AP1M1 (NM_032493) Human Tagged ORF Clone

CAT#: RC202366

AP1M1 (Myc-DDK-tagged)-Human adaptor-related protein complex 1, mu 1 subunit (AP1M1), transcript variant 2

Product Images

Circular map for RC202366

• 

  Western blot validation of overexpression lysate (Cat# LY410071) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC202366 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: AP1M1
• Synonyms: AP47; CLAPM2; CLTNM; MU-1A; mu1A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202366 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCGCCAGCGCCGTCTACGTGCTGGACCTGAAGGGCAAGGTGCTCATCTGCCGGAACTACCGTGGCG
  ACGTGGACATGTCAGAGGTGGAGCACTTCATGCCCATCCTGATGGAGAAGGAGGAGGAGGGGATGCTGTC
  GCCCATCCTGGCCCACGGGGGGGTCCGTTTCATGTGGATCAAACACAACAACCTGTATCTGGTTGCCACA
  TCCAAGAAGAACGCGTGCGTGTCGCTGGTCTTTTCTTTCCTCTATAAGGTGGTGCAGGTGTTTTCCGAGT
  ACTTCAAGGAGCTGGAGGAGGAGAGCATCCGGGACAACTTTGTTATCATCTACGAGCTGCTGGACGAGCT
  CATGGACTTCGGCTACCCCCAGACCACCGACAGCAAGATCCTGCAGGAGTACATCACTCAGGAAGGCCAC
  AAGCTGGAAACAGGGGCCCCGCGGCCACCAGCCACCGTCACCAACGCGGTGTCCTGGCGGTCCGAAGGCA
  TCAAGTATCGGAAGAATGAGGTGTTCTTGGACGTCATCGAGTCTGTCAACCTCTTGGTCAGCGCCAACGG
  CAATGTCCTGCGCAGCGAGATCGTGGGCTCCATCAAGATGCGAGTCTTCCTCTCGGGCATGCCCGAGCTG
  CGCCTGGGCCTCAACGACAAGGTCCTCTTTGACAACACGGGCCGCGGCAAAAGCAAATCCGTGGAGCTGG
  AGGATGTGAAGTTCCACCAGTGTGTGCGGCTATCACGCTTCGAGAATGACCGCACCATCTCCTTCATCCC
  ACCCGACGGCGAGTTCGAGCTCATGTCCTACCGTCTCAACACCCACGTCAAGCCTTTGATATGGATCGAG
  TCGGTGATCGAGAAGCACTCCCACAGCCGCATCGAGTACATGATCAAGGCCAAAAGCCAGTTCAAGCGGC
  GGTCAACAGCCAACAACGTGGAGATCCACATTCCCGTGCCCAATGATGCCGACTCACCCAAGTTCAAGAC
  GACGGTGGGGAGCGTTAAGTGGGTCCCCGAGAACAGCGAGATTGTGTGGTCCATCAAGTCCTTCCCGGGC
  GGCAAGGAGTACCTGATGCGGGCCCACTTCGGCCTGCCTAGTGTGGAGGCCGAAGACAAGGAGGGCAAGC
  CCCCGATCAGTGTCAAGTTCGAGATCCCTTACTTCACTACCTCCGGCATCCAGGTGCGCTACCTGAAGAT
  CATTGAGAAGAGTGGGTACCAGGCCCTGCCCTGGGTGCGTTATATCACGCAGAATGGAGATTACCAGCTC
  CGGACCCAG
  
  ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGA
  TTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202366 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSASAVYVLDLKGKVLICRNYRGDVDMSEVEHFMPILMEKEEEGMLSPILAHGGVRFMWIKHNNLYLVAT
  SKKNACVSLVFSFLYKVVQVFSEYFKELEEESIRDNFVIIYELLDELMDFGYPQTTDSKILQEYITQEGH
  KLETGAPRPPATVTNAVSWRSEGIKYRKNEVFLDVIESVNLLVSANGNVLRSEIVGSIKMRVFLSGMPEL
  RLGLNDKVLFDNTGRGKSKSVELEDVKFHQCVRLSRFENDRTISFIPPDGEFELMSYRLNTHVKPLIWIE
  SVIEKHSHSRIEYMIKAKSQFKRRSTANNVEIHIPVPNDADSPKFKTTVGSVKWVPENSEIVWSIKSFPG
  GKEYLMRAHFGLPSVEAEDKEGKPPISVKFEIPYFTTSGIQVRYLKIIEKSGYQALPWVRYITQNGDYQL
  RTQ
  
  TRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-NotI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032493
• ORF Size: 1269 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032493.4
• RefSeq Size: 2373 bp
• RefSeq ORF: 1272 bp
• Locus ID: 8907
• UniProt ID: Q9BXS5
• Cytogenetics: 19p13.11
• Domains: Adap_comp_sub
• Protein Pathways: Lysosome
• MW: 48.6 kDa
• Gene Summary: The protein encoded by this gene is the medium chain of the trans-Golgi network clathrin-associated protein complex AP-1. The other components of this complex are beta-prime-adaptin, gamma-adaptin, and the small chain AP1S1. This complex is located at the Golgi vesicle and links clathrin to receptors in coated vesicles. These vesicles are involved in endocytosis and Golgi processing. Alternatively spliced transcript variants encoding distinct protein isoforms have been found for this gene. [provided by RefSeq, Jul 2008]