PAAF1 (NM_025155) Human Tagged ORF Clone

CAT#: RC202994

PAAF1 (Myc-DDK-tagged)-Human proteasomal ATPase-associated factor 1 (PAAF1)

Product Images

Circular map for RC202994

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY PAAF1 (Cat# RC202994, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PAAF1(Cat# TA507207). Positive lysates LY410872 (100ug) and LC410872 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY410872) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC202994 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified PAAF1 protein (Cat# TP302994). The protein was produced from HEK293T cells transfected with PAAF1 cDNA clone (Cat# RC202994) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PAAF1
• Synonyms: PAAF; Rpn14; WDR71
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202994 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGCCTTTGAGGATTCAGAGCGACTGGGCGCAAGCCCTCAGGAAGGATGAAGGGGAGGCCTGGC
  TGAGCTGTCATCCCCCAGGGAAACCATCTTTGTATGGCAGCCTGACTTGTCAAGGAATTGGCCTAGATGG
  CATCCCAGAGGTTACAGCTTCAGAAGGATTTACTGTGAATGAAATAAACAAGAAAAGCATTCATATTTCA
  TGTCCAAAGGAAAATGCATCTTCTAAGTTTTTGGCACCATATACTACTTTTTCCAGAATTCATACAAAGA
  GTATAACATGCCTGGACATTTCCAGCAGAGGAGGTCTTGGTGTGTCTTCTAGTACTGACGGGACCATGAA
  AATCTGGCAGGCTTCCAATGGAGAACTCAGGAGAGTATTGGAAGGACATGTGTTTGATGTGAATTGTTGC
  AGGTTTTTCCCATCAGGCCTTGTGGTCCTGAGTGGGGGAATGGATGCCCAGCTGAAGATATGGTCAGCTG
  AAGATGCTAGCTGCGTGGTGACCTTCAAAGGTCACAAAGGAGGTATCCTGGATACAGCCATCGTTGATCG
  GGGGAGGAATGTGGTGTCTGCTTCTCGAGATGGGACAGCACGACTTTGGGATTGTGGGCGCTCAGGCTGC
  TTGGGAGTCCTTGCAGATTGTGGTTCTTCTATCAATGGAGTGGCGGTGGGTGCTGCTGACAACTCCATAA
  ACCTTGGCTCCCCTGAGCAGATGCCCAGTGAACGGGAGGTTGGAACAGAGGCCAAAATGCTGCTCTTGGC
  CCGGGAAGATAAGAAACTTCAGTGCTTGGGACTACAGAGCAGGCAGCTGGTGTTCCTCTTTATTGGCTCA
  GACGCTTTCAACTGCTGTACTTTTCTCTCTGGCTTCTTGCTATTGGCTGGGACTCAAGATGGAAACATTT
  ATCAGCTGGATGTGAGGAGTCCAAGGGCTCCGGTACAAGTCATCCACAGATCAGGAGCACCAGTTCTATC
  CCTGCTAAGTGTCAGAGATGGATTCATTGCTAGCCAAGGTGATGGAAGCTGTTTTATTGTCCAGCAAGAC
  TTAGACTATGTCACTGAGCTCACTGGGGCTGACTGTGACCCTGTGTACAAGGTAGCCACATGGGAGAAGC
  AGATCTACACATGCTGTCGAGACGGTCTTGTACGACGCTACCAGCTTTCTGACCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202994 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAPLRIQSDWAQALRKDEGEAWLSCHPPGKPSLYGSLTCQGIGLDGIPEVTASEGFTVNEINKKSIHIS
  CPKENASSKFLAPYTTFSRIHTKSITCLDISSRGGLGVSSSTDGTMKIWQASNGELRRVLEGHVFDVNCC
  RFFPSGLVVLSGGMDAQLKIWSAEDASCVVTFKGHKGGILDTAIVDRGRNVVSASRDGTARLWDCGRSGC
  LGVLADCGSSINGVAVGAADNSINLGSPEQMPSEREVGTEAKMLLLAREDKKLQCLGLQSRQLVFLFIGS
  DAFNCCTFLSGFLLLAGTQDGNIYQLDVRSPRAPVQVIHRSGAPVLSLLSVRDGFIASQGDGSCFIVQQD
  LDYVTELTGADCDPVYKVATWEKQIYTCCRDGLVRRYQLSDL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_025155
• ORF Size: 1176 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025155.3
• RefSeq Size: 1626 bp
• RefSeq ORF: 1179 bp
• Locus ID: 80227
• UniProt ID: Q9BRP4
• Cytogenetics: 11q13.4
• Domains: WD40
• MW: 42.2 kDa
• Gene Summary: This gene encodes a WD repeat-containing protein involved in regulation of association of proteasome components. During HIV infection, the encoded protein is thought to promote provirus transcription through recruitment of the 19S regulatory complex. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jun 2012]