ARF4 (NM_001660) Human Untagged Clone

CAT#: SC119092

ARF4 (untagged)-Human ADP-ribosylation factor 4 (ARF4)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ARF4
• Synonyms: ARF2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_001660 edited
  ATGGGCCTCACTATCTCCTCCCTCTTCTCCCGACTATTTGGCAAGAAGCAGATGCGCATT
  TTGATGGTTGGATTGGATGCTGCTGGCAAGACAACCATTCTGTATAAACTGAAGTTAGGG
  GAGATAGTCACCACCATTCCTACCATTGGTTTTAATGTGGAAACAGTAGAATATAAGAAC
  ATTTGTTTCACAGTATGGGATGTTGGTGGTCAAGATAGAATTAGGCCTCTCTGGAAGCAT
  TACTTCCAGAATACCCAGGGTCTTATTTTTGTGGTAGATAGCAACGATCGTGAAAGAATT
  CAGGAAGTAGCAGATGAGCTGCAGAAAATGCTTCTGGTAGATGAATTGAGAGATGCAGTG
  CTGCTACTTTTTGCAAACAAACAGGATTTGCCAAATGCTATGGCCATCAGTGAAATGACA
  GATAAACTAGGGCTTCAGTCTCTTCGTAACAGAACATGGTATGTTCAAGCCACTTGTGCA
  ACACAAGGAACTGGTCTGTATGAAGGACTTGACTGGCTGTCAAATGAGCTTTCAAAACGT
  TAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001660 unedited
  GTATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTTCATCGGC
  ACAGGGGCCACTGAGACGTTTCTGCCTCCCTCTTTCTTCCTCCGCTCTTTCTCTTCCCTC
  TCGTTTAGTTTGCCTGGGAGCTTGAAAGGAGAAAGCACGGGGTCGCCCCAAACCCCTTCT
  GCTTCTGCCCATCACAAGTGCCACTACCGCCATGGGCCTCACTATCTCCTCCCTCTTCTC
  CCGACTATTTGGCAAGAAGCAGATGCGCATTTTGATGGTTGGATTGGATGCTGCTGGCAA
  GACAACCATTCTGTATAAACTGAAGTTAGGGGAGATAGTCACCACCATTCCTACCATTGG
  TTTTAATGTGGAAACAGTAGAATATAAGAACATTTGTTTCACAGTATGGGATGTTGGTGG
  TCAAGATAGAATTAGGCCTCTCTGGAAGCATTACTTCCAGAATACCCAGGGTCTTATTTT
  TGTGGTAGATAGCAACGATCGTGAAAGAATTCAGGAAGTAGCAGATGAGCTGCAGAAAAT
  GCTTCTGGTAGATGAATTGAGAGATGCAGTGCTGCTACTTTTTGCAAACAAACAGGATTT
  GCCAAATGCTATGGCCATCAGTGAAATGACAGATAAACTAGGGCTTCAGTCTCTTCGTAA
  CAGAACATGGTATGTTCAAGCCACTTGTGCAACACAAGGAACTGGTCTGTATGAAGGACT
  TGACTGGCTGTCAAATGAGCTCTCAAAACGTTAAATGAAATGGNATATCTAACCAAGGAC
  ATGTTTGATAAAATGGGCTAGGCTTGTTACACAAAATTAGTTTGTATCTTGGTTATTTAA
  CAGTATCTGGGACTGGTTGGGCAAATATTAAACTATTTTGTTGCCAATATTGTTACCGAG
  TATAAGTTGCTATTTACCATGGGCTTGGTTTAAAGGAATCTTCTTGGGAAAAGTATCCTT
  TTAT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001660 unedited
  CCGCGGCACGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTACGTATTTTATCATTTT
  ATTAGGAATAATTCCAAATGGGCTTATGAAAGAAAATGTATTCATCGAATTTGATGAGTT
  TTCCAAAAACTCTTAATGAAGATATGTCTGCCCAATAAACAAAACATCAGCAGAACTATC
  ATATACTGAGCAAAAAATCAGGCTGATAACAAAAGCAACATGCAACATAAAAAAGACACA
  ATATAAAGGAAGACAATCTGCTGAGTATTAAAAACCATCTAAATATCAATTCTTTGCAAC
  CAGCACAGAACTAATTGGCTATCAAATCCAAACCCCACCAAGGTTAAGTTTGGCTGATGT
  AGGCCAGGAGTCAATGTAGGGGGAAAAATTTCCTCAGTGCAAGAGAGCTGAGTAGTGTTT
  TGGATCTTTCTCTGGCTGGTACAAGCAGTATTAGAAAATCTTTTTGGCAAGGGAGAGAAA
  TAAATACAAATGGAATGCTACATTTTTAAATTAGCAAACTGTCTCACGAATGACCCAAGG
  TATCAGTAAAGTAGCAAGGTGATAACTTTAAAACATTATTTGTTTGGGGCTCAAAAACAC
  TCAAAACATTTATTTAAAGGTTGCACAAGAGCTATGTCCAGGCATTTACGCTTATGGGAA
  GTACAACTACAAGACGATACTTTCTCCCAAGGAGAATTTCTTTAACACCCAGCCCATTGC
  TAAAAAACAACATTTATACTTGGTAAAAAATAATGGGAAAAAAAAAAGTTTAAAATTTTG
  CCCAACCAGTCCCAAAATCTGTTTATAAACAAGAAAAAACTAACCCGGCGCACAAGCCAC
  ACCAATTTATAAACAAGGCCTCGCTTAAAATTCCATTCATTTACCGTTTGAAAGGTCATT
  GAAAGCACGACAGGCCTTCTACAAAACACTTCCTTTGGTTGCCAACCGGTGTTGAACACC
  CCGCGNTCGCTCNCAACAACAGTAANCCTAATTATCGGCTTCTCACTGAGGGCATAAACT
  TGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001660
• Insert Size: 1600 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001660.2, NP_001651.1
• RefSeq Size: 1610 bp
• RefSeq ORF: 543 bp
• Locus ID: 378
• UniProt ID: P18085
• Cytogenetics: 3p14.3
• Domains: RAB, SAR, ARF, arf
• Gene Summary: This gene is a member of the human ARF gene family whose members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 5 ARF proteins and 11 ARF-like proteins and constitute one family of the RAS superfamily. The ARF proteins are categorized as class I, class II and class III; this gene is a class II member. The members of each class share a common gene organization. The ARF4 gene spans approximately 12kb and contains six exons and five introns. This gene is the most divergent member of the human ARFs. Conflicting map positions at 3p14 or 3p21 have been reported for this gene. [provided by RefSeq, Jul 2008]