Ube1L (UBA7) (NM_003335) Human Tagged ORF Clone

CAT#: RC202883

UBA7 (Myc-DDK-tagged)-Human ubiquitin-like modifier activating enzyme 7 (UBA7)

Product Images

Circular map for RC202883

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY UBA7 (Cat# RC202883, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UBA7(Cat# TA811855). Positive lysates LY418753 (100ug) and LC418753 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY418753) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC202883 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified UBA7 protein (Cat# TP302883). The protein was produced from HEK293T cells transfected with UBA7 cDNA clone (Cat# RC202883) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ube1L
• Synonyms: D8; UBA1B; UBE1L; UBE2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202883 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGCCCTGGACGCTTCGAAGCTACTGGATGAGGAGCTGTATTCAAGACAGCTGTATGTGCTGGGCT
  CACCTGCCATGCAGAGGATTCAGGGAGCCAGGGTCCTGGTGTCAGGCCTGCAGGGCCTGGGGGCCGAGGT
  GGCCAAGAACTTGGTTCTGATGGGTGTGGGCAGCCTCACTCTGCATGATCCCCACCCCACCTGCTGGTCC
  GACCTGGCTGCCCAGTTTCTCCTCTCAGAGCAGGACTTGGAAAGGAGCAGAGCCGAGGCCTCTCAAGAGC
  TCTTGGCTCAGCTCAACAGAGCTGTCCAGGTCGTCGTGCACACGGGTGACATCACTGAGGACCTGCTGTT
  GGACTTCCAGGTGGTGGTGCTGACTGCTGCAAAGCTGGAGGAGCAGCTGAAGGTGGGCACCTTGTGTCAT
  AAGCATGGAGTTTGCTTTCTGGCGGCTGACACCCGGGGCCTCGTGGGGCAGTTGTTCTGTGACTTTGGTG
  AGGACTTCACTGTGCAGGACCCCACAGAGGCAGAACCCCTGACAGCTGCCATCCAGCACATCTCCCAGGG
  CTCCCCTGGCATTCTCACTCTGAGGAAAGGGGCCAATACCCACTACTTCCGTGATGGAGACTTGGTGACT
  TTCTCGGGAATTGAGGGAATGGTTGAGCTCAACGACTGTGATCCCCGGTCTATCCACGTGCGGGAGGATG
  GGTCCCTGGAGATTGGAGACACAACAACTTTCTCTCGGTACTTGCGTGGTGGGGCTATCACTGAAGTCAA
  GAGACCCAAGACTGTGAGACATAAGTCCCTGGACACAGCCCTGCTCCAGCCCCATGTGGTGGCCCAGAGC
  TCCCAGGAAGTTCACCATGCCCACTGCCTGCATCAGGCCTTCTGTGCACTGCACAAGTTCCAGCACCTCC
  ATGGCCGGCCACCCCAGCCCTGGGATCCTGTTGATGCAGAGACTGTGGTGGGCCTGGCCCGGGACCTGGA
  ACCACTGAAGCGGACAGAGGAAGAGCCACTGGAAGAGCCACTGGATGAGGCCCTAGTGCGGACAGTCGCC
  CTAAGCAGTGCAGGTGTCTTGAGCCCTATGGTGGCCATGCTGGGTGCAGTAGCTGCCCAGGAAGTGCTGA
  AGGCAATCTCCAGGAAGTTCATGCCTCTGGACCAGTGGCTTTACTTTGATGCCCTCGATTGTCTTCCGGA
  AGATGGGGAGCTCCTTCCCAGTCCTGAGGACTGTGCCCTGAGAGGCAGCCGCTATGATGGGCAAATTGCA
  GTGTTTGGGGCTGGTTTTCAGGAGAAACTGAGACGCCAGCACTACCTCCTGGTGGGCGCTGGTGCCATTG
  GTTGTGAGCTGCTCAAAGTCTTTGCCCTAGTGGGACTGGGGGCCGGGAACAGCGGGGGCTTGACTGTTGT
  TGACATGGACCACATAGAGCGCTCCAATCTCAGCCGTCAGTTCCTCTTCAGGTCCCAGGACGTTGGTAGA
  CCCAAGGCAGAGGTGGCTGCAGCAGCTGCCCGGGGCCTGAACCCAGACTTACAGGTGATCCCGCTCACCT
  ACCCACTGGATCCCACCACAGAGCACATCTATGGGGATAACTTTTTCTCCCGTGTGGATGGTGTGGCTGC
  TGCCCTGGACAGTTTCCAGGCCCGGCGCTATGTGGCTGCTCGTTGCACCCACTATCTGAAGCCACTGCTG
  GAGGCAGGCACATCGGGCACCTGGGGCAGTGCTACAGTATTCATGCCACATGTGACTGAGGCCTACAGAG
  CCCCTGCCTCAGCTGCAGCTTCTGAGGATGCCCCCTACCCTGTCTGTACCGTGCGGTACTTCCCTAGCAC
  AGCCGAGCACACCCTGCAGTGGGCCCGGCATGAGTTTGAAGAACTCTTCCGACTGTCTGCAGAGACCATC
  AACCACCACCAACAGGCACACACTTCCCTGGCAGACATGGATGAGCCACAGACACTCACCTTACTGAAGC
  CAGTGCTTGGGGTCCTGAGAGTGCGTCCACAGAACTGGCAAGACTGTGTGGCGTGGGCTCTTGGCCACTG
  GAAACTCTGCTTTCATTATGGCATCAAACAGCTGCTGAGGCACTTCCCACCTAATAAAGTGCTTGAGGAT
  GGAACTCCCTTCTGGTCAGGTCCCAAACAGTGTCCCCAGCCCTTGGAGTTTGACACCAACCAAGACACAC
  ACCTCCTCTACGTACTGGCAGCTGCCAACCTGTATGCCCAGATGCATGGGCTGCCTGGCTCACAGGACTG
  GACTGCACTCAGGGAGCTGCTGAAGCTGCTGCCACAGCCTGACCCCCAACAGATGGCCCCCATCTTTGCT
  AGTAATCTAGAGCTGGCTTCGGCTTCTGCTGAGTTTGGCCCTGAGCAGCAGAAGGAACTGAACAAAGCCC
  TGGAAGTCTGGAGTGTGGGCCCTCCCCTGAAGCCTCTGATGTTTGAGAAGGATGATGACAGCAACTTCCA
  TGTGGACTTTGTGGTAGCGGCAGCTAGCCTGAGATGTCAGAACTACGGGATTCCACCGGTCAACCGTGCC
  CAGAGCAAGCGAATTGTGGGCCAGATTATCCCAGCCATTGCCACCACTACAGCAGCTGTGGCAGGCCTGT
  TGGGCCTGGAGCTGTATAAGGTGGTGAGTGGGCCACGGCCTCGTAGTGCCTTTCGCCACAGCTACCTACA
  TCTGGCTGAAAACTACCTCATCCGCTATATGCCTTTTGCCCCAGCCATCCAGACGTTCCATCACCTGAAG
  TGGACCTCTTGGGACCGTCTGAAGGTACCAGCTGGGCAGCCTGAGAGGACCCTGGAGTCGCTGCTGGCTC
  ATCTTCAGGAGCAGCACGGGTTGAGGGTGAGGATCCTGCTGCACGGCTCAGCCCTGCTCTATGCGGCCGG
  ATGGTCACCTGAAAAGCAGGCCCAGCACCTGCCCCTCAGGGTGACAGAACTGGTTCAGCAGCTGACAGGC
  CAGGCACCTGCTCCTGGGCAGCGGGTGTTGGTGCTAGAGCTGAGCTGTGAGGGTGACGACGAGGACACTG
  CCTTCCCACCTCTGCACTATGAGCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202883 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDALDASKLLDEELYSRQLYVLGSPAMQRIQGARVLVSGLQGLGAEVAKNLVLMGVGSLTLHDPHPTCWS
  DLAAQFLLSEQDLERSRAEASQELLAQLNRAVQVVVHTGDITEDLLLDFQVVVLTAAKLEEQLKVGTLCH
  KHGVCFLAADTRGLVGQLFCDFGEDFTVQDPTEAEPLTAAIQHISQGSPGILTLRKGANTHYFRDGDLVT
  FSGIEGMVELNDCDPRSIHVREDGSLEIGDTTTFSRYLRGGAITEVKRPKTVRHKSLDTALLQPHVVAQS
  SQEVHHAHCLHQAFCALHKFQHLHGRPPQPWDPVDAETVVGLARDLEPLKRTEEEPLEEPLDEALVRTVA
  LSSAGVLSPMVAMLGAVAAQEVLKAISRKFMPLDQWLYFDALDCLPEDGELLPSPEDCALRGSRYDGQIA
  VFGAGFQEKLRRQHYLLVGAGAIGCELLKVFALVGLGAGNSGGLTVVDMDHIERSNLSRQFLFRSQDVGR
  PKAEVAAAAARGLNPDLQVIPLTYPLDPTTEHIYGDNFFSRVDGVAAALDSFQARRYVAARCTHYLKPLL
  EAGTSGTWGSATVFMPHVTEAYRAPASAAASEDAPYPVCTVRYFPSTAEHTLQWARHEFEELFRLSAETI
  NHHQQAHTSLADMDEPQTLTLLKPVLGVLRVRPQNWQDCVAWALGHWKLCFHYGIKQLLRHFPPNKVLED
  GTPFWSGPKQCPQPLEFDTNQDTHLLYVLAAANLYAQMHGLPGSQDWTALRELLKLLPQPDPQQMAPIFA
  SNLELASASAEFGPEQQKELNKALEVWSVGPPLKPLMFEKDDDSNFHVDFVVAAASLRCQNYGIPPVNRA
  QSKRIVGQIIPAIATTTAAVAGLLGLELYKVVSGPRPRSAFRHSYLHLAENYLIRYMPFAPAIQTFHHLK
  WTSWDRLKVPAGQPERTLESLLAHLQEQHGLRVRILLHGSALLYAAGWSPEKQAQHLPLRVTELVQQLTG
  QAPAPGQRVLVLELSCEGDDEDTAFPPLHYEL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003335
• ORF Size: 3036 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: NM_003335.3
• RefSeq Size: 3330 bp
• RefSeq ORF: 3039 bp
• Locus ID: 7318
• UniProt ID: P41226
• Cytogenetics: 3p21.31
• Domains: UBACT, ThiF
• Protein Families: Druggable Genome
• Protein Pathways: Parkinson's disease, Ubiquitin mediated proteolysis
• MW: 111.7 kDa
• Gene Summary: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme is a retinoid target that triggers promyelocytic leukemia (PML)/retinoic acid receptor alpha (RARalpha) degradation and apoptosis in acute promyelocytic leukemia, where it is involved in the conjugation of the ubiquitin-like interferon-stimulated gene 15 protein. [provided by RefSeq, Jul 2008]