FKBP10 (NM_021939) Human Tagged ORF Clone

CAT#: RC203676

FKBP10 (Myc-DDK-tagged)-Human FK506 binding protein 10, 65 kDa (FKBP10)

Product Images

Circular map for RC203676

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  Western blot validation of overexpression lysate (Cat# LY411871) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203676 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified FKBP10 protein (Cat# TP303676). The protein was produced from HEK293T cells transfected with FKBP10 cDNA clone (Cat# RC203676) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FKBP10
• Synonyms: BRKS1; FKBP65; hFKBP65; OI6; OI11; PPIASE
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203676 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCCCCGCGGGCCCCCCCAGCCACAGCCTCCTCCGGCTCCCCCTGCTGCAGTTGCTGCTACTGGTGG
  TGCAGGCCGTGGGGAGGGGGCTGGGCCGCGCCAGCCCGGCCGGGGGCCCCCTGGAAGATGTGGTCATCGA
  GAGGTACCACATCCCCAGGGCCTGTCCCCGGGAAGTGCAGATGGGGGATTTTGTGCGCTACCACTACAAC
  GGCACTTTTGAAGATGGCAAGAAGTTTGATTCAAGCTATGATCGCAACACCTTGGTGGCCATCGTGGTGG
  GTGTGGGGCGCCTCATCACTGGCATGGACCGAGGCCTCATGGGCATGTGTGTCAACGAGCGGCGACGCCT
  CATTGTGCCTCCCCACCTGGGCTATGGGAGCATCGGCCTGGCGGGGCTCATTCCACCGGATGCCACCCTC
  TACTTCGATGTGGTTCTGCTGGATGTGTGGAACAAGGAAGACACCGTGCAGGTGAGCACATTGCTGCGCC
  CGCCCCACTGCCCCCGCATGGTCCAGGACGGCGACTTTGTCCGCTACCACTACAATGGCACCCTGCTGGA
  CGGCACCTCCTTCGACACCAGCTACAGTAAGGGCGGCACTTATGACACCTACGTCGGCTCTGGTTGGCTG
  ATCAAGGGCATGGACCAGGGGCTGCTGGGCATGTGTCCTGGAGAGAGAAGGAAGATTATCATCCCTCCAT
  TCCTGGCCTATGGCGAGAAAGGCTATGGGACAGTGATCCCCCCACAGGCCTCGCTGGTCTTTCACGTCCT
  CCTGATTGACGTGCACAACCCGAAGGACGCTGTCCAGCTAGAGACGCTGGAGCTCCCCCCCGGCTGTGTC
  CGCAGAGCCGGGGCCGGGGACTTCATGCGCTACCACTACAATGGCTCCTTGATGGACGGCACCCTCTTCG
  ATTCCAGCTACTCCCGCAACCACACCTACAATACCTATATCGGGCAGGGTTACATCATCCCCGGGATGGA
  CCAGGGGCTGCAGGGTGCCTGCATGGGGGAACGCCGGAGAATTACCATCCCCCCGCACCTCGCCTATGGG
  GAGAATGGAACTGGAGACAAGATCCCTGGCTCTGCCGTGCTAATCTTCAACGTCCATGTCATTGACTTCC
  ACAACCCTGCGGATGTGGTGGAAATCAGGACACTGTCCCGGCCATCTGAGACCTGCAATGAGACCACCAA
  GCTTGGGGACTTTGTTCGATACCATTACAACTGTTCTTTGCTGGACGGCACCCAGCTGTTCACCTCGCAT
  GACTACGGGGCCCCCCAGGAGGCGACTCTCGGGGCCAACAAGGTGATCGAAGGCCTGGACACGGGCCTGC
  AGGGCATGTGTGTGGGAGAGAGGCGGCAGCTCATCGTGCCCCCGCACCTGGCCCACGGGGAGAGTGGAGC
  CCGGGGAGTCCCAGGCAGTGCTGTGCTGCTGTTTGAGGTGGAGCTGGTGTCCCGGGAGGATGGGCTGCCC
  ACAGGCTACCTGTTTGTGTGGCACAAGGACCCTCCTGCCAACCTGTTTGAAGACATGGACCTCAACAAGG
  ATGGCGAGGTCCCTCCGGAGGAGTTCTCCACCTTCATCAAGGCTCAAGTGAGTGAGGGCAAAGGACGCCT
  CATGCCTGGGCAGGACCCTGAGAAAACCATAGGAGACATGTTCCAGAACCAGGACCGCAACCAGGACGGC
  AAGATCACAGTCGACGAGCTCAAGCTGAAGTCAGATGAGGACGAGGAGCGGGTCCACGAGGAGCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203676 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MFPAGPPSHSLLRLPLLQLLLLVVQAVGRGLGRASPAGGPLEDVVIERYHIPRACPREVQMGDFVRYHYN
  GTFEDGKKFDSSYDRNTLVAIVVGVGRLITGMDRGLMGMCVNERRRLIVPPHLGYGSIGLAGLIPPDATL
  YFDVVLLDVWNKEDTVQVSTLLRPPHCPRMVQDGDFVRYHYNGTLLDGTSFDTSYSKGGTYDTYVGSGWL
  IKGMDQGLLGMCPGERRKIIIPPFLAYGEKGYGTVIPPQASLVFHVLLIDVHNPKDAVQLETLELPPGCV
  RRAGAGDFMRYHYNGSLMDGTLFDSSYSRNHTYNTYIGQGYIIPGMDQGLQGACMGERRRITIPPHLAYG
  ENGTGDKIPGSAVLIFNVHVIDFHNPADVVEIRTLSRPSETCNETTKLGDFVRYHYNCSLLDGTQLFTSH
  DYGAPQEATLGANKVIEGLDTGLQGMCVGERRQLIVPPHLAHGESGARGVPGSAVLLFEVELVSREDGLP
  TGYLFVWHKDPPANLFEDMDLNKDGEVPPEEFSTFIKAQVSEGKGRLMPGQDPEKTIGDMFQNQDRNQDG
  KITVDELKLKSDEDEERVHEEL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021939
• ORF Size: 1746 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021939.4
• RefSeq Size: 2888 bp
• RefSeq ORF: 1749 bp
• Locus ID: 60681
• UniProt ID: Q96AY3
• Cytogenetics: 17q21.2
• Protein Families: Druggable Genome
• MW: 64.2 kDa
• Gene Summary: The protein encoded by this gene belongs to the FKBP-type peptidyl-prolyl cis/trans isomerase (PPIase) family. This protein localizes to the endoplasmic reticulum and acts as a molecular chaperone. Alternatively spliced variants encoding different isoforms have been reported, but their biological validity has not been determined.[provided by RefSeq, Nov 2009]