP-2; IMP2; VICKZ2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC205673 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACAAGCTTTACATCGGGAACCTGAGCCCCGCCGTCACCGCCGACGACCTCCGGCAGCTCTTTGGGG
ACAGGAAGCTGCCCCTGGCGGGACAGGTCCTGCTGAAGTCCGGCTACGCCTTCGTGGACTACCCCGACCA
GAACTGGGCCATCCGCGCCATCGAGACCCTCTCGGGTAAAGTGGAATTGCATGGGAAAATCATGGAAGTT
GATTACTCAGTCTCTAAAAAGCTAAGGAGCAGGAAAATTCAGATTCGAAACATCCCTCCTCACCTGCAGT
GGGAGGTGTTGGATGGACTTTTGGCTCAATATGGGACAGTGGAGAATGTGGAACAAGTCAACACAGACAC
AGAAACCGCCGTTGTCAACGTCACATATGCAACAAGAGAAGAAGCAAAAATAGCCATGGAGAAGCTAAGC
GGGCATCAGTTTGAGAACTACTCCTTCAAGATTTCCTACATCCCGGATGAAGAGGTGAGCTCCCCTTCGC
CCCCTCAGCGAGCCCAGCGTGGGGACCACTCTTCCCGGGAGCAAGGCCACGCCCCTGGGGGCACTTCTCA
GGCCAGACAGATTGATTTCCCGCTGCGGATCCTGGTCCCCACCCAGTTTGTTGGTGCCATCATCGGAAAG
GAGGGCTTGACCATAAAGAACATCACTAAGCAGACCCAGTCCCGGGTAGATATCCATAGAAAAGAGAACT
CTGGAGCTGCAGAGAAGCCTGTCACCATCCATGCCACCCCAGAGGGGACTTCTGAAGCATGCCGCATGAT
TCTTGAAATCATGCAGAAAGAGGCAGATGAGACCAAACTAGCCGAAGAGATTCCTCTGAAAATCTTGGCA
CACAATGGCTTGGTTGGAAGACTGATTGGAAAAGAAGGCAGAAATTTGAAGAAAATTGAACATGAAACAG
GGACCAAGATAACAATCTCATCTTTGCAGGATTTGAGCATATACAACCCGGAAAGAACCATCACTGTGAA
GGGCACAGTTGAGGCCTGTGCCAGTGCTGAGATAGAGATTATGAAGAAGCTGCGTGAGGCCTTTGAAAAT
GATATGCTGGCTGTTAACCAACAAGCCAATCTGATCCCAGGGTTGAACCTCAGCGCACTTGGCATCTTTT
CAACAGGACTGTCCGTGCTATCTCCACCAGCAGGGCCCCGCGGAGCTCCCCCCGCTGCCCCCTACCACCC
CTTCACTACCCACTCCGGATACTTCTCCAGCCTGTACCCCCATCACCAGTTTGGCCCGTTCCCGCATCAT
CACTCTTATCCAGAGCAGGAGATTGTGAATCTCTTCATCCCAACCCAGGCTGTGGGCGCCATCATCGGGA
AGAAGGGGGCACACATCAAACAGCTGGCGAGATTCGCCGGAGCCTCTATCAAGATTGCCCCTGCGGAAGG
CCCAGACGTCAGCGAAAGGATGGTCATCATCACCGGGCCACCGGAAGCCCAGTTCAAGGCCCAGGGACGG
ATCTTTGGGAAACTGAAAGAGGAAAACTTCTTTAACCCCAAAGAAGAAGTGAAGCTGGAAGCGCATATCA
GAGTGCCCTCTTCCACAGCTGGCCGGGTGATTGGCAAAGGTGGCAAGACCGTGAACGAACTGCAGAACTT
AACCAGTGCAGAAGTCATCGTGCCTCGTGACCAAACGCCAGATGAAAATGAGGAAGTGATCGTCAGAATT
ATCGGGCACTTCTTTGCTAGCCAGACTGCACAGCGCAAGATCAGGGAAATTGTACAACAGGTGAAGCAGC
AGGAGCAGAAATACCCTCAGGGAGTCGCCTCACAGCGCAGCAAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC205673 protein sequence
Red=Cloning site Green=Tags(s)

MNKLYIGNLSPAVTADDLRQLFGDRKLPLAGQVLLKSGYAFVDYPDQNWAIRAIETLSGKVELHGKIMEV
DYSVSKKLRSRKIQIRNIPPHLQWEVLDGLLAQYGTVENVEQVNTDTETAVVNVTYATREEAKIAMEKLS
GHQFENYSFKISYIPDEEVSSPSPPQRAQRGDHSSREQGHAPGGTSQARQIDFPLRILVPTQFVGAIIGK
EGLTIKNITKQTQSRVDIHRKENSGAAEKPVTIHATPEGTSEACRMILEIMQKEADETKLAEEIPLKILA
HNGLVGRLIGKEGRNLKKIEHETGTKITISSLQDLSIYNPERTITVKGTVEACASAEIEIMKKLREAFEN
DMLAVNQQANLIPGLNLSALGIFSTGLSVLSPPAGPRGAPPAAPYHPFTTHSGYFSSLYPHHQFGPFPHH
HSYPEQEIVNLFIPTQAVGAIIGKKGAHIKQLARFAGASIKIAPAEGPDVSERMVIITGPPEAQFKAQGR
IFGKLKEENFFNPKEEVKLEAHIRVPSSTAGRVIGKGGKTVNELQNLTSAEVIVPRDQTPDENEEVIVRI
IGHFFASQTAQRKIREIVQQVKQQEQKYPQGVASQRSK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_006548
ORF Size 1794 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_006548.3
RefSeq Size 3676 bp
RefSeq ORF 1800 bp
Locus ID 10644
UniProt ID Q9Y6M1
Cytogenetics 3q27.2
Domains RRM, KH
MW 66 kDa
Gene Summary This gene encodes a protein that binds the 5' UTR of insulin-like growth factor 2 (IGF2) mRNA and regulates its translation. It plays an important role in metabolism and variation in this gene is associated with susceptibility to diabetes. Alternative splicing and promoter usage results in multiple transcript variants. Related pseudogenes are found on several chromosomes. [provided by RefSeq, Sep 2016]

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