C9orf30 (MSANTD3) (NM_080655) Human Tagged ORF Clone

CAT#: RC203850

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MSANTD3 (Myc-DDK-tagged)-Human chromosome 9 open reading frame 30 (C9orf30), transcript variant 1



  "NM_080655" in other vectors (6)

Reconstitution Protocol

USD 300.00


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Size
    • 10 ug


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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol MSANTD3
Synonyms C9orf30; L8
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC203850 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAAAACAACGAAATTATAAAGCCTGCCAAATACTTCTCAGAATTGGAAAAGAGCATCCTGCTGGCTT
TAGTAGAAAAGTATAAATATGTGCTGGAATGTAAGAAAAGTGATGCGCGAACTATTGCCCTTAAGCAGCG
TACCTGGCAGGCGCTGGCCCACGAATACAACTCTCAGCCCAGCGTGTCCCTGCGGGATTTCAAACAGCTG
AAGAAGTGCTGGGAGAACATCAAGGCTCGGACCAAAAAAATTATGGCCCATGAAAGGAGAGAGAAAGTGA
AACGGAGCGTCAGCCCTCTCCTGAGTACCCACGTCCTAGGGAAGGAGAAGATCGCCAGCATGCTGCCGGA
GCAGCTCTACTTCCTGCAGAGCCCCCCGGAGGAGGAGCCCGAATACCACCCCGACGCCTCAGCCCAAGAA
TCATTTGCTGTTTCAAATAGAGAACTGTGCGATGATGAGAAAGAGTTCATACATTTTCCAGTATGTGAGG
GGACCTCTCAACCTGAACCCTCGTGTTCAGCTGTCAGAATAACAGCCAATAAAAACTACAGGAGCAAAAC
CTCTCAGGAAGGTGCTTTAAAAAAGATGCATGAGGAAGAACACCATCAACAAATGTCCATCTTACAACTG
CAACTGATACAAATGAATGAGGTGCATGTGGCCAAAATCCAGCAGATAGAGCGAGAGTGTGAGATGGCAG
AGGAGGAACACAGGATAAAAATGGAAGTTCTCAATAAAAAGAAGATGTATTGGGAAAGAAAACTACAAAC
TTTTACCAAGGAATGGCCTGTTTCCTCATTTAACCGGCCCTTTCCCAATTCGCCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC203850 protein sequence
Red=Cloning site Green=Tags(s)

MQNNEIIKPAKYFSELEKSILLALVEKYKYVLECKKSDARTIALKQRTWQALAHEYNSQPSVSLRDFKQL
KKCWENIKARTKKIMAHERREKVKRSVSPLLSTHVLGKEKIASMLPEQLYFLQSPPEEEPEYHPDASAQE
SFAVSNRELCDDEKEFIHFPVCEGTSQPEPSCSAVRITANKNYRSKTSQEGALKKMHEEEHHQQMSILQL
QLIQMNEVHVAKIQQIERECEMAEEEHRIKMEVLNKKKMYWERKLQTFTKEWPVSSFNRPFPNSP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_080655
ORF Size 828 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_080655.3
RefSeq Size 1826 bp
RefSeq ORF 828 bp
Locus ID 91283
UniProt ID Q96H12
Cytogenetics 9q31.1
MW 32.4 kDa

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