SNF2H (SMARCA5) (NM_003601) Human Tagged ORF Clone

CAT#: RC203775

SMARCA5 (Myc-DDK-tagged)-Human SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 5 (SMARCA5)

Product Images

Western blot validation of overexpression lysate (Cat# LY401193) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203775 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified SMARCA5 protein (Cat# TP303775). The protein was produced from HEK293T cells transfected with SMARCA5 cDNA clone (Cat# RC203775) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SMARCA5
• Synonyms: hISWI; hSNF2H; ISWI; SNF2H; WCRF135
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203775 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGTCCGCGGCCGAGCCTCCGCCACCCCCGCCTCCCGAGAGCGCGCCTTCCAAGCCCGCAGCCTCGA
  TCGCCAGCGGCGGGAGCAACAGCAGCAACAAAGGCGGCCCCGAAGGCGTCGCGGCGCAGGCGGTTGCGTC
  TGCGGCCAGCGCTGGTCCCGCAGACGCCGAGATGGAGGAAATATTTGATGATGCGTCACCTGGAAAGCAA
  AAGGAAATCCAAGAACCAGATCCTACCTATGAAGAAAAAATGCAAACTGACCGGGCAAATAGATTCGAGT
  ATTTATTAAAGCAGACAGAACTTTTTGCACATTTCATTCAACCTGCTGCTCAGAAGACTCCAACTTCACC
  TTTGAAGATGAAACCAGGGCGCCCACGAATAAAAAAAGATGAGAAGCAGAACTTACTATCCGTTGGCGAT
  TACCGACACCGTAGAACAGAGCAAGAGGAGGATGAAGAGCTATTAACAGAAAGCTCCAAAGCAACCAATG
  TTTGCACTCGATTTGAAGACTCTCCATCGTATGTAAAATGGGGTAAACTGAGAGATTATCAGGTCCGAGG
  ATTAAACTGGCTCATTTCTTTGTATGAGAATGGCATCAATGGTATCCTTGCAGATGAAATGGGCCTAGGA
  AAGACTCTTCAAACAATTTCTCTTCTTGGGTACATGAAACATTATAGAAACATTCCTGGGCCTCATATGG
  TTTTGGTTCCTAAGTCTACATTACACAACTGGATGAGTGAATTCAAGAGATGGGTACCAACACTTAGATC
  TGTTTGTTTGATAGGAGATAAAGAACAAAGAGCTGCTTTTGTCAGAGACGTTTTATTACCGGGAGAATGG
  GATGTATGTGTAACATCTTATGAAATGCTTATTAAAGAGAAGTCTGTGTTCAAAAAATTTAATTGGAGAT
  ACTTAGTAATAGATGAAGCTCACAGGATCAAAAATGAAAAATCTAAGTTGTCAGAAATAGTGAGGGAATT
  CAAGACTACAAATAGACTATTATTAACTGGAACACCTCTTCAGAACAACTTGCATGAGCTGTGGTCACTT
  CTTAACTTTCTGTTGCCAGATGTGTTTAATTCAGCAGATGACTTTGATTCCTGGTTTGATACAAACAACT
  GCCTTGGGGATCAAAAACTAGTTGAGAGGCTTCATATGGTTTTGCGTCCATTCCTCCTTCGTCGAATTAA
  GGCTGATGTTGAAAAGAGTTTGCCTCCAAAGAAGGAAGTAAAAATCTATGTGGGCCTCAGCAAAATGCAA
  AGGGAATGGTATACTCGGATATTAATGAAGGATATAGATATACTCAACTCAGCAGGCAAGATGGACAAAA
  TGAGGTTATTGAACATCCTAATGCAGTTGAGAAAATGTTGTAATCATCCATATCTCTTTGATGGAGCAGA
  ACCTGGTCCACCTTATACAACAGATATGCATCTAGTAACCAACAGTGGCAAAATGGTGGTTTTAGACAAG
  CTGCTCCCTAAGTTAAAAGAACAAGGTTCACGAGTACTAATCTTCAGTCAAATGACAAGGGTATTGGACA
  TTTTGGAAGATTATTGCATGTGGAGAAATTATGAGTACTGCAGGTTGGATGGTCAGACACCCCATGATGA
  GAGACAAGACTCCATCAATGCATACAATGAACCAAACAGCACAAAGTTTGTTTTCATGTTAAGCACGCGT
  GCTGGTGGTCTTGGCATCAATCTTGCGACTGCTGATGTAGTAATTTTGTATGATTCTGATTGGAATCCCC
  AAGTAGATCTTCAGGCTATGGACCGAGCACATAGAATTGGGCAGACTAAGACAGTCAGAGTGTTCCGCTT
  TATAACTGATAACACTGTAGAAGAAAGAATAGTAGAACGTGCTGAGATGAAACTCAGACTGGATTCAATA
  GTCATTCAACAAGGGAGGCTTGTGGATCAGAATCTGAACAAAATTGGGAAAGATGAAATGCTTCAAATGA
  TTAGACATGGAGCAACACATGTGTTTGCTTCAAAGGAAAGTGAGATCACTGATGAAGATATCGATGGTAT
  TTTGGAAAGAGGTGCAAAGAAGACTGCAGAGATGAATGAAAAGCTCTCCAAGATGGGCGAAAGTTCACTT
  AGAAACTTTACAATGGATACAGAGTCAAGTGTTTATAACTTCGAAGGAGAAGACTATAGAGAAAAACAAA
  AGATTGCATTCACAGAGTGGATTGAACCACCTAAACGAGAAAGAAAAGCCAACTATGCCGTTGATGCATA
  TTTCAGGGAAGCTCTTCGTGTTAGTGAACCTAAAGCACCCAAGGCTCCTCGACCTCCAAAACAACCCAAT
  GTTCAGGATTTCCAGTTCTTTCCTCCACGTTTATTTGAATTACTGGAAAAAGAAATTCTGTTTTACAGAA
  AAACTATTGGGTACAAGGTACCTCGAAATCCTGAGCTGCCTAACGCAGCACAGGCACAAAAAGAAGAACA
  GCTTAAAATTGATGAAGCTGAATCCCTTAATGATGAAGAGTTAGAGGAAAAAGAGAAGCTTCTAACACAG
  GGATTTACCAATTGGAATAAGAGAGATTTTAACCAGTTTATCAAAGCTAATGAGAAGTGGGGTCGTGATG
  ATATTGAAAATATAGCAAGAGAAGTAGAAGGCAAAACTCCAGAAGAAGTCATTGAATATTCAGCTGTGTT
  TTGGGAAAGGTGCAACGAGCTCCAGGACATAGAGAAGATTATGGCTCAGATTGAAAGGGGAGAGGCGAGA
  ATTCAAAGAAGAATAAGCATCAAGAAAGCACTTGACACAAAGATTGGACGGTACAAAGCACCTTTTCATC
  AGCTGAGAATATCATATGGTACTAACAAAGGAAAAAACTATACTGAAGAAGAAGATCGTTTTCTGATTTG
  TATGCTTCACAAACTTGGATTTGACAAAGAAAATGTTTATGATGAATTGCGACAGTGTATTCGCAACTCT
  CCTCAGTTCAGATTTGACTGGTTTCTTAAGTCCAGAACTGCAATGGAGCTCCAGAGGAGATGTAATACCT
  TAATTACTTTGATTGAAAGAGAAAACATGGAACTAGAAGAAAAGGAGAAGGCAGAGAAAAAGAAACGAGG
  ACCAAAGCCTTCAACACAGAAACGTAAAATGGATGGCGCACCTGATGGTCGAGGAAGAAAAAAGAAGCTG
  AAACTA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203775 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSSAAEPPPPPPPESAPSKPAASIASGGSNSSNKGGPEGVAAQAVASAASAGPADAEMEEIFDDASPGKQ
  KEIQEPDPTYEEKMQTDRANRFEYLLKQTELFAHFIQPAAQKTPTSPLKMKPGRPRIKKDEKQNLLSVGD
  YRHRRTEQEEDEELLTESSKATNVCTRFEDSPSYVKWGKLRDYQVRGLNWLISLYENGINGILADEMGLG
  KTLQTISLLGYMKHYRNIPGPHMVLVPKSTLHNWMSEFKRWVPTLRSVCLIGDKEQRAAFVRDVLLPGEW
  DVCVTSYEMLIKEKSVFKKFNWRYLVIDEAHRIKNEKSKLSEIVREFKTTNRLLLTGTPLQNNLHELWSL
  LNFLLPDVFNSADDFDSWFDTNNCLGDQKLVERLHMVLRPFLLRRIKADVEKSLPPKKEVKIYVGLSKMQ
  REWYTRILMKDIDILNSAGKMDKMRLLNILMQLRKCCNHPYLFDGAEPGPPYTTDMHLVTNSGKMVVLDK
  LLPKLKEQGSRVLIFSQMTRVLDILEDYCMWRNYEYCRLDGQTPHDERQDSINAYNEPNSTKFVFMLSTR
  AGGLGINLATADVVILYDSDWNPQVDLQAMDRAHRIGQTKTVRVFRFITDNTVEERIVERAEMKLRLDSI
  VIQQGRLVDQNLNKIGKDEMLQMIRHGATHVFASKESEITDEDIDGILERGAKKTAEMNEKLSKMGESSL
  RNFTMDTESSVYNFEGEDYREKQKIAFTEWIEPPKRERKANYAVDAYFREALRVSEPKAPKAPRPPKQPN
  VQDFQFFPPRLFELLEKEILFYRKTIGYKVPRNPELPNAAQAQKEEQLKIDEAESLNDEELEEKEKLLTQ
  GFTNWNKRDFNQFIKANEKWGRDDIENIAREVEGKTPEEVIEYSAVFWERCNELQDIEKIMAQIERGEAR
  IQRRISIKKALDTKIGRYKAPFHQLRISYGTNKGKNYTEEEDRFLICMLHKLGFDKENVYDELRQCIRNS
  PQFRFDWFLKSRTAMELQRRCNTLITLIERENMELEEKEKAEKKKRGPKPSTQKRKMDGAPDGRGRKKKL
  KL
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003601
• ORF Size: 3156 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003601.3
• RefSeq Size: 7926 bp
• RefSeq ORF: 3159 bp
• Locus ID: 8467
• UniProt ID: O60264
• Cytogenetics: 4q31.21
• Domains: SNF2_N, myb_DNA-binding, DEAD, helicase_C
• Protein Families: Transcription Factors
• MW: 121.9 kDa
• Gene Summary: The protein encoded by this gene is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The protein encoded by this gene is a component of the chromatin remodeling and spacing factor RSF, a facilitator of the transcription of class II genes by RNA polymerase II. The encoded protein is similar in sequence to the Drosophila ISWI chromatin remodeling protein. [provided by RefSeq, Jul 2008]