ARF4 (NM_001660) Human Tagged ORF Clone
ARF4 (Myc-DDK-tagged)-Human ADP-ribosylation factor 4 (ARF4)
|Product Name||ARF4 (NM_001660) Human Tagged ORF Clone|
|Type||Human Tagged ORF Clone|
|E. coli Selection||Kanamycin (25 ug/mL)|
>RC204984 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)
>RC204984 protein sequence
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||540 bp|
|OTI Disclaimer||The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info|
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq||NM_001660.1, NM_001660.2, NM_001660.3, NP_001651.1|
|RefSeq Size||1758 bp|
|RefSeq ORF||543 bp|
|Domains||RAB, SAR, ARF, arf|
|Gene Summary||This gene is a member of the human ARF gene family whose members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 5 ARF proteins and 11 ARF-like proteins and constitute one family of the RAS superfamily. The ARF proteins are categorized as class I, class II and class III; this gene is a class II member. The members of each class share a common gene organization. The ARF4 gene spans approximately 12kb and contains six exons and five introns. This gene is the most divergent member of the human ARFs. Conflicting map positions at 3p14 or 3p21 have been reported for this gene. [provided by RefSeq, Jul 2008]|
|The use of this cDNA Clones has been cited in the following citations:|
Class II ADP-ribosylation Factors Are Required for Efficient Secretion of Dengue Viruses
,Mateusz Kudelko, Jean-Baptiste Brault, Kevin Kwok, Ming Yuan Li, Nathalie Pardigon, J. S. Malik Peiris, Roberto Bruzzone, Philippe Desprès, Béatrice Nal, and Pei Gang Wang,
J. Biol. Chem., Jan 2012; 287: 767 - 777.
|cDNA Clone Resources|
|SC119092||ARF4 (untagged)-Human ADP-ribosylation factor 4 (ARF4)||
|RG204984||ARF4 (GFP-tagged) - Human ADP-ribosylation factor 4 (ARF4)||
|RC204984L1||Lenti ORF clone of Human ADP-ribosylation factor 4 (ARF4), Myc-DDK-tagged||
|RC204984L2||Lenti ORF clone of Human ADP-ribosylation factor 4 (ARF4), mGFP tagged||
|RC204984L3||Lenti ORF clone of Human ADP-ribosylation factor 4 (ARF4), Myc-DDK-tagged||
|RC204984L4||Lenti ORF clone of Human ADP-ribosylation factor 4 (ARF4), mGFP tagged||