Semaphorin 3c (SEMA3C) (NM_006379) Human Tagged ORF Clone

CAT#: RC205269

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SEMA3C (Myc-DDK-tagged)-Human sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3C (SEMA3C)



  "NM_006379" in other vectors (6)

Reconstitution Protocol

USD 688.00


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Size
    • 10 ug


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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol SEMA3C
Synonyms SEMAE; SemE
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC205269 representing NM_006379
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCATTCCGGACAATTTGCGTGTTGGTTGGAGTATTTATTTGTTCTATCTGTGTGAAAGGATCTTCCC
AGCCCCAAGCAAGAGTTTATTTAACATTTGATGAACTTCGAGAAACCAAGACCTCTGAATACTTCAGCCT
TTCCCACCATCCTTTAGACTACAGGATTTTATTAATGGATGAAGATCAGGACCGGATATATGTGGGAAGC
AAAGATCACATTCTTTCCCTGAATATTAACAATATAAGTCAAGAAGCTTTGAGTGTTTTCTGGCCAGCAT
CTACAATCAAAGTTGAAGAATGCAAAATGGCTGGCAAAGATCCCACACACGGCTGTGGGAACTTTGTCCG
TGTAATTCAGACTTTCAATCGCACACATTTGTATGTCTGTGGGAGTGGCGCTTTCAGTCCTGTCTGTACT
TACTTGAACAGAGGGAGGAGATCAGAGGACCAAGTTTTCATGATTGACTCCAAGTGTGAATCTGGAAAAG
GACGCTGCTCTTTCAACCCCAACGTGAACACGGTGTCTGTTATGATCAATGAGGAGCTTTTCTCTGGAAT
GTATATAGATTTCATGGGGACAGATGCTGCTATTTTTCGAAGTTTAACCAAGAGGAATGCGGTCAGAACT
GATCAACATAATTCCAAATGGCTAAGTGAACCTATGTTTGTAGATGCACATGTCATCCCAGATGGTACTG
ATCCAAATGATGCTAAGGTGTACTTCTTCTTCAAAGAAAAACTGACTGACAATAACAGGAGCACGAAACA
GATTCATTCCATGATTGCTCGAATATGTCCTAATGACACTGGTGGACTGCGTAGCCTTGTCAACAAGTGG
ACCACTTTCTTAAAGGCGAGGCTGGTGTGCTCGGTAACAGATGAAGACGGCCCAGAAACACACTTTGATG
AATTAGAGGATGTGTTTCTGCTGGAAACTGATAACCCGAGGACAACACTAGTGTATGGCATTTTTACAAC
ATCAAGCTCAGTTTTCAAAGGATCAGCCGTGTGTGTGTATCATTTATCTGATATACAGACTGTGTTTAAT
GGGCCTTTTGCCCACAAAGAAGGGCCCAATCATCAGCTGATTTCCTATCAGGGCAGAATTCCATATCCTC
GCCCTGGAACTTGTCCAGGAGGAGCATTTACACCCAATATGCGAACCACCAAGGAGTTCCCAGATGATGT
TGTCACTTTTATTCGGAACCATCCTCTCATGTACAATTCCATCTACCCAATCCACAAAAGGCCTTTGATT
GTTCGTATTGGCACTGACTACAAGTATACAAAGATAGCTGTGGATCGAGTGAACGCTGCTGATGGGAGAT
ACCATGTCCTGTTTCTCGGAACAGATCGGGGTACTGTGCAAAAAGTGGTTGTTCTTCCTACTAACAACTC
TGTCAGTGGCGAGCTCATTCTGGAGGAGCTGGAAGTCTTTAAGAATCATGCTCCTATAACAACAATGAAA
ATTTCATCTAAAAAGCAACAGTTGTATGTGAGTTCCAATGAAGGGGTTTCCCAGGTATCTCTGCACCGCT
GCCACATCTATGGTACAGCCTGTGCTGACTGCTGCCTGGCGCGGGACCCTTATTGCGCCTGGGATGGCCA
TTCCTGTTCCAGATTCTACCCAACTGGGAAACGGAGGAGCCGAAGACAAGATGTGAGACATGGAAACCCA
CTGACTCAATGCAGAGGATTTAATCTAAAAGCATACAGAAATGCAGCTGAAATTGTGCAGTATGGAGTAA
AAAATAACACCACTTTTCTGGAGTGTGCCCCCAAGTCTCCGCAGGCATCTATCAAGTGGCTGTTACAGAA
AGACAAAGACAGGAGGAAAGAGGTTAAGCTGAATGAACGAATAATAGCCACTTCACAGGGACTCCTGATC
CGCTCTGTTCAGGGTTCTGACCAAGGACTTTATCACTGCATTGCTACAGAAAATAGTTTCAAGCAGACCA
TAGCCAAGATCAACTTCAAAGTTTTAGATTCAGAAATGGTGGCTGTTGTGACGGACAAATGGTCCCCGTG
GACCTGGGCCAGCTCTGTGAGGGCTTTACCCTTCCACCCGAAGGACATCATGGGGGCATTCAGCCACTCA
GAAATGCAGATGATTAACCAATACTGCAAAGACACTCGGCAGCAACATCAGCAGGGAGATGAATCACAGA
AAATGAGAGGGGACTATGGCAAGTTAAAGGCCCTCATCAATAGTCGGAAAAGTAGAAACAGGAGGAATCA
GTTGCCAGAGTCA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC205269 representing NM_006379
Red=Cloning site Green=Tags(s)

MAFRTICVLVGVFICSICVKGSSQPQARVYLTFDELRETKTSEYFSLSHHPLDYRILLMDEDQDRIYVGS
KDHILSLNINNISQEALSVFWPASTIKVEECKMAGKDPTHGCGNFVRVIQTFNRTHLYVCGSGAFSPVCT
YLNRGRRSEDQVFMIDSKCESGKGRCSFNPNVNTVSVMINEELFSGMYIDFMGTDAAIFRSLTKRNAVRT
DQHNSKWLSEPMFVDAHVIPDGTDPNDAKVYFFFKEKLTDNNRSTKQIHSMIARICPNDTGGLRSLVNKW
TTFLKARLVCSVTDEDGPETHFDELEDVFLLETDNPRTTLVYGIFTTSSSVFKGSAVCVYHLSDIQTVFN
GPFAHKEGPNHQLISYQGRIPYPRPGTCPGGAFTPNMRTTKEFPDDVVTFIRNHPLMYNSIYPIHKRPLI
VRIGTDYKYTKIAVDRVNAADGRYHVLFLGTDRGTVQKVVVLPTNNSVSGELILEELEVFKNHAPITTMK
ISSKKQQLYVSSNEGVSQVSLHRCHIYGTACADCCLARDPYCAWDGHSCSRFYPTGKRRSRRQDVRHGNP
LTQCRGFNLKAYRNAAEIVQYGVKNNTTFLECAPKSPQASIKWLLQKDKDRRKEVKLNERIIATSQGLLI
RSVQGSDQGLYHCIATENSFKQTIAKINFKVLDSEMVAVVTDKWSPWTWASSVRALPFHPKDIMGAFSHS
EMQMINQYCKDTRQQHQQGDESQKMRGDYGKLKALINSRKSRNRRNQLPES

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_006379
ORF Size 2253 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_006379.5
RefSeq Size 5189 bp
RefSeq ORF 2256 bp
Locus ID 10512
UniProt ID Q99985
Cytogenetics 7q21.11
Domains Sema, IG, PSI
Protein Families Secreted Protein
Protein Pathways Axon guidance
MW 85 kDa
Gene Summary This gene encodes a secreted glycoprotein that belongs to the semaphorin class 3 family of neuronal guidance cues. The encoded protein contains an N-terminal sema domain, integrin and immunoglobulin-like domains, and a C-terminal basic domain. Homodimerization and proteolytic cleavage of the C-terminal propeptide are necessary for the function of the encoded protein. It binds a neuropilin co-receptor before forming a heterotrimeric complex with an associated plexin. An increase in the expression of this gene correlates with an increase in cancer cell invasion and adhesion. Naturally occurring mutations in this gene are associated with Hirschsprung disease. [provided by RefSeq, May 2017]

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