LASS4 (CERS4) (NM_024552) Human Untagged Clone

CAT#: SC112175

CERS4 (untagged)-Human LAG1 homolog, ceramide synthase 4 (LASS4)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LASS4
• Synonyms: LASS4; Trh1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC112175 sequence for NM_024552 edited (data generated by NextGen Sequencing)
  ATGCTGTCCAGTTTCAACGAGTGGTTTTGGCAGGACAGGTTCTGGTTACCACCCAATGTC
  ACGTGGACAGAGCTAGAAGACCGGGATGGCCGTGTCTACCCCCACCCCCAGGACTTGTTG
  GCAGCCCTGCCCCTGGCGCTGGTCCTCCTGGCCATGCGCCTTGCCTTTGAGAGATTCATT
  GGCCTGCCCCTGAGCCGGTGGCTGGGTGTGAGGGATCAGACCAGGAGGCAAGTGAAGCCC
  AACGCCACGCTGGAGAAACACTTCCTCACGGAAGGGCACAGGCCCAAGGAGCCCCAGCTG
  TCTCTCCTGGCCGCCCAGTGTGGCCTCACGCTGCAGCAGACCCAGCGATGGTTCCGGAGA
  CGCCGGAACCAGGATCGACCCCAGCTGACCAAGAAGTTCTGTGAGGCCAGCTGGAGGTTT
  CTCTTCTACCTGTCCTCCTTCGTGGGCGGCCTCTCGGTCCTGTACCACGAGTCATGGCTG
  TGGGCACCAGTAATGTGCTGGGACAGGTACCCAAACCAGACTCTGAAGCCATCCCTGTAC
  TGGTGGTACCTCTTGGAGCTGGGTTTCTACCTCTCACTGCTAATCAGGCTGCCCTTTGAT
  GTCAAGCGCAAGGATTTCAAGGAGCAGGTGATACACCACTTCGTGGCGGTCATCCTGATG
  ACCTTCTCCTACAGTGCCAACCTGCTGCGCATTGGCTCTCTGGTGCTGCTGTTACACGAT
  TCCTCTGACTACCTGCTGGAGGCCTGTAAGATGGTCAACTACATGCAGTATCAGCAAGTG
  TGCGACGCTCTCTTCCTCATCTTCTCCTTTGTCTTCTTCTACACCCGACTGGTCCTCTTT
  CCCACCCAGATCCTCTACACCACATACTACGAGTCCATCAGCAACAGGGGCCCCTTCTTC
  GGCTACTACTTCTTCAACGGGCTTCTGATGTTGCTGCAGCTGCTGCACGTGTTCTGGTCT
  TGCCTCATTCTGCGCATGCTCTATAGCTTCATGAAGAAGGGCCAGATGGAGAAGGACATT
  CGTAGTGATGTAGAAGAATCAGACTCCAGTGAGGAGGCGGCGGCGGCCCAGGAACCTCTG
  CAGCTAAAGAACGGGACAGCTGGAGGGCCCAGGCCAGCCCCCACTGATGGCCCTCGGAGC
  CGGGTGGCCGGGCGTCTGACCAACAGGCACACAACAGCCACATAG
  
  Clone variation with respect to NM_024552.2
  1096 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_024552 unedited
  GGTTGTCANAATTTGTATACGACTCCTATAGGCGGCCGCGNAATTCGCACGAGGCGAGGC
  CCGGCCAGGAGCAGAGTCCGGCTGCCTGGGGCGGGCGGCGCGTGTCTGCAGCTGCTCCGG
  GTAGCCCGCTAGGCGCGCCGTCCCCAGCCCCGCCGCCGGCCCTCGGTGCGCCCGGCCGCC
  TGCACCCCCAGGAGCAGCTGCTGTGAATAAACACAGAAGTGGAGCTGGGGGACTGATTAG
  AAGCCTCATTCAGTGCACCTGGGCCCCAGCAGGCCCAGCCAGGCGTGGAGGAAGAGGCAT
  TGAGGACTTTCCTTACCTGTTTTTCCAGCTCACCCACTGCCAGCAGAGCATGTCACGTGC
  TCCCGCCTGAGCCTCTGGCCGCAGGAATCAGGTGACCCCAGGCAGCGGGCCAGGCTGCAG
  GGGCGGGATCTGCTCCGTCACCCAGGCTGGAGTGCAGTGGTGTGATCACGGCTCACTGCA
  GCCTCAAACTCCTGGGCTCAACCAGTCCTCTCACCTCAGCCTCCTGAGTAGCCGGGATTA
  CAGAATGCTGTCCAGTTTCAACGAGTGGTTTTGGCAGGACAGGTTCTGGTTACCACCCAA
  TGTCACGTGGACAGAGCTAGAAGACCGGGATGGCCGTGTCTACCCCCACCCCCAGGACTT
  GTTGGCAGCCCTGCCCCTGGCGCTGGTCCTCCTGGCCATGCGCCTTGCCTTTGAGAGATT
  CATTGGCCTGCCCCTGAGCCGGTGGCTGGGTGTGAGGGATCANACCAGGAGGCAGTGAAG
  CCCACGCCACGCTGGAGAAACACTTNCTCACGGAAGGGCACNAGCCCAAGGAGCCCCAGC
  TGTCTCTCCTGGCCGNCCAGTGTGGCCTCACGCTGCAGCAGACCCAGCG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_024552 unedited
  CGCCACGCTTTCTAGNATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCT
  CCATTTGTTTTAATTGGACCCTTTTCAACCTGGGGCTCCCCCCAACCCCCAAGCCACAGG
  CTGGAGGGGTCTCTGGCCAGCCACAACATCCACCTGCTGGCTGCCAAATCTGTCCAGTTG
  CCCACAGGGAAAAAAGGTGGGTGGGCCCAAAGAAGGGGCTGGAGACAGATCATTAATCTT
  TCCACCCACCCCGGGTGGGGCCCTTCCTGTCTTCAAAAAGGGGGCCCAAGGGCGCCAGTT
  CAGTCACTCCACAAATATTCAAGGCACTGGCGGGGGGGCAACCCCTTACAGCCAGTCCCC
  CCCGGCTATGTGGCTGTTGTGCGCCTGTTGGAAAAACCCCCGGTCACCCGGTTTCGAGGG
  CCATCAATGGGGGCTGGCCCGGGCCCTCCAACTGTAACGCTTCTTAACTGCAAAGGCTTC
  TGGGCCGCCGACCCCTTCTTACTGGAGTCTGATTCTTCTACATCACTACCAATGGCCTTC
  TCCCTTCTGGGCCTTCTTCATGAACCTATACAACCTGCGCCAAATGAGGCAAGACCACAA
  CACCCGCCAGCAGCTGCAGCAACCATTCAGAAGCCCCCGTGAAGAAACACCACCCGCAAA
  AAGGGCCCCCCGTTGCTGATGGACTCCCAATATGCGGCGTAAAGGATCTTGCGTGGGAAA
  GAAGACCATCCCGCTGTTCAACAATCCACTAGTCATCATGGGGTATACANCGCCCCCCCT
  TCCCGAAACTGCAGCCCACCACCCTCCCCAAGGCCCCCCCTCGTCTCCTTAGCACCTATC
  CCACTTCCCTCCAAACCCCTGCCCTCTCTTCTCCCCCCCCTCCCAGCCCCCCTCCCCTCT
  CTATCTATCCCCCTCTCCCTCTCTTCTCCTATCCCCCCTCCCCCCTCCACTACTCTTTCC
  CCCCCCCTATTACG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_024552
• Insert Size: 2050 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024552.1, NP_078828.1
• RefSeq Size: 1774 bp
• RefSeq ORF: 1185 bp
• Locus ID: 79603
• UniProt ID: Q9HA82
• Cytogenetics: 19p13.2
• Domains: LAG1, TLC
• Protein Families: Transcription Factors, Transmembrane
• Gene Summary: May be either a bona fide (dihydro)ceramide synthase or a modulator of its activity. When overexpressed in cells is involved in the production of sphingolipids containing different fatty acid donors (N-linked stearoyl- (C18) or arachidoyl- (C20) ceramides) in a fumonisin B1-independent manner (By similarity).[UniProtKB/Swiss-Prot Function]