Pleckstrin (PLEK) (NM_002664) Human Tagged ORF Clone

CAT#: RC203780

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PLEK (Myc-DDK-tagged)-Human pleckstrin (PLEK)



  "NM_002664" in other vectors (6)

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 477.00


Availability*
In Stock

Size
    • 10 ug


Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol PLEK
Synonyms P47
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC203780 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAACCAAAGCGGATCAGAGAGGGCTACCTTGTGAAGAAGGGGAGCGTGTTCAATACGTGGAAACCCA
TGTGGGTTGTATTGTTAGAAGATGGAATTGAATTCTATAAGAAGAAAAGTGACAACAGCCCCAAAGGAAT
GATCCCGCTGAAAGGGAGCACTCTGACTAGCCCTTGTCAAGACTTTGGCAAAAGGATGTTTGTGTTTAAG
ATCACTACGACCAAACAGCAGGACCACTTCTTCCAGGCAGCCTTCCTGGAGGAGAGAGATGCCTGGGTTC
GGGATATCAAGAAGGCCATTAAATGCATTGAAGGAGGCCAGAAATTTGCCAGGAAATCTACCAGGAGGTC
CATTCGACTGCCAGAAACCATTGACTTAGGTGCCTTATATTTGTCCATGAAAGACACTGAAAAAGGAATA
AAAGAACTGAATCTAGAGAAGGACAAGAAGATTTTTAATCACTGCTTCACAGGTAACTGCGTCATTGATT
GGCTGGTATCCAACCAGTCTGTTAGGAATCGCCAGGAAGGCCTCATGATTGCTTCATCGCTGCTCAATGA
GGGGTATCTGCAGCCTGCTGGAGACATGTCCAAGAGTGCAGTGGATGGAACTGCTGAAAACCCTTTCCTG
GACAACCCTGATGCCTTCTACTACTTTCCAGACAGTGGGTTCTTCTGTGAAGAGAATTCCAGTGATGATG
ATGTGATTCTGAAAGAAGAATTCAGAGGGGTCATTATCAAGCAGGGATGTTTACTGAAGCAGGGGCATAG
AAGGAAAAACTGGAAAGTGAGGAAGTTCATCTTGAGAGAAGACCCTGCCTACCTGCACTACTATGACCCT
GCTGGGGCAGAAGATCCCCTGGGAGCAATTCACTTGAGAGGCTGTGTGGTGACTTCAGTGGAGAGCAACT
CAAATGGCAGGAAGAGTGAGGAAGAGAACCTTTTTGAGATCATCACAGCAGATGAAGTGCACTATTTCTT
GCAAGCAGCCACCCCCAAGGAGCGCACAGAGTGGATCAAAGCCATCCAGATGGCCTCCCGAACTGGGAAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC203780 protein sequence
Red=Cloning site Green=Tags(s)

MEPKRIREGYLVKKGSVFNTWKPMWVVLLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFGKRMFVFK
ITTTKQQDHFFQAAFLEERDAWVRDIKKAIKCIEGGQKFARKSTRRSIRLPETIDLGALYLSMKDTEKGI
KELNLEKDKKIFNHCFTGNCVIDWLVSNQSVRNRQEGLMIASSLLNEGYLQPAGDMSKSAVDGTAENPFL
DNPDAFYYFPDSGFFCEENSSDDDVILKEEFRGVIIKQGCLLKQGHRRKNWKVRKFILREDPAYLHYYDP
AGAEDPLGAIHLRGCVVTSVESNSNGRKSEEENLFEIITADEVHYFLQAATPKERTEWIKAIQMASRTGK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_002664
ORF Size 1050 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_002664.3
RefSeq Size 2866 bp
RefSeq ORF 1053 bp
Locus ID 5341
UniProt ID P08567
Cytogenetics 2p14
Domains DEP, PH
Protein Families Druggable Genome
MW 40.1 kDa
Gene Summary Major protein kinase C substrate of platelets.[UniProtKB/Swiss-Prot Function]

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