CORO1B (NM_020441) Human Untagged Clone

CAT#: SC320143

CORO1B (untagged)-Human coronin, actin binding protein, 1B (CORO1B), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CORO1B
• Synonyms: CORONIN-2
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_020441.2 CCGGGATCCGGAGCCGCCCGAAGCCGGTGCCGCAGCCCCCTGCGCCCCCGGTGCCCCCGA
  CATGTCCTTCCGCAAAGTGGTCCGGCAGAGCAAATTCCGGCATGTGTTCGGGCAGCCGGT
  CAAGAACGACCAGTGCTATGAGGACATTCGCGTGTCCCGTGTTACCTGGGACAGCACCTT
  CTGCGCCGTCAACCCCAAGTTCCTGGCGGTGATTGTGGAGGCCAGTGGAGGGGGTGCCTT
  TCTGGTGCTCCCCCTAAGCAAGACGGGCCGCATTGACAAGGCCTACCCGACGGTGTGTGG
  GCACACGGGACCTGTCCTGGACATCGACTGGTGTCCTCACAACGACGAAGTCATAGCCAG
  CGGCTCGGAGGACTGCACGGTCATGGTGTGGCAGATCCCAGAGAACGGGCTGACCTCCCC
  GCTGACAGAGCCGGTGGTGGTACTGGAGGGGCACACCAAGCGAGTGGGCATCATCGCCTG
  GCACCCCACGGCCCGAAACGTGCTGCTCAGTGCAGGCTGCGACAACGTGGTACTCATCTG
  GAATGTGGGCACAGCGGAGGAGCTGTACCGCCTGGACAGCCTGCACCCTGACCTCATCTA
  CAATGTCAGCTGGAACCACAATGGCAGCCTGTTTTGCTCAGCATGCAAGGACAAGAGCGT
  GCGCATCATCGACCCCCGTCGGGGCACCCTGGTGGCAGAGCGGGAGAAGGCTCATGAGGG
  GGCCCGGCCCATGCGGGCCATCTTCCTGGCAGATGGCAAGGTGTTCACCACAGGCTTCAG
  CCGAATGAGCGAGCGGCAGCTGGCGCTCTGGGACCCAGAAAACCTCGAGGAACCCATGGC
  CCTGCAGGAACTGGACTCGAGCAACGGGGCCCTGCTGCCCTTCTACGACCCCGACACCAG
  TGTGGTCTACGTCTGCGGCAAGGGTGACTCCAGCATCCGGTACTTTGAGATCACAGAGGA
  GCCTCCCTACATCCACTTCCTGAACACGTTCACCAGCAAGGAGCCGCAGCGGGGTATGGG
  CAGCATGCCCAAGCGGGGCCTGGAGGTCAGCAAGTGCGAGATCGCCCGGTTCTACAAACT
  GCATGAGCGCAAGTGTGAGCCCATCGTCATGACTGTGCCAAGAAAGTCGGACCTCTTCCA
  GGATGATCTGTACCCCGACACAGCCGGGCCCGAGGCAGCCCTGGAGGCTGAGGAGTGGGT
  GAGCGGGCGGGATGCCGACCCGATCCTCATCTCACTGCGGGAGGCCTACGTGCCCAGCAA
  GCAGCGGGACCTGAAGATCAGCCGGCGCAACGTGTTGTCTGACAGCCGGCCCGCCATGGC
  CCCGGGCTCCTCCCACCTAGGGGCCCCCGCCTCCACCACCACTGCTGCTGATGCCACCCC
  CAGCGGCAGCCTGGCCAGAGCCGGGGAGGCTGGGAAGCTGGAGGAGGTGATGCAGGAGCT
  GCGGGCCCTGAGGGCGCTGGTCAAGGAGCAGGGCGACCGCATCTGCCGCCTGGAGGAGCA
  GCTGGGCCGCATGGAGAACGGGGATGCGTAGGGCCACAGCCACACGCCACCTTCATCTCC
  TCCGCCGCCCCCTTCCCACTCAGCTTCTGCCAGCGGGTCGCACCGGGCTAGCTGGCTGCC
  CAGAGCCTCTGAGGCAGCGCAGGGGTCAGTTCCCACCCCCACCCGTCCCAGGCCCAGGCC
  GAAGCCAGCGCCCAGCTTTCCTCACTGTTCCTGTGGAGGATGTCTACGCCCAGGCGAGCT
  CCTCGACCTCTGAGGGACCATCTCCCCGACCACTGCCCAGCCCTCTGCTCCCTCCCCAGA
  GGAGGCGGGAGGGTGGGCTCTATATTTTCATTCCAAATAAAATTCTCTTTCTAAAAGCCA
  AAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_020441
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020441.2, NP_065174.1
• RefSeq Size: 1927 bp
• RefSeq ORF: 1470 bp
• Locus ID: 57175
• UniProt ID: Q9BR76
• Cytogenetics: 11q13.2
• Domains: WD40
• Gene Summary: Members of the coronin family, such as CORO1B, are WD repeat-containing actin-binding proteins that regulate cell motility (Cai et al., 2005 [PubMed 16027158]).[supplied by OMIM, Mar 2008]
  Transcript Variant: This variant (1) differs in the 5' UTR compared to variant 2. Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.