Pleckstrin (PLEK) (NM_002664) Human Tagged ORF Clone

CAT#: RG203780

PLEK (tGFP-tagged) - Human pleckstrin (PLEK)

Product Images

Circular map for RG203780

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Pleckstrin
• Synonyms: P47
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG203780 representing NM_002664
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAACCAAAGCGGATCAGAGAGGGCTACCTTGTGAAGAAGGGGAGCGTGTTCAATACGTGGAAACCCA
  TGTGGGTTGTATTGTTAGAAGATGGAATTGAATTCTATAAGAAGAAAAGTGACAACAGCCCCAAAGGAAT
  GATCCCGCTGAAAGGGAGCACTCTGACTAGCCCTTGTCAAGACTTTGGCAAAAGGATGTTTGTGTTTAAG
  ATCACTACGACCAAACAGCAGGACCACTTCTTCCAGGCAGCCTTCCTGGAGGAGAGAGATGCCTGGGTTC
  GGGATATCAAGAAGGCCATTAAATGCATTGAAGGAGGCCAGAAATTTGCCAGGAAATCTACCAGGAGGTC
  CATTCGACTGCCAGAAACCATTGACTTAGGTGCCTTATATTTGTCCATGAAAGACACTGAAAAAGGAATA
  AAAGAACTGAATCTAGAGAAGGACAAGAAGATTTTTAATCACTGCTTCACAGGTAACTGCGTCATTGATT
  GGCTGGTATCCAACCAGTCTGTTAGGAATCGCCAGGAAGGCCTCATGATTGCTTCATCGCTGCTCAATGA
  GGGGTATCTGCAGCCTGCTGGAGACATGTCCAAGAGTGCAGTGGATGGAACTGCTGAAAACCCTTTCCTG
  GACAACCCTGATGCCTTCTACTACTTTCCAGACAGTGGGTTCTTCTGTGAAGAGAATTCCAGTGATGATG
  ATGTGATTCTGAAAGAAGAATTCAGAGGGGTCATTATCAAGCAGGGATGTTTACTGAAGCAGGGGCATAG
  AAGGAAAAACTGGAAAGTGAGGAAGTTCATCTTGAGAGAAGACCCTGCCTACCTGCACTACTATGACCCT
  GCTGGGGCAGAAGATCCCCTGGGAGCAATTCACTTGAGAGGCTGTGTGGTGACTTCAGTGGAGAGCAACT
  CAAATGGCAGGAAGAGTGAGGAAGAGAACCTTTTTGAGATCATCACAGCAGATGAAGTGCACTATTTCTT
  GCAAGCAGCCACCCCCAAGGAGCGCACAGAGTGGATCAAAGCCATCCAGATGGCCTCCCGAACTGGGAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG203780 representing NM_002664
  Red=Cloning site Green=Tags(s)
  
  MEPKRIREGYLVKKGSVFNTWKPMWVVLLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFGKRMFVFK
  ITTTKQQDHFFQAAFLEERDAWVRDIKKAIKCIEGGQKFARKSTRRSIRLPETIDLGALYLSMKDTEKGI
  KELNLEKDKKIFNHCFTGNCVIDWLVSNQSVRNRQEGLMIASSLLNEGYLQPAGDMSKSAVDGTAENPFL
  DNPDAFYYFPDSGFFCEENSSDDDVILKEEFRGVIIKQGCLLKQGHRRKNWKVRKFILREDPAYLHYYDP
  AGAEDPLGAIHLRGCVVTSVESNSNGRKSEEENLFEIITADEVHYFLQAATPKERTEWIKAIQMASRTGK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002664
• ORF Size: 1050 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002664.1, NP_002655.1
• RefSeq Size: 2745 bp
• RefSeq ORF: 1053 bp
• Locus ID: 5341
• UniProt ID: P08567
• Cytogenetics: 2p14
• Domains: DEP, PH
• Protein Families: Druggable Genome
• Gene Summary: Major protein kinase C substrate of platelets.[UniProtKB/Swiss-Prot Function]