GAPDH (NM_002046) Human Tagged ORF Clone
GAPDH (Myc-DDK-tagged)-Human glyceraldehyde-3-phosphate dehydrogenase (GAPDH)
|Product Name||GAPDH (NM_002046) Human Tagged ORF Clone|
|Type||Human Tagged ORF Clone|
|Synonyms||G3PD; GAPD; HEL-S-162eP|
|E. coli Selection||Kanamycin (25 ug/mL)|
>RC202309 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)
>RC202309 protein sequence
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||1005 bp|
|OTI Disclaimer||Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at email@example.com or by calling 301.340.3188 option 3 for pricing and delivery.
The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq||NM_002046.1, NM_002046.2, NM_002046.3, NM_002046.4, NM_002046.5, NP_002037.2|
|Protein Families||ES Cell Differentiation/IPS|
|Protein Pathways||Alzheimer's disease, Glycolysis / Gluconeogenesis, Metabolic pathways|
|Gene Summary||This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014]|
|The use of this cDNA Clones has been cited in the following citations:|
GAPDH controls extracellular vesicle biogenesis and enhances therapeutic potential of EVs in silencing the Huntingtin gene in mice via siRNA delivery
,Dar, GH;Mendes, CC;Kuan, WL;Conceicao, M;,
Malonylation of GAPDH is an inflammatory signal in macrophages
,Galván-Peña, S;Carroll, RG;Newman, C;Hinchy, EC;Palsson-McDermott, E;Robinson, EK;Covarrubias, S;Nadin, A;James, AM;Haneklaus, M;Carpenter, S;Kelly, VP;Murphy, MP;Modis, LK;O'Neill, LA;,
Nat Commun 2019
,PubMed ID 30659183
GAPDH with NAD+-binding site mutation competitively inhibits the wild-type and affects glucose metabolism in cancer
,Kunjithapatham, R;Ganapathy-Kanniappan, S;,
Biochim. Biophys. Acta 2018
,PubMed ID 30077773
Protein CoAlation: A Redox-Regulated Protein Modification by Coenzyme Ain Mammalian Cells
,Tsuchiya, Y;Peak-Chew, S;Newell, C;Miller-Aidoo, S;Mangal, S;Zhyvoloup, A;Baković, J;Malanchuk, O;Pereira, G;Kotiadis, V;Szabadkai, G;Duchen, M;Campbell, M;Rodriguez Cuenca, S;Vidal-Puig, A;James, A;Murphy, MP;Filonenko, V;Skehel, M;Gout, I;,
Biochem. J 2017
,PubMed ID 28341808
Overexpression of glyceraldehyde 3-phosphate dehydrogenase prevents neurovascular degeneration after retinal injury
,Cai, R;Xue, W;Liu, S;Petersen, RB;Huang, K;Zheng, L;,
FASEB J. Mar 2015
,PubMed ID 25805836
Glyceraldehyde-3-phosphate dehydrogenase acts as a mitochondrial trans-s-nitrosylase in the heart
,Kohr, MJ;Murphy, E;Steenbergen, C;,
PLoS ONE Oct 2014
,PubMed ID 25347796
|cDNA Clone Resources|
|RC202309L4||Lenti ORF clone of Human glyceraldehyde-3-phosphate dehydrogenase (GAPDH), mGFP tagged||
|RC202309L3||Lenti ORF clone of Human glyceraldehyde-3-phosphate dehydrogenase (GAPDH), Myc-DDK-tagged||
|RC202309L2||Lenti ORF clone of Human glyceraldehyde-3-phosphate dehydrogenase (GAPDH), mGFP tagged||
|RC202309L1||Lenti ORF clone of Human glyceraldehyde-3-phosphate dehydrogenase (GAPDH), Myc-DDK-tagged||
|RG202309||GAPDH (GFP-tagged) - Human glyceraldehyde-3-phosphate dehydrogenase (GAPDH)||
|SC118869||GAPDH (untagged)-Human glyceraldehyde-3-phosphate dehydrogenase (GAPDH)||