SIRT7 (NM_016538) Human Tagged ORF Clone

CAT#: RC205658

SIRT7 (Myc-DDK-tagged)-Human sirtuin 7 (SIRT7)

Product Images

Western blot validation of overexpression lysate (Cat# LY402562) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205658 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified SIRT7 protein (Cat# TP305658). The protein was produced from HEK293T cells transfected with SIRT7 cDNA clone (Cat# RC205658) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SIRT7
• Synonyms: SIR2L7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205658 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCCGGGGGTCTGAGCCGCTCCGAGCGCAAAGCGGCGGAGCGGGTCCGGAGGTTGCGGGAGGAGC
  AGCAGAGGGAGCGCCTCCGCCAGGTGTCGCGCATCCTGAGGAAGGCGGCGGCGGAGCGCAGCGCCGAGGA
  GGGCCGGCTGCTGGCCGAGAGCGCGGACCTGGTAACGGAGCTGCAGGGCCGGAGCCGGCGGCGCGAGGGC
  CTGAAGCGGCGGCAGGAGGAGGTGTGCGACGACCCGGAGGAGCTGCGGGGGAAGGTCCGGGAGCTGGCCA
  GCGCCGTCCGGAACGCCAAATACTTGGTCGTCTACACAGGCGCGGGAATCAGCACGGCAGCGTCTATCCC
  AGACTACCGGGGCCCTAATGGAGTGTGGACACTGCTTCAGAAAGGGAGAAGCGTTAGTGCTGCCGACCTG
  AGCGAGGCCGAGCCAACCCTCACCCACATGAGCATCACCCGTCTGCATGAGCAGAAGCTGGTGCAGCATG
  TGGTGTCTCAGAACTGTGACGGGCTCCACCTGAGGAGTGGGCTGCCGCGCACGGCCATCTCCGAGCTCCA
  CGGGAACATGTACATTGAAGTCTGTACCTCCTGCGTTCCCAACAGGGAGTACGTGCGGGTGTTCGATGTG
  ACGGAGCGCACTGCCCTCCACAGACACCAGACAGGCCGGACCTGCCACAAGTGTGGGACCCAGCTGCGGG
  ACACCATTGTGCACTTTGGGGAGAGGGGGACGTTGGGGCAGCCTCTGAACTGGGAAGCGGCGACCGAGGC
  TGCCAGCAGAGCAGACACCATCCTGTGTCTAGGGTCCAGCCTGAAGGTTCTAAAGAAGTACCCACGCCTC
  TGGTGCATGACCAAGCCCCCTAGCCGGCGGCCGAAGCTTTACATCGTGAACCTGCAGTGGACCCCGAAGG
  ATGACTGGGCTGCCCTGAAGCTACATGGGAAGTGTGATGACGTCATGCGGCTCCTCATGGCCGAGCTGGG
  CTTGGAGATCCCCGCCTATAGCAGGTGGCAGGATCCCATTTTCTCACTGGCGACTCCCCTGCGTGCTGGT
  GAAGAAGGCAGCCACAGTCGGAAGTCGCTGTGCAGAAGCAGAGAGGAGGCCCCGCCTGGGGACCGGGGTG
  CACCGCTTAGCTCGGCCCCCATCCTAGGGGGCTGGTTTGGCAGGGGCTGCACAAAACGCACAAAAAGGAA
  GAAAGTGACG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205658 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAGGLSRSERKAAERVRRLREEQQRERLRQVSRILRKAAAERSAEEGRLLAESADLVTELQGRSRRREG
  LKRRQEEVCDDPEELRGKVRELASAVRNAKYLVVYTGAGISTAASIPDYRGPNGVWTLLQKGRSVSAADL
  SEAEPTLTHMSITRLHEQKLVQHVVSQNCDGLHLRSGLPRTAISELHGNMYIEVCTSCVPNREYVRVFDV
  TERTALHRHQTGRTCHKCGTQLRDTIVHFGERGTLGQPLNWEAATEAASRADTILCLGSSLKVLKKYPRL
  WCMTKPPSRRPKLYIVNLQWTPKDDWAALKLHGKCDDVMRLLMAELGLEIPAYSRWQDPIFSLATPLRAG
  EEGSHSRKSLCRSREEAPPGDRGAPLSSAPILGGWFGRGCTKRTKRKKVT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016538
• ORF Size: 1200 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016538.1, NP_057622.1
• RefSeq Size: 1749 bp
• RefSeq ORF: 1203 bp
• Locus ID: 51547
• UniProt ID: Q9NRC8
• Cytogenetics: 17q25.3
• Domains: SIR2
• Protein Families: Druggable Genome, Transcription Factors
• MW: 44.9 kDa
• Gene Summary: This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family. [provided by RefSeq, Jul 2008]