Pleckstrin (PLEK) (NM_002664) Human Tagged ORF Clone

CAT#: RC203780

PLEK (Myc-DDK-tagged)-Human pleckstrin (PLEK)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY PLEK (Cat# RC203780, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PLEK (Cat# TA500902). Positive lysates LY419171 (100ug) and LC419171 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY419171) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203780 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified PLEK protein (Cat# TP303780). The protein was produced from HEK293T cells transfected with PLEK cDNA clone (Cat# RC203780) using MegaTran 2.0 (Cat# TT210002).

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  Anti-PLEK mouse monoclonal antibody (TA500902) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PLEK (RC203780).

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  HEK293T cells transfected with either pCMV6-ENTRY PLEK (RC203780) (Red) or empty vector control plasmid (Blue) were immunostained with anti-PLEK mouse monoclonal (TA500902), and then analyzed by flow cytometry.

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Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PLEK
• Synonyms: P47
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203780 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAACCAAAGCGGATCAGAGAGGGCTACCTTGTGAAGAAGGGGAGCGTGTTCAATACGTGGAAACCCA
  TGTGGGTTGTATTGTTAGAAGATGGAATTGAATTCTATAAGAAGAAAAGTGACAACAGCCCCAAAGGAAT
  GATCCCGCTGAAAGGGAGCACTCTGACTAGCCCTTGTCAAGACTTTGGCAAAAGGATGTTTGTGTTTAAG
  ATCACTACGACCAAACAGCAGGACCACTTCTTCCAGGCAGCCTTCCTGGAGGAGAGAGATGCCTGGGTTC
  GGGATATCAAGAAGGCCATTAAATGCATTGAAGGAGGCCAGAAATTTGCCAGGAAATCTACCAGGAGGTC
  CATTCGACTGCCAGAAACCATTGACTTAGGTGCCTTATATTTGTCCATGAAAGACACTGAAAAAGGAATA
  AAAGAACTGAATCTAGAGAAGGACAAGAAGATTTTTAATCACTGCTTCACAGGTAACTGCGTCATTGATT
  GGCTGGTATCCAACCAGTCTGTTAGGAATCGCCAGGAAGGCCTCATGATTGCTTCATCGCTGCTCAATGA
  GGGGTATCTGCAGCCTGCTGGAGACATGTCCAAGAGTGCAGTGGATGGAACTGCTGAAAACCCTTTCCTG
  GACAACCCTGATGCCTTCTACTACTTTCCAGACAGTGGGTTCTTCTGTGAAGAGAATTCCAGTGATGATG
  ATGTGATTCTGAAAGAAGAATTCAGAGGGGTCATTATCAAGCAGGGATGTTTACTGAAGCAGGGGCATAG
  AAGGAAAAACTGGAAAGTGAGGAAGTTCATCTTGAGAGAAGACCCTGCCTACCTGCACTACTATGACCCT
  GCTGGGGCAGAAGATCCCCTGGGAGCAATTCACTTGAGAGGCTGTGTGGTGACTTCAGTGGAGAGCAACT
  CAAATGGCAGGAAGAGTGAGGAAGAGAACCTTTTTGAGATCATCACAGCAGATGAAGTGCACTATTTCTT
  GCAAGCAGCCACCCCCAAGGAGCGCACAGAGTGGATCAAAGCCATCCAGATGGCCTCCCGAACTGGGAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203780 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEPKRIREGYLVKKGSVFNTWKPMWVVLLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFGKRMFVFK
  ITTTKQQDHFFQAAFLEERDAWVRDIKKAIKCIEGGQKFARKSTRRSIRLPETIDLGALYLSMKDTEKGI
  KELNLEKDKKIFNHCFTGNCVIDWLVSNQSVRNRQEGLMIASSLLNEGYLQPAGDMSKSAVDGTAENPFL
  DNPDAFYYFPDSGFFCEENSSDDDVILKEEFRGVIIKQGCLLKQGHRRKNWKVRKFILREDPAYLHYYDP
  AGAEDPLGAIHLRGCVVTSVESNSNGRKSEEENLFEIITADEVHYFLQAATPKERTEWIKAIQMASRTGK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002664
• ORF Size: 1050 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002664.3
• RefSeq Size: 2866 bp
• RefSeq ORF: 1053 bp
• Locus ID: 5341
• UniProt ID: P08567
• Cytogenetics: 2p14
• Domains: DEP, PH
• Protein Families: Druggable Genome
• MW: 40.1 kDa
• Gene Summary: Major protein kinase C substrate of platelets.[UniProtKB/Swiss-Prot Function]