NT5C (NM_014595) Human Tagged ORF Clone
CAT#: RC204265
NT5C (Myc-DDK-tagged)-Human 5', 3'-nucleotidase, cytosolic (NT5C)
ORF Plasmid: tGFP
Lentiviral Particles: DDK DDK w/ Puro mGFP mGFP w/ Puro
AAV Particle: DDK
"NM_014595" in other vectors (7)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | NT5C |
Synonyms | cdN; DNT; dNT-1; DNT1; HEL74; P5N2; PN-I; PN-II; UMPH2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC204265 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCGGAGCGTGCGCGTGCTGGTGGACATGGACGGCGTCCTGGCCGACTTCGAGGCCGGCCTCCTGC GGGGCTTCCGCCGCCGCTTCCCTGAGGAGCCGCACGTGCCGCTGGAGCAGCGCCGCGGCTTCCTGGCCCG CGAGCAGTACCGCGCCCTGCGGCCCGACCTGGCGGATAAAGTGGCCAGTGTGTACGAAGCCCCGGGCTTT TTCCTGGACCTGGAGCCCATCCCGGGAGCCTTGGACGCTGTGCGGGAGATGAACGACCTACCGGACACGC AGGTCTTCATCTGCACCAGCCCCCTGCTGAAGTACCACCACTGTGTGGGTGAGAAGTACCGCTGGGTGGA GCAGCACCTGGGGCCCCAGTTCGTAGAACGAATTATCCTGACAAGGGACAAGACGGTGGTCTTGGGGGAC CTGCTCATTGATGACAAGGACACAGTTCGAGGCCAGGAGGAGACCCCAAGCTGGGAGCACATCTTGTTCA CCTGCTGCCACAATCGGCACCTGGTCCTGCCCCCGACAAGGAGACGGCTGCTCTCCTGGAGTGACAACTG GAGGGAGATCTTAGATAGCAAGCGCGGAGCTGCGCAGCGGGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC204265 protein sequence
Red=Cloning site Green=Tags(s) MARSVRVLVDMDGVLADFEAGLLRGFRRRFPEEPHVPLEQRRGFLAREQYRALRPDLADKVASVYEAPGF FLDLEPIPGALDAVREMNDLPDTQVFICTSPLLKYHHCVGEKYRWVEQHLGPQFVERIILTRDKTVVLGD LLIDDKDTVRGQEETPSWEHILFTCCHNRHLVLPPTRRRLLSWSDNWREILDSKRGAAQRE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_014595 |
ORF Size | 603 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_014595.3 |
RefSeq Size | 975 bp |
RefSeq ORF | 606 bp |
Locus ID | 30833 |
UniProt ID | Q8TCD5 |
Cytogenetics | 17q25.1 |
Protein Families | Transcription Factors |
Protein Pathways | Metabolic pathways, Nicotinate and nicotinamide metabolism, Purine metabolism, Pyrimidine metabolism |
MW | 23.4 kDa |
Gene Summary | This gene encodes a nucleotidase that catalyzes the dephosphorylation of the 5' deoxyribonucleotides (dNTP) and 2'(3')-dNTP and ribonucleotides, but not 5' ribonucleotides. Of the different forms of nucleotidases characterized, this enzyme is unique in its preference for 5'-dNTP. It may be one of the enzymes involved in regulating the size of dNTP pools in cells. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Nov 2011] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC204265L1 | Lenti ORF clone of Human 5', 3'-nucleotidase, cytosolic (NT5C), Myc-DDK-tagged |
USD 600.00 |
|
RC204265L2 | Lenti ORF clone of Human 5', 3'-nucleotidase, cytosolic (NT5C), mGFP tagged |
USD 600.00 |
|
RC204265L3 | Lenti ORF clone of Human 5', 3'-nucleotidase, cytosolic (NT5C), Myc-DDK-tagged |
USD 600.00 |
|
RC204265L4 | Lenti ORF clone of Human 5', 3'-nucleotidase, cytosolic (NT5C), mGFP tagged |
USD 600.00 |
|
RG204265 | NT5C (tGFP-tagged) - Human 5', 3'-nucleotidase, cytosolic (NT5C) |
USD 500.00 |
|
SC114947 | NT5C (untagged)-Human 5', 3'-nucleotidase, cytosolic (NT5C) |
USD 300.00 |
|
SC320933 | NT5C (untagged)-Human 5', 3'-nucleotidase, cytosolic (NT5C) |
USD 300.00 |
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