CORO1B (NM_020441) Human Tagged ORF Clone

CAT#: RC203023

CORO1B (Myc-DDK-tagged)-Human coronin, actin binding protein, 1B (CORO1B), transcript variant 1

Product Images

Circular map for RC203023

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CORO1B (Cat# RC203023, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CORO1B(Cat# TA501573). Positive lysates LY402787 (100ug) and LC402787 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY402787) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203023 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified CORO1B protein (Cat# TP303023). The protein was produced from HEK293T cells transfected with CORO1B cDNA clone (Cat# RC203023) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CORO1B
• Synonyms: CORONIN-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203023 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCTTCCGCAAAGTGGTCCGGCAGAGCAAATTCCGGCATGTGTTCGGGCAGCCGGTCAAGAACGACC
  AGTGCTATGAGGACATTCGCGTGTCCCGTGTTACCTGGGACAGCACCTTCTGCGCCGTCAACCCCAAGTT
  CCTGGCGGTGATTGTGGAGGCCAGTGGAGGGGGTGCCTTTCTGGTGCTCCCCCTAAGCAAGACGGGCCGC
  ATTGACAAGGCCTACCCGACGGTGTGTGGGCACACGGGACCTGTCCTGGACATCGACTGGTGTCCTCACA
  ACGACGAAGTCATAGCCAGCGGCTCGGAGGACTGCACGGTCATGGTGTGGCAGATCCCAGAGAACGGGCT
  GACCTCCCCGCTGACAGAGCCGGTGGTGGTACTGGAGGGGCACACCAAGCGAGTGGGCATCATCGCCTGG
  CACCCCACGGCCCGAAACGTGCTGCTCAGTGCAGGCTGCGACAACGTGGTACTCATCTGGAATGTGGGCA
  CAGCGGAGGAGCTGTACCGCCTGGACAGCCTGCACCCTGACCTCATCTACAATGTCAGCTGGAACCACAA
  TGGCAGCCTGTTTTGCTCAGCATGCAAGGACAAGAGCGTGCGCATCATCGACCCCCGTCGGGGCACCCTG
  GTGGCAGAGCGGGAGAAGGCTCATGAGGGGGCCCGGCCCATGCGGGCCATCTTCCTGGCAGATGGCAAGG
  TGTTCACCACAGGCTTCAGCCGAATGAGCGAGCGGCAGCTGGCGCTCTGGGACCCAGAAAACCTCGAGGA
  ACCCATGGCCCTGCAGGAACTGGACTCGAGCAACGGGGCCCTGCTGCCCTTCTACGACCCCGACACCAGT
  GTGGTCTACGTCTGCGGCAAGGGTGACTCCAGCATCCGGTACTTTGAGATCACAGAGGAGCCTCCCTACA
  TCCACTTCCTGAACACGTTCACCAGCAAGGAGCCGCAGCGGGGTATGGGCAGCATGCCCAAGCGGGGCCT
  GGAGGTCAGCAAGTGCGAGATCGCCCGGTTCTACAAACTGCATGAGCGCAAGTGTGAGCCCATCGTCATG
  ACTGTGCCAAGAAAGTCGGACCTCTTCCAGGATGATCTGTACCCCGACACAGCCGGGCCCGAGGCAGCCC
  TGGAGGCTGAGGAGTGGGTGAGCGGGCGGGATGCCGACCCGATCCTCATCTCACTGCGGGAGGCCTACGT
  GCCCAGCAAGCAGCGGGACCTGAAGATCAGCCGGCGCAACGTGTTGTCTGACAGCCGGCCCGCCATGGCC
  CCGGGCTCCTCCCACCTAGGGGCCCCCGCCTCCACCACCACTGCTGCTGATGCCACCCCCAGCGGCAGCC
  TGGCCAGAGCCGGGGAGGCTGGGAAGCTGGAGGAGGTGATGCAGGAGCTGCGGGCCCTGAGGGCGCTGGT
  CAAGGAGCAGGGCGACCGCATCTGCCGCCTGGAGGAGCAGCTGGGCCGCATGGAGAACGGGGATGCG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203023 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSFRKVVRQSKFRHVFGQPVKNDQCYEDIRVSRVTWDSTFCAVNPKFLAVIVEASGGGAFLVLPLSKTGR
  IDKAYPTVCGHTGPVLDIDWCPHNDEVIASGSEDCTVMVWQIPENGLTSPLTEPVVVLEGHTKRVGIIAW
  HPTARNVLLSAGCDNVVLIWNVGTAEELYRLDSLHPDLIYNVSWNHNGSLFCSACKDKSVRIIDPRRGTL
  VAEREKAHEGARPMRAIFLADGKVFTTGFSRMSERQLALWDPENLEEPMALQELDSSNGALLPFYDPDTS
  VVYVCGKGDSSIRYFEITEEPPYIHFLNTFTSKEPQRGMGSMPKRGLEVSKCEIARFYKLHERKCEPIVM
  TVPRKSDLFQDDLYPDTAGPEAALEAEEWVSGRDADPILISLREAYVPSKQRDLKISRRNVLSDSRPAMA
  PGSSHLGAPASTTTAADATPSGSLARAGEAGKLEEVMQELRALRALVKEQGDRICRLEEQLGRMENGDA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020441
• ORF Size: 1467 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020441.3
• RefSeq Size: 1927 bp
• RefSeq ORF: 1470 bp
• Locus ID: 57175
• UniProt ID: Q9BR76
• Cytogenetics: 11q13.2
• Domains: WD40
• MW: 54.2 kDa
• Gene Summary: Members of the coronin family, such as CORO1B, are WD repeat-containing actin-binding proteins that regulate cell motility (Cai et al., 2005 [PubMed 16027158]).[supplied by OMIM, Mar 2008]