LASS4 (CERS4) (NM_024552) Human Tagged ORF Clone

CAT#: RC203402

CERS4 (Myc-DDK-tagged)-Human LAG1 homolog, ceramide synthase 4 (LASS4)

Product Images

Western blot validation of overexpression lysate (Cat# LY411226) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203402 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified CERS4 protein (Cat# TP303402). The protein was produced from HEK293T cells transfected with CERS4 cDNA clone (Cat# RC203402) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: LASS4
• Synonyms: LASS4; Trh1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203402 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGTCCAGTTTCAACGAGTGGTTTTGGCAGGACAGGTTCTGGTTACCACCCAATGTCACGTGGACAG
  AGCTAGAAGACCGGGATGGCCGTGTCTACCCCCACCCCCAGGACTTGTTGGCAGCCCTGCCCCTGGCGCT
  GGTCCTCCTGGCCATGCGCCTTGCCTTTGAGAGATTCATTGGCCTGCCCCTGAGCCGGTGGCTGGGTGTG
  AGGGATCAGACCAGGAGGCAAGTGAAGCCCAACGCCACGCTGGAGAAACACTTCCTCACGGAAGGGCACA
  GGCCCAAGGAGCCCCAGCTGTCTCTCCTGGCCGCCCAGTGTGGCCTCACGCTGCAGCAGACCCAGCGATG
  GTTCCGGAGACGCCGGAACCAGGATCGACCCCAGCTGACCAAGAAGTTCTGTGAGGCCAGCTGGAGGTTT
  CTCTTCTACCTGTCCTCCTTCGTGGGCGGCCTCTCGGTCCTGTACCACGAGTCATGGCTGTGGGCACCAG
  TAATGTGCTGGGACAGGTACCCAAACCAGACTCTGAAGCCATCCCTGTACTGGTGGTACCTCTTGGAGCT
  GGGTTTCTACCTCTCACTGCTAATCAGGCTGCCCTTTGATGTCAAGCGCAAGGATTTCAAGGAGCAGGTG
  ATACACCACTTCGTGGCGGTCATCCTGATGACCTTCTCCTACAGTGCCAACCTGCTGCGCATTGGCTCTC
  TGGTGCTGCTGTTACATGATTCCTCTGACTACCTGCTGGAGGCCTGTAAGATGGTCAACTACATGCAGTA
  TCAGCAAGTGTGCGACGCTCTCTTCCTCATCTTCTCCTTTGTCTTCTTCTACACCCGACTGGTCCTCTTT
  CCCACCCAGATCCTCTACACCACATACTACGAGTCCATCAGCAACAGGGGCCCCTTCTTCGGCTACTACT
  TCTTCAACGGGCTTCTGATGTTGCTGCAGCTGCTGCACGTGTTCTGGTCTTGCCTCATTCTGCGCATGCT
  CTATAGCTTCATGAAGAAGGGCCAGATGGAGAAGGACATTCGTAGTGATGTAGAAGAATCAGACTCCAGT
  GAGGAGGTGGCGGCGGCCCAGGAACCTCTGCAGCTAAAGAACGGGGCAGCTGGAGGGCCCAGGCCAGCCC
  CCACTGATGGCCCTCAGAGCCGGGTGGCCGGGCGTCTGACCAACAGGCACACAACAGCCACA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203402 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLSSFNEWFWQDRFWLPPNVTWTELEDRDGRVYPHPQDLLAALPLALVLLAMRLAFERFIGLPLSRWLGV
  RDQTRRQVKPNATLEKHFLTEGHRPKEPQLSLLAAQCGLTLQQTQRWFRRRRNQDRPQLTKKFCEASWRF
  LFYLSSFVGGLSVLYHESWLWAPVMCWDRYPNQTLKPSLYWWYLLELGFYLSLLIRLPFDVKRKDFKEQV
  IHHFVAVILMTFSYSANLLRIGSLVLLLHDSSDYLLEACKMVNYMQYQQVCDALFLIFSFVFFYTRLVLF
  PTQILYTTYYESISNRGPFFGYYFFNGLLMLLQLLHVFWSCLILRMLYSFMKKGQMEKDIRSDVEESDSS
  EEVAAAQEPLQLKNGAAGGPRPAPTDGPQSRVAGRLTNRHTTAT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024552
• ORF Size: 1182 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024552.1, NP_078828.1
• RefSeq Size: 1817 bp
• RefSeq ORF: 1185 bp
• Locus ID: 79603
• UniProt ID: Q9HA82
• Cytogenetics: 19p13.2
• Domains: LAG1, TLC
• Protein Families: Transcription Factors, Transmembrane
• MW: 46.4 kDa
• Gene Summary: May be either a bona fide (dihydro)ceramide synthase or a modulator of its activity. When overexpressed in cells is involved in the production of sphingolipids containing different fatty acid donors (N-linked stearoyl- (C18) or arachidoyl- (C20) ceramides) in a fumonisin B1-independent manner (By similarity).[UniProtKB/Swiss-Prot Function]