CORO1B (NM_020441) Human Tagged ORF Clone

CAT#: RC203023

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CORO1B (Myc-DDK-tagged)-Human coronin, actin binding protein, 1B (CORO1B), transcript variant 1



  "NM_020441" in other vectors (6)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 686.00


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Size
    • 10 ug


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Other products for "CORO1B"

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol CORO1B
Synonyms CORONIN-2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC203023 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCTTCCGCAAAGTGGTCCGGCAGAGCAAATTCCGGCATGTGTTCGGGCAGCCGGTCAAGAACGACC
AGTGCTATGAGGACATTCGCGTGTCCCGTGTTACCTGGGACAGCACCTTCTGCGCCGTCAACCCCAAGTT
CCTGGCGGTGATTGTGGAGGCCAGTGGAGGGGGTGCCTTTCTGGTGCTCCCCCTAAGCAAGACGGGCCGC
ATTGACAAGGCCTACCCGACGGTGTGTGGGCACACGGGACCTGTCCTGGACATCGACTGGTGTCCTCACA
ACGACGAAGTCATAGCCAGCGGCTCGGAGGACTGCACGGTCATGGTGTGGCAGATCCCAGAGAACGGGCT
GACCTCCCCGCTGACAGAGCCGGTGGTGGTACTGGAGGGGCACACCAAGCGAGTGGGCATCATCGCCTGG
CACCCCACGGCCCGAAACGTGCTGCTCAGTGCAGGCTGCGACAACGTGGTACTCATCTGGAATGTGGGCA
CAGCGGAGGAGCTGTACCGCCTGGACAGCCTGCACCCTGACCTCATCTACAATGTCAGCTGGAACCACAA
TGGCAGCCTGTTTTGCTCAGCATGCAAGGACAAGAGCGTGCGCATCATCGACCCCCGTCGGGGCACCCTG
GTGGCAGAGCGGGAGAAGGCTCATGAGGGGGCCCGGCCCATGCGGGCCATCTTCCTGGCAGATGGCAAGG
TGTTCACCACAGGCTTCAGCCGAATGAGCGAGCGGCAGCTGGCGCTCTGGGACCCAGAAAACCTCGAGGA
ACCCATGGCCCTGCAGGAACTGGACTCGAGCAACGGGGCCCTGCTGCCCTTCTACGACCCCGACACCAGT
GTGGTCTACGTCTGCGGCAAGGGTGACTCCAGCATCCGGTACTTTGAGATCACAGAGGAGCCTCCCTACA
TCCACTTCCTGAACACGTTCACCAGCAAGGAGCCGCAGCGGGGTATGGGCAGCATGCCCAAGCGGGGCCT
GGAGGTCAGCAAGTGCGAGATCGCCCGGTTCTACAAACTGCATGAGCGCAAGTGTGAGCCCATCGTCATG
ACTGTGCCAAGAAAGTCGGACCTCTTCCAGGATGATCTGTACCCCGACACAGCCGGGCCCGAGGCAGCCC
TGGAGGCTGAGGAGTGGGTGAGCGGGCGGGATGCCGACCCGATCCTCATCTCACTGCGGGAGGCCTACGT
GCCCAGCAAGCAGCGGGACCTGAAGATCAGCCGGCGCAACGTGTTGTCTGACAGCCGGCCCGCCATGGCC
CCGGGCTCCTCCCACCTAGGGGCCCCCGCCTCCACCACCACTGCTGCTGATGCCACCCCCAGCGGCAGCC
TGGCCAGAGCCGGGGAGGCTGGGAAGCTGGAGGAGGTGATGCAGGAGCTGCGGGCCCTGAGGGCGCTGGT
CAAGGAGCAGGGCGACCGCATCTGCCGCCTGGAGGAGCAGCTGGGCCGCATGGAGAACGGGGATGCG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC203023 protein sequence
Red=Cloning site Green=Tags(s)

MSFRKVVRQSKFRHVFGQPVKNDQCYEDIRVSRVTWDSTFCAVNPKFLAVIVEASGGGAFLVLPLSKTGR
IDKAYPTVCGHTGPVLDIDWCPHNDEVIASGSEDCTVMVWQIPENGLTSPLTEPVVVLEGHTKRVGIIAW
HPTARNVLLSAGCDNVVLIWNVGTAEELYRLDSLHPDLIYNVSWNHNGSLFCSACKDKSVRIIDPRRGTL
VAEREKAHEGARPMRAIFLADGKVFTTGFSRMSERQLALWDPENLEEPMALQELDSSNGALLPFYDPDTS
VVYVCGKGDSSIRYFEITEEPPYIHFLNTFTSKEPQRGMGSMPKRGLEVSKCEIARFYKLHERKCEPIVM
TVPRKSDLFQDDLYPDTAGPEAALEAEEWVSGRDADPILISLREAYVPSKQRDLKISRRNVLSDSRPAMA
PGSSHLGAPASTTTAADATPSGSLARAGEAGKLEEVMQELRALRALVKEQGDRICRLEEQLGRMENGDA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_020441
ORF Size 1467 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_020441.3
RefSeq Size 1927 bp
RefSeq ORF 1470 bp
Locus ID 57175
UniProt ID Q9BR76
Cytogenetics 11q13.2
Domains WD40
MW 54.2 kDa
Gene Summary Members of the coronin family, such as CORO1B, are WD repeat-containing actin-binding proteins that regulate cell motility (Cai et al., 2005 [PubMed 16027158]).[supplied by OMIM, Mar 2008]

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