Pleckstrin (PLEK) (NM_002664) Human Untagged Clone

CAT#: SC108481

PLEK (untagged)-Human pleckstrin (PLEK)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Pleckstrin
• Synonyms: P47
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC108481 sequence for NM_002664 edited (data generated by NextGen Sequencing)
  ATGGAACCAAAGCGGATCAGAGAGGGCTACCTTGTGAAGAAGGGGAGCGTGTTCAATACG
  TGGAAACCCATGTGGGTTGTATTGTTAGAAGATGGAATTGAATTCTATAAGAAGAAAAGT
  GACAACAGCCCCAAAGGAATGATCCCGCTGAAAGGGAGCACTCTGACTAGCCCTTGTCAA
  GACTTTGGCAAAAGGATGTTTGTGTTTAAGATCACTACGACCAAACAGCAGGACCACTTC
  TTCCAGGCAGCCTTCCTGGAGGAGAGAGATGCCTGGGTTCGGGATATCAAGAAGGCCATT
  AAATGCATTGAAGGAGGCCAGAAATTTGCCAGGAAATCTACCAGGAGGTCCATTCGACTG
  CCAGAAACCATTGACTTAGGTGCCTTATATTTGTCCATGAAAGACACTGAAAAAGGAATA
  AAAGAACTGAATCTAGAGAAGGACAAGAAGATTTTTAATCACTGCTTCACAGGTAACTGC
  GTCATTGATTGGCTGGTATCCAACCAGTCTGTTAGGAATCGCCAGGAAGGCCTCATGATT
  GCTTCATCGCTGCTCAATGAGGGGTATCTGCAGCCTGCTGGAGACATGTCCAAGAGTGCA
  GTGGATGGAACTGCTGAAAACCCTTTCCTGGACAACCCTGATGCCTTCTACTACTTTCCA
  GACAGTGGGTTCTTCTGTGAAGAGAATTCCAGTGATGATGATGTGATTCTGAAAGAAGAA
  TTCAGAGGGGTCATTATCAAGCAGGGATGTTTACTGAAGCAGGGGCATAGAAGGAAAAAC
  TGGAAAGTGAGGAAGTTCATCTTGAGAGAAGACCCTGCCTACCTGCACTACTATGACCCT
  GCTGGGGCAGAAGATCCCCTGGGAGCAATTCACTTGAGAGGCTGTGTGGTGACTTCAGTG
  GAGAGCAACTCAAATGGCAGGAAGAGTGAGGAAGAGAACCTTTTTGAGATCATCACAGCA
  GATGAAGTGCACTATTTCTTGCAAGCAGCCACCCCCAAGGAGCGCACAGAGTGGATCAGA
  GCCATCCAGATGGCCTCCCGAACTGGGAAGTAA
  
  Clone variation with respect to NM_002664.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002664 unedited
  CCGTTCGGGATATTTGTATACGACTTACTATAGGCGGCCCGCGAATTCGCACGAGGCCTG
  AGAGTGACCTTTGCATCTGCCTGTCCAGCCAGCATGGAACCAAAGCGGATCAGAGAGGGC
  TACCTTGTGAAGAAGGGGAGCGTGTTCAATACGTGGAAACCCATGTGGGTTGTATTGTTA
  GAAGATGGAATTGAATTCTATAAGAAGAAAAGTGACAACAGCCCCAAAGGAATGATCCCG
  CTGAAAGGGAGCACTCTGACTAGCCCTTGTCAAGACTTTGGCAAAAGGATGTTTGTGTTT
  AAGATCACTACGACCAAACAGCAGGACCACTTCTTCCAGGCAGCCTTCCTGGAGGAGAGA
  GATGCCTGGGTTCGGGATATCAAGAAGGCCATTAAATGCATTGAAGGAGGCCAGAAATTT
  GCCAGGAAATCTACCAGGAGGTCCATTCGACTGCCAGAAACCATTGACTTAGGTGCCTTA
  TATTTGTCCATGAAAGACACTGAAAAAGGAATAAAAGAACTGAATCTAGAGAAGGACAAG
  AAGATTTTTAATCACTGCTTCACAGGTAACTGCGTCATTGATTGGCTGGTATCCAACCAG
  TCTGTTAGGAATCGCCAGGAAGGCCTCATGATTGCTTCATCGCTGCTCAATGAGGGGTAT
  CTGCAGCCTGCTGGAGACATGTCCAAGAGTGCAGTGGATGGAACTGCTGANAACCCTTTC
  CTGGACAACCCTGATGCCTTCTACTACTTTCCAGACAGTGGGTTCTTCTGTGAAGAGAAT
  TCCAGTGATGATGATGTGATTCTGAAAGAAGATTCAGAGGGGTCATTATCAAGCAGGGAT
  GTTTACTGAAGCAGGGGCATAGAAGGAAAAACTGGNNAAGTGAGCAGCTCATCTTGAGAG
  AAGACCCTGCCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002664 unedited
  CTAGAGTCGAGTTTTTTTTTTTTTTTTTTAAGCTAGGGGGACCTCAGGAATCTACTAGTT
  GAATTTCTTCATTTTCCAAAAGAGGCCCTGAGGCCTGGAGAGGGCAAGCAGCCTACTGAG
  GCTGCACAGCTTGTTATATCTGCTTGGGGGTCATGGATAGGGGCAGAGGGGCTGCAGTGA
  GCAATACAATGCAGGGGAACCTTGCACACCACATGGTGAGTTACATTCAGACCCCCCTGC
  AAATGAAAACTTCCCACCCCTGGGCTGTTTCCCGTTGATTTGTCACAGAAGTGGACAGGT
  CCTGGACTGAGCTTGTCCATGGGCTTCCCTCAGGAGGGGAGGAGGAATGCAGGAGTCTCT
  TTACTTCCCAGTCCGGGAGGCCATCTGGATGGCTCTGATCCACTCTGTGCGCTCCTTGGG
  GGTGGCTGCTTGCAAGAAATAGTGCACTTTATCTGCTGTGATGATCTCCAAAAGGTTCTC
  TTCCTCACTCTTCCTGCCATTTGAGTTGCTCTCCACTGAAGTCCCACACAGCCTCTAAGT
  GAATCGCTCCCAGGGGATCCTTTGCCCACCATGCTCATCTATTGCATGTACGCCGGGTCT
  TCTTTAAGCAGAACTTCCTCACTTCCAAGCTTTCCTTTATGCCCCTGCTTAAAAAACATA
  CCCACTGATAAGACCCCTCTGATTTTCCTTCAGACCCAATCCCCCAACTGGCAATCCCTT
  ACCAATAACCCCTGCTGGAACTTTTTCAGCGTTATGGTTCGCCATAAAGGGTTTCAGCTT
  TCTACCCTGGCCTTTGAAACGTTCACCAGCTTAAATCCTTCTTGACACGACCACTAACAG
  TAGCCTCCCGTGATTCTAAACCCGCCCGATCACCCACCCGTCCGCTCCCCGGCAACCGTA
  AAAAACTTTCGCCTCTCCAACAACCCTTTTCCCCTTCACGTCTTCGGCAAATCCGCGCCA
  TTTCCGCCCTGGTCTATCCGCCCCGCTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002664
• Insert Size: 4510 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002664.1, NP_002655.1
• RefSeq Size: 2745 bp
• RefSeq ORF: 1053 bp
• Locus ID: 5341
• UniProt ID: P08567
• Cytogenetics: 2p14
• Domains: DEP, PH
• Protein Families: Druggable Genome
• Gene Summary: Major protein kinase C substrate of platelets.[UniProtKB/Swiss-Prot Function]