PRKACB (NM_207578) Human Tagged ORF Clone
PRKACB (Myc-DDK-tagged)-Human protein kinase, cAMP-dependent, catalytic, beta (PRKACB), transcript variant 3
|Product Name||PRKACB (NM_207578) Human Tagged ORF Clone|
|Type||Human Tagged ORF Clone|
|Synonyms||PKA C-beta; PKACB|
|E. coli Selection||Kanamycin (25 ug/mL)|
>RC202131 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)
>RC202131 protein sequence
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||771 bp|
|OTI Disclaimer||The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info|
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq||NM_207578.1, NM_207578.2, NM_207578.3, NP_997461.1|
|RefSeq Size||2119 bp|
|RefSeq ORF||774 bp|
|Protein Families||Druggable Genome, Protein Kinase|
|Protein Pathways||Apoptosis, Calcium signaling pathway, Chemokine signaling pathway, Dilated cardiomyopathy, Gap junction, GnRH signaling pathway, Hedgehog signaling pathway, Insulin signaling pathway, Long-term potentiation, MAPK signaling pathway, Melanogenesis, Olfactory transduction, Oocyte meiosis, Prion diseases, Progesterone-mediated oocyte maturation, Taste transduction, Vascular smooth muscle contraction, Vibrio cholerae infection, Wnt signaling pathway|
|Gene Summary||The protein encoded by this gene is a member of the serine/threonine protein kinase family. The encoded protein is a catalytic subunit of cAMP (cyclic AMP)-dependent protein kinase, which mediates signalling though cAMP. cAMP signaling is important to a number of processes, including cell proliferaton and differentiation. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2014]|
|The use of this cDNA Clones has been cited in the following citations:|
MicroRNA-200a-3p Mediates Neuroprotection in Alzheimer-Related Deficits and Attenuates Amyloid-Beta Overproduction and Tau Hyperphosphorylation via Coregulating BACE1 and PRKACB
,Wang, L;Liu, J;Wang, Q;Jiang, H;Zeng, L;Li, Z;Liu, R;,
Front Pharmacol 2019
,PubMed ID 31379578
|cDNA Clone Resources|
|SC322379||PRKACB (untagged)-Human protein kinase, cAMP-dependent, catalytic, beta (PRKACB), transcript variant 3||
|RG202131||PRKACB (GFP-tagged) - Human protein kinase, cAMP-dependent, catalytic, beta (PRKACB), transcript variant 3||
|RC202131L1||Lenti ORF clone of Human protein kinase, cAMP-dependent, catalytic, beta (PRKACB), transcript variant 3, Myc-DDK-tagged||
|RC202131L2||Lenti ORF clone of Human protein kinase, cAMP-dependent, catalytic, beta (PRKACB), transcript variant 3, mGFP tagged||
|RC202131L3||Lenti ORF clone of Human protein kinase, cAMP-dependent, catalytic, beta (PRKACB), transcript variant 3, Myc-DDK-tagged||
|RC202131L4||Lenti ORF clone of Human protein kinase, cAMP-dependent, catalytic, beta (PRKACB), transcript variant 3, mGFP tagged||