Semaphorin 3c (SEMA3C) (NM_006379) Human Untagged Clone

CAT#: SC116160

SEMA3C (untagged)-Human sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3C (SEMA3C)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Semaphorin 3c
• Synonyms: SEMAE; SemE
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_006379 edited
  GAATTCGGCACGAGACTGCGAAAGGAGCAGGGTTGCGGAGCTAGGGCTCCAGCCTGCGGC
  CGCGCATTCTTGCGTCTGGCCAGCCGCGAGCTCTAAGGGTCGGCCCCGCCCGGTCCGCCC
  CCGCGGCTCCCTGCCAGGCTCTCGCGGGCGCGCTCGGGGTGGGGCCTCGCGGCTGGCGGA
  GATGCGGCCGGGGCTGCGCGGTGGTGATGCGAGCCTGCTGGGCGGCGCGCCGGGGCAGCC
  GGAGCCGCGCGCCGCGGCGCTGTAATCGGACACCAAGAGCGCTCGCCCCCGGCCTCCGGC
  CACTTTCCATTCACTCCGAGGTGCTTGATTGAGCGACGCGGAGAAGAGCTCCGGGTGCCG
  CGGCACTGCAGCGCTGAGATTCCTTTACAAAGAAACTCAGAGGACCGGGAAGAAAGAATT
  TCACCTTTGCGACGTGCTAGAAAATAAGGTCGTCTGGGAAAAGGACTGGAGACACAAGCG
  CATCCAACCCCGGTAGCAAACTGATGACTTTTCCGTGCTGATTTCTTTCAACCTCGGTAT
  TTTCCCTTGGATATTAACTTGCATATCTGAAGAAATGGCATTCCGGACAATTTGCGTGTT
  GGTTGGAGTATTTATTTGTTCTATCTGTGTGAAAGGATCTTCCCAGCCCCAAGCAAGAGT
  TTATTTAACATTTGATGAACTTCGAGAAACCAAGACCTCTGAATACTTCAGCCTTTCCCA
  CCATCCTTTAGACTACAGGATTTTATTAATGGATGAAGATCAGGACCGGATATATGTGGG
  AAGCAAAGATCACATTCTTTCCCTGAATATTAACAATATAAGTCAAGAAGCTTTGAGTGT
  TTTCTGGCCAGCATCTACAATCAAAGTTGAAGAATGCAAAATGGCTGGCAAAGATCCCAC
  ACACGGCTGTGGGAACTTTGTCCGTGTAATTCAGACTTTCAATCGCACACATTTGTATGT
  CTGTGGGAGTGGCGCTTTCAGTCCTGTCTGTACTTACTTGAACAGAGGGAGGAGATCAGA
  GGACCAAGTTTTCATGATTGACTCCAAGTGTGAATCTGGAAAAGGACGCTGCTCTTTCAA
  CCCCAACGTGAACACGGTGTCTGTTATGATCAATGAGGAGCTTTTCTCTGGAATGTATAT
  AGATTTCATGGGGACAGATGCTGCTATTTTTCGAAGTTTAACCAAGAGGAATGCGGTCAG
  AACTGATCAACATAATTCCAAATGGCTAAGTGAACCTATGTTTGTAGATGCACATGTCAT
  CCCAGATGGTACTGATCCAAATGATGCTAAGGTGTACTTCTTCTTCAAAGAAAAACTGAC
  TGACAATAACAGGAGCACGAAACAGATTCATTCCATGATTGCTCGAATATGTCCTAATGA
  CACTGGTGGACTGCGTAGCCTTGTCAACAAGTGGACCACTTTCTTAAAGGCGAGGCTGGT
  GTGCTCGGTAACAGATGAAGACGGCCCAGAAACACACTTTGATGAATTAGAGGATGTGTT
  TCTGCTGGAAACTGATAACCCGAGGACAACACTAGTGTATGGCATTTTTACAACATCAAG
  CTCAGTTTTCAAAGGATCAGCCGTGTGTGTGTATCATTTATCTGATATACAGACTGTGTT
  TAATGGGCCTTTTGCCCACAAAGAAGGGCCCAATCATCAGCTGATTTCCTATCAGGGCAG
  AATTCCATATCCTCGCCCTGGAACTTGTCCAGGAGGAGCATTTACACCCAATATGCGAAC
  CACCAAGGAGTTCCCAGATGATGTTGTCACTTTTATTCGGAACCATCCTCTCATGTACAA
  TTCCATCTACCCAATCCACAAAAGGCCTTTGATTGTTCGTATTGGCACTGACTACAAGTA
  TACAAAGATAGCTGTGGATCGAGTGAACGCTGCTGATGGGAGATACCATGTCCTGTTTCT
  CGGAACAGATCGGGGTACTGTGCAAAAAGTGGTTGTTCTTCCTACTAACAACTCTGTCAG
  TGGCGAGCTCATTCTGGAGGAGCTGGAAGTCTTTAAGAATCATGCTCCTATAACAACAAT
  GAAAATTTCATCTAAAAAGCAACAGTTGTATGTGAGTTCCAATGAAGGGGTTTCCCAGGT
  ATCTCTGCACCGCTGCCACATCTATGGTACAGCCTGTGCTGACTGCTGCCTGGCGCGGGA
  CCCTTATTGCGCCTGGGATGGCCATTCCTGTTCCAGATTCTACCCAACTGGGAAACGGAG
  GAGCCGAAGACAAGATGTGAGACATGGAAACCCACTGACTCAATGCAGAGGATTTAATCT
  AAAAGCATACAGAAATGCAGCTGAAATTGTGCAGTATGGAGTAAAAAATAACACCACTTT
  TCTGGAGTGTGCCCCCAAGTCTCCGCAGGCATCTATCAAGTGGCTGTTACAGAAAGACAA
  AGACAGGAGGAAAGAGGTTAAGCTGAATGAACGAATAATAGCCACTTCACAGGGACTCCT
  GATCCGCTCTGTTCAGGGTTCTGACCAAGGACTTTATCACTGCATTGCTACAGAAAATAG
  TTTCAAGCAGACCATAGCCAAGATCAACTTCAAAGTTTTAGATTCAGAAATGGTGGCTGT
  TGTGACGGACAAATGGTCCCCGTGGACCTGGGCCAGCTCTGTGAGGGCTTTACCCTTCCA
  CCCGAAGGACATCATGGGGGCATTCAGCCACTCAGAAATGCAGATGATTAACCAATACTG
  CAAAGACACTCGGCAGCAACATCAGCAGGGAGATGAATCACAGAAAATGAGAGGGGACTA
  TGGCAAGTTAAAGGCCCTCATCAATAGTCGGAAAAGTAGAAACAGGAGGAATCAGTTGCC
  AGAGTCATAATATTTTCTTATGTGGGTCTTATGCTTCCATTAACAAATGCTCTGTCTTCA
  ATGATCAAATTTTGAGCAAAGAAACTTGTGCTTTACCAAGGGGAATTACTGAAAAAGGTG
  ATTACTCCTGAAGTGAGTTTTACACGAACTGAAATGAGCATGCATTTTCTTGTATGATAG
  TGACTAGCACTAGACATGTCATGGTCCTCATGGTGCATATAAATATATTTAACTTAACCC
  AGATTTTATTTATATCTTTATTCACCTTTTCTTCAAAATCGATATGGTGGCTGCAAAACT
  AGAATTGTTGCATCCCTCAATTGAATGAGGGCCATATCCCTGTGGTATTCCTTTCCTGCT
  TTGGGGCTTTAGAATTCTAATTGTCAGTGATTTTGTATATGAAAACAAGTTCCAAATCCA
  CAGCTTTTACGTAGTAAAAGTCATAAATGCATATGACAGAATGGCTATCAAAAGAAATAG
  AAAAGGAAGACGGCATTTAAAGTTGTATAAAAACACGAGTTATTCATAAAGAGAAAATGA
  TGAGTTTTTATGGTTCCAATGAAATATGTTGGGGTTTTTTTAAGATTGTAAAAATAATCA
  GTTACTGGTATCTGTCACTGACCTTTGTTTCCTTATTCAGGAAGATAAAAATCAGTAACC
  TACCCCATGAAGATATTTGGTGGGAGTTATATCAGTGAAGCAGTTTGGTTTATATTCTTA
  TGTTATCACCTTCCAAACAAAAGCACTTACTTTTTTTGGAAGTTATTTAATTTATTTTAG
  ACTCAAAGAATATAATCTTGCACTACTCAGTTATTACTGTTTGTTCTCTTATTCCCTAGT
  CTGTGTGGCAAATTAAACAATATAAGAAGGAAAAATTTGAAGTATTAGACTTCTAAATAA
  GGGGTGAAATCATCAGAAAGAAAAATCAAAGTAGAAACTACTAATTTTTTAAGAGGAATT
  TATAACAAATATGGCTAGTTTTCAACTTCAGTACTCAAATTCAATGATTCTTCCTTTTAT
  TAAAACCAGTCTCAGATATCATACTGATTTTTAAGTCAACACTATATATTTTATGATCTT
  TTCAGTGTGATGGCAAGGTGCTTGTTATGTCTAGAAAGTAAGAAAACAATATGAGGAGAC
  ATTCTGTCTTTCAAAAGGTAATGGTACATACGTTCACTGGTCTCTAAGTGTAAAAGTAGT
  AAATTTTGTGATGAATAAAATAATTATCTCCTAATTGTATGTTAGAATAATTTTATTAGA
  ATAATTTCATACTGAAATTATTTTCTCCAAATAAAAATTAGATGGAAAAATGTGAAAAAA
  ATTATTCATGCTCTCATATATATTTTAAAAACACTACTTTTGCTTTTTTATTTACCTTTT
  AAGACATTTTCATGCTTCCAGGTAAAAACAGATATTGTACCATGTACCTAATCCAAATAT
  CATATAAACATTTTATTTATAGTTAATAATCTATGATGAAGGTAATTAAAGTAGATTATG
  GCCTTTTTAAGTATTGCAGTCTAAAACTTCAAAAACTAAAATCATTGTCAAAATTAATAT
  GATTATTAATCAGAATATCAGAATATGATTCACTATTTAAACTATGATAAATTATGATAA
  TATATGAGGAGGCCTCGCTATAGCAAAAATAGTTAAAATGCTGACATAACACCAAACTTC
  ATTTTTTAAAAAATCTGTTGTTCCAAATGTGTATAATTTTAAAGTAATTTCTAAAGCAGT
  TTATTATAATGGTTTGCCTGCTTAAAAGGTATAATTAAACTTCTTTTCTCTTCTACATTG
  ACACACAGAAATGTGTCAATGTAAAGCCAAAACCATCTTCTGTGTTTATGGCCAATCTAT
  TCTCAAAGTTAAAAGTAAAATTGTTTCAGAGTCACAGTTCCCTTTATTTCACATAAGCCC
  AAACTGATAGACAGTAACGGTGTTTAGTTTTATACTATATTTGTGCTATTTAATTCTTTC
  TATTTTCACAATTATTAAATTGTGTACACTTTCATTACTTTTAAAAATGTAGAAATTCTT
  CATGAACATAACTCTGCTGAATGTAAAAGAAAATTTTTTTTCAAAAATGCTGTTAATGTA
  TACTACTGGTGGTTGATTGGTTTTATTTTATGTAGCTTGACAATTCAGTGACTTAATATC
  TATTCCATTTGTATTGTACATAAAATTTTCTAGAAATACACTTTTTTCCAAAGTGTAAGT
  TTGTGAATAGATTTTAGCATGATGAAACTGTCATAATGGTGAATGTTCAATCTGTGTAAG
  AAAACAAACTAAATGTAGTTGTCACACTAAAATTTAATTGGATATTGATGAAATCATTGG
  CCTGGCAAAATAAAACATGTTGAATTCCCCAAAAAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006379 unedited
  TAATTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGACTGCGAAAGGA
  GCAGGGTTGCGGAGCTAGGGCTCCAGCCTGCGGCCGCGCATTCTTGCGTCTGGCCAGCCG
  CGAGCTCTAAGGGTCGGCCCCGCCCGGTCCGCCCCCGCGGCTCCCTGCCAGGCTCTCGCG
  GGCGCGCTCGGGGTGGGGCCTCGCGGCTGGCGGAGATGCGGCCGGGGCTGCGCGGTGGTG
  ATGCGAGCCTGCTGGGCGGCGCGCCGGGGCAGCCGGAGCCGCGCGCCGCGGCGCTGTAAT
  CGGACACCAAGAGCGCTCGCCCCCGGCCTCCGGCCACTTTCCATTCACTCCGAGGTGCTT
  GATTGAGCGACGCGGAGAAGAGCTCCGGGTGCCGCGGCACTGCAGCGCTGAGATTCCTTT
  ACAAAGAAACTCAGAGGACCGGGAAGAAAGAATTTCACCTTTGCGACGTGCTAGAAAATA
  AGGTCGTCTGGGAAAAGGACTGGAGACACAAGCGCATCCAACCCCGGTAGCAAACTGATG
  ACTTTTCCGTGCTGATTTCTTTCAACCTCGGTATTTTCCCTTGGATATTAACTTGCATAT
  CTGAAGAAATGGCATTCCGGACAATTTGCGTGTTGGTTGGAGTATTTATTTGTTCTATCT
  GTGTGAAAGGATCTTCCCAGCCCCAAGCAAGAGTTTATTTAACATTTGATGAACTTCGAG
  AAACCAAGACCTCTGAATACTTCAGCCTTTCCCACCATCCTTTAGACTACAGGATTNTAT
  TAATGGATGAAGATCAGGACCGGATATATGTGGGAAGCAAAGATCACNATTCTNTNCCCT
  GATATTAACAATATAAGTCAAGAAGCCTTGAGTGTTNTCTGGCCAGCATCTACAATCAAA
  GTTGAGAATGCAAAATGGCTGGNCAAGATCCCACACACGCTGTGGNAAACTTGTNCCGTG
  TATCAGACTTTCATCGACACATTGNAGTCTGTGGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006379 unedited
  TTGTACGCGGCCGCATCTATAGTCGGTTTTTTTTTTTTTTTTTTTTTTGGGGAATTCAAC
  ATGTTTTATTTTGCCAGGCCAATGATTTCATCAATATCCAATTAAATTTTAGTGTGACAA
  CTACATTTAGTTTGGTTTCTTACACAGATTGAACATTCACCATTATGACAGTTTCATCAT
  GCTAAAATCTATTCACAAACTTACACTTTGGAAAAAAGTGTATTTCTAGAAAATTTTATG
  TACAATACAAATGGAATAGATATTAACCGACTGAATTGACCAGCTCCCTAACATAACACG
  CCTGGACCACCAATTGTTTGCGTTGACAGGGTTGCTGAAAAATAACCCCCTTTTACCTTC
  TACCTCACCCTGCTCTTGACCAACCTCCCCCTTCCCACCCACCCCGCCAGCGCCCCCCCC
  CCTCCCTCTGCCCACTCTTCATGAATTTCGCCTTCCTTCCCATCCTCAAACCTACCCTCA
  GTCCCGCCAATCATTCCATCCACTTGCACTCTTCTGGCCCCCGTCACCCTCCCACCCTTC
  CCCCCCTCCAACTCCCAACATCACGCTCTCCCCCACGCCTACCTCTTCCTGCCCCCTCTC
  ACTTTTTTTTCTTCTCCCTTATCCCCTCCGCTGCTCCCATTCTTCTTTTACATTACTACC
  ATCTACCCCACTAACCCCCTCCCCCCTCCGTCACCCGACCTCCCTCCCCCCCCTCGACCG
  CCCCGGTAATGATCGTCTTTTTTCCCTCATCCAACCACCTCGCCCCCTCTCTCCTTTTCT
  TTTCTCCTCACCCCCACCCTCCCACATCCCACCTCCACCCTCTCCCCTCTCCCTCCACCG
  TTACCTCTTAGCCCCACGCGATGCTCTCTCCCCTATCCTCTACCGACTACCTATCTCTTC
  CTCCACTAATTTCCTCCCGATCGCGCACACCCGAGTATANGAGATTTTATAACTTTATTC
  GATATCCCGCCTCTCCGTCCATCTATTCCCGCCTCCCCCGCCG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006379
• Insert Size: 5150 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006379.2, NP_006370.1
• RefSeq Size: 5189 bp
• RefSeq ORF: 2256 bp
• Locus ID: 10512
• UniProt ID: Q99985
• Cytogenetics: 7q21.11
• Domains: Sema, IG, PSI
• Protein Families: Secreted Protein
• Protein Pathways: Axon guidance
• Gene Summary: This gene encodes a secreted glycoprotein that belongs to the semaphorin class 3 family of neuronal guidance cues. The encoded protein contains an N-terminal sema domain, integrin and immunoglobulin-like domains, and a C-terminal basic domain. Homodimerization and proteolytic cleavage of the C-terminal propeptide are necessary for the function of the encoded protein. It binds a neuropilin co-receptor before forming a heterotrimeric complex with an associated plexin. An increase in the expression of this gene correlates with an increase in cancer cell invasion and adhesion. Naturally occurring mutations in this gene are associated with Hirschsprung disease. [provided by RefSeq, May 2017]
  Transcript Variant: This variant (2) contains an alternate exon in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus, compared to isoform 1.