C5orf19 (REEP2) (NM_016606) Human Tagged ORF Clone

CAT#: RC202507

REEP2 (Myc-DDK-tagged)-Human receptor accessory protein 2 (REEP2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY REEP2 (Cat# RC202507, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-REEP2(Cat# TA504323). Positive lysates LY413861 (100ug) and LC413861 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY413861) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC202507 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified REEP2 protein (Cat# TP302507). The protein was produced from HEK293T cells transfected with REEP2 cDNA clone (Cat# RC202507) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: REEP2
• Synonyms: C5orf19; SGC32445; SPG72; Yip2d
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202507 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGTCCTGGATCATCTCTCGCCTGGTGGTGCTCATCTTTGGCACCCTGTACCCAGCCTATTCTTCCT
  ACAAGGCCGTGAAGACAAAAAACGTGAAGGAATATGTGAAATGGATGATGTACTGGATCGTCTTTGCCTT
  CTTCACCACGGCCGAGACGCTCACGGATATAGTGCTCTCCTGGTTCCCCTTCTACTTTGAACTGAAGATC
  GCCTTCGTGATATGGCTGCTGTCCCCTTACACCAAGGGCTCCAGCGTGCTCTACCGCAAGTTCGTGCACC
  CAACGCTGTCCAACAAGGAGAAGGAGATCGACGAGTACATCACGCAGGCCCGAGACAAGAGCTATGAGAC
  CATGATGAGGGTGGGCAAGAGGGGCCTGAACCTTGCCGCCAATGCTGCAGTCACAGCTGCCGCCAAGGGG
  GTGCTGTCAGAGAAGCTCCGCAGCTTCAGCATGCAGGACCTGACCCTGATCCGGGACGAGGACGCACTGC
  CCCTGCAGAGGCCTGACGGCCGCCTCCGACCCAGCCCTGGCAGCCTCCTGGACACCATCGAGGACTTAGG
  AGATGACCCTGCCCTGAGTCTAAGGTCCAGCACAAACCCGGCAGATTCCCGGACAGAGGCTTCTGAGGAT
  GACATGGGAGACAAAGCTCCCAAGAGGGCCAAACCCATCAAAAAAGCGCCCAAAGCTGAGCCACTGGCTT
  CCAAGACACTGAAGACCCGGCCCAAGAAGAAGACCTCTGGCGGGGGCGACTCAGCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202507 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVSWIISRLVVLIFGTLYPAYSSYKAVKTKNVKEYVKWMMYWIVFAFFTTAETLTDIVLSWFPFYFELKI
  AFVIWLLSPYTKGSSVLYRKFVHPTLSNKEKEIDEYITQARDKSYETMMRVGKRGLNLAANAAVTAAAKG
  VLSEKLRSFSMQDLTLIRDEDALPLQRPDGRLRPSPGSLLDTIEDLGDDPALSLRSSTNPADSRTEASED
  DMGDKAPKRAKPIKKAPKAEPLASKTLKTRPKKKTSGGGDSA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016606
• ORF Size: 756 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016606.4
• RefSeq Size: 2203 bp
• RefSeq ORF: 759 bp
• Locus ID: 51308
• UniProt ID: Q9BRK0
• Cytogenetics: 5q31.2
• Protein Families: Druggable Genome, Transmembrane
• MW: 28.3 kDa
• Gene Summary: This gene encodes a member of the receptor expression enhancing protein family. Studies of a related gene in mouse suggest that the encoded protein is found in the cell membrane and enhances the function of sweet taste receptors. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2012]