TRIM21 (NM_003141) Human Tagged ORF Clone

CAT#: RC202088

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  • TrueORF®

TRIM21 (Myc-DDK-tagged)-Human tripartite motif containing 21 (TRIM21)



  "NM_003141" in other vectors (6)

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 477.00


Availability*
In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol TRIM21
Synonyms RNF81; Ro/SSA; RO52; SSA; SSA1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC202088 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTTCAGCAGCACGCTTGACAATGATGTGGGAGGAGGTCACATGCCCTATCTGCCTGGACCCCTTCG
TGGAGCCTGTGAGCATCGAGTGTGGCCACAGCTTCTGCCAGGAATGCATCTCTCAGGTTGGGAAAGGTGG
GGGCAGCGTCTGTCCTGTGTGCCGGCAGCGCTTTCTGCTCAAGAATCTCCGGCCCAATCGACAGCTAGCC
AACATGGTGAACAACCTTAAAGAAATCAGCCAGGAGGCCAGAGAGGGCACACAGGGGGAACGGTGTGCAG
TGCATGGAGAGAGACTTCACCTGTTCTGTGAGAAAGATGGGAAGGCCCTTTGCTGGGTATGTGCCCAGTC
TCGGAAACACCGTGACCACGCCATGGTCCCTCTTGAGGAGGCTGCACAGGAGTACCAGGAGAAGCTCCAG
GTGGCATTAGGGGAACTGAGAAGAAAGCAGGAGTTGGCTGAGAAGTTGGAAGTGGAAATTGCAATAAAGA
GAGCAGACTGGAAGAAAACAGTGGAAACACAGAAATCTAGGATTCACGCAGAGTTTGTGCAGCAAAAAAA
CTTCCTGGTTGAAGAAGAACAGAGGCAGCTGCAGGAGCTGGAGAAGGATGAGAGGGAGCAGCTGAGAATC
CTGGGGGAGAAAGAGGCCAAGCTGGCCCAGCAGAGCCAGGCCCTACAGGAGCTCATCTCAGAGCTAGATC
GAAGGTGCCACAGCTCAGCACTGGAACTGCTGCAGGAGGTGATAATTGTCCTGGAAAGGAGTGAGTCCTG
GAACCTGAAGGACCTGGATATTACCTCTCCAGAACTCAGGAGTGTGTGCCATGTGCCAGGGCTGAAGAAG
ATGCTGAGGACATGTGCAGTCCACATCACTCTGGATCCAGACACAGCCAATCCGTGGCTGATACTTTCAG
AAGATCGGAGACAAGTGAGGCTTGGAGACACCCAGCAGAGCATACCTGGAAATGAAGAGAGATTTGATAG
TTATCCTATGGTCCTGGGTGCCCAGCACTTTCACTCTGGAAAACATTACTGGGAGGTAGATGTGACAGGA
AAGGAGGCCTGGGACCTGGGTGTCTGCAGAGACTCTGTGCGCAGGAAGGGGCACTTTTTGCTTAGTTCCA
AGAGTGGCTTCTGGACAATTTGGTTGTGGAACAAACAAAAATATGAGGCTGGCACCTACCCCCAGACTCC
CCTCCACCTTCAGGTGCCTCCATGCCAAGTTGGGATTTTCCTGGACTATGAGGCTGGCATGGTCTCCTTC
TACAACATCACTGACCATGGCTCCCTCATCTACTCCTTCTCTGAATGTGCCTTTACAGGACCTCTGCGGC
CCTTCTTCAGTCCTGGTTTCAATGATGGAGGAAAAAACACAGCCCCTCTAACCCTCTGTCCACTGAATAT
TGGATCACAAGGATCCACTGACTAT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC202088 protein sequence
Red=Cloning site Green=Tags(s)

MASAARLTMMWEEVTCPICLDPFVEPVSIECGHSFCQECISQVGKGGGSVCPVCRQRFLLKNLRPNRQLA
NMVNNLKEISQEAREGTQGERCAVHGERLHLFCEKDGKALCWVCAQSRKHRDHAMVPLEEAAQEYQEKLQ
VALGELRRKQELAEKLEVEIAIKRADWKKTVETQKSRIHAEFVQQKNFLVEEEQRQLQELEKDEREQLRI
LGEKEAKLAQQSQALQELISELDRRCHSSALELLQEVIIVLERSESWNLKDLDITSPELRSVCHVPGLKK
MLRTCAVHITLDPDTANPWLILSEDRRQVRLGDTQQSIPGNEERFDSYPMVLGAQHFHSGKHYWEVDVTG
KEAWDLGVCRDSVRRKGHFLLSSKSGFWTIWLWNKQKYEAGTYPQTPLHLQVPPCQVGIFLDYEAGMVSF
YNITDHGSLIYSFSECAFTGPLRPFFSPGFNDGGKNTAPLTLCPLNIGSQGSTDY

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_003141
ORF Size 1425 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_003141.4
RefSeq Size 1946 bp
RefSeq ORF 1428 bp
Locus ID 6737
UniProt ID P19474
Cytogenetics 11p15.4
Domains zf-B_box, RING, SPRY, PRY
Protein Families Druggable Genome
Protein Pathways Systemic lupus erythematosus
MW 54.2 kDa
Gene Summary This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The encoded protein is part of the RoSSA ribonucleoprotein, which includes a single polypeptide and one of four small RNA molecules. The RoSSA particle localizes to both the cytoplasm and the nucleus. RoSSA interacts with autoantigens in patients with Sjogren syndrome and systemic lupus erythematosus. Alternatively spliced transcript variants for this gene have been described but the full-length nature of only one has been determined. [provided by RefSeq, Jul 2008]

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