TRIM21 (NM_003141) Human Untagged Clone

SKU SC118185

TRIM21 (untagged)-Human tripartite motif containing 21 (TRIM21)

Product Data

• Type: Human Untagged Clone
• Target Symbol: TRIM21
• Synonyms: RNF81; Ro/SSA; RO52; SSA; SSA1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_003141, the custom clone sequence may differ by one or more nucleotides; ; ; ATGGCTTCAGCAGCACGCTTGACAATGATGTGGGAGGAGGTCACATGCCCTATCTGCCTGGACCCCTTCG; TGGAGCCTGTGAGCATCGAGTGTGGCCACAGCTTCTGCCAGGAATGCATCTCTCAGGTTGGGAAAGGTGG; GGGCAGCGTCTGTCCTGTGTGCCGGCAGCGCTTTCTGCTCAAGAATCTCCGGCCCAATCGACAGCTAGCC; AACATGGTGAACAACCTTAAAGAAATCAGCCAGGAGGCCAGAGAGGGCACACAGGGGGAACGGTGTGCAG; TGCATGGAGAGAGACTTCACCTGTTCTGTGAGAAAGATGGGAAGGCCCTTTGCTGGGTATGTGCCCAGTC; TCGGAAACACCGTGACCACGCCATGGTCCCTCTTGAGGAGGCTGCACAGGAGTACCAGGAGAAGCTCCAG; GTGGCATTAGGGGAACTGAGAAGAAAGCAGGAGTTGGCTGAGAAGTTGGAAGTGGAAATTGCAATAAAGA; GAGCAGACTGGAAGAAAACAGTGGAAACACAGAAATCTAGGATTCACGCAGAGTTTGTGCAGCAAAAAAA; CTTCCTGGTTGAAGAAGAACAGAGGCAGCTGCAGGAGCTGGAGAAGGATGAGAGGGAGCAGCTGAGAATC; CTGGGGGAGAAAGAGGCCAAGCTGGCCCAGCAGAGCCAGGCCCTACAGGAGCTCATCTCAGAGCTAGATC; GAAGGTGCCACAGCTCAGCACTGGAACTGCTGCAGGAGGTGATAATTGTCCTGGAAAGGAGTGAGTCCTG; GAACCTGAAGGACCTGGATATTACCTCTCCAGAACTCAGGAGTGTGTGCCATGTGCCAGGGCTGAAGAAG; ATGCTGAGGACATGTGCAGTCCACATCACTCTGGATCCAGACACAGCCAATCCGTGGCTGATACTTTCAG; AAGATCGGAGACAAGTGAGGCTTGGAGACACCCAGCAGAGCATACCTGGAAATGAAGAGAGATTTGATAG; TTATCCTATGGTCCTGGGTGCCCAGCACTTTCACTCTGGAAAACATTACTGGGAGGTAGATGTGACAGGA; AAGGAGGCCTGGGACCTGGGTGTCTGCAGAGACTCTGTGCGCAGGAAGGGGCACTTTTTGCTTAGTTCCA; AGAGTGGCTTCTGGACAATTTGGTTGTGGAACAAACAAAAATATGAGGCTGGCACCTACCCCCAGACTCC; CCTCCACCTTCAGGTGCCTCCATGCCAAGTTGGGATTTTCCTGGACTATGAGGCTGGCATGGTCTCCTTC; TACAACATCACTGACCATGGCTCCCTCATCTACTCCTTCTCTGAATGTGCCTTTACAGGACCTCTGCGGC; CCTTCTTCAGTCCTGGTTTCAATGATGGAGGAAAAAACACAGCCCCTCTAACCCTCTGTCCACTGAATAT; TGGATCACAAGGATCCACTGACTATTGA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003141 unedited; CGCCCCCCAGAANNAAACACACCACCCNTCCGCGGTTCATAATTGTATAAGACTCACTAT; AGGCGGCCGCGAAATTCGCACGAGGTCCCCTGTAAAGCCAAACCCCCTAAAGTCTCCACA; CTGCTGTTTAACGGCACACTTGACAATGGCTTCAGCAGCACGCTTGACAATGATGTGGGA; GGAGGTCACATGCCCTATCTGCCTGGACCCCTTCGTGGAGCCTGTGAGCATCGAGTGTGG; CCACAGCTTCTGCCAGGAATGCATCTCTCAGGTTGGGAAAGGTGGGGGCAGCGTCTGTCC; TGTGTGCCGGCAGCGCTTTCTGCTCAAGAATCTCCGGCCCAATCGACAGCTAGCCAACAT; GGTGAACAACCTTAAAGAAATCAGCCAGGAGGCCAGAGAGGGCACACAGGGGGAACGGTG; TGCAGTGCATGGAGAGAGACTTCACCTGTTCTGTGAGAAAGATGGGAAGGCCCTTTGCTG; GGTATGTGCCCAGTCTCGGAAACACCGTGACCACGCCATGGTCCCTCTTGAGGAGGCTGC; ACAGGAGTACCAGGAGAAGCTCCAGGTGGCATTAGGGGAACTGAGAAGAAAGCAGGAGTT; GGCTGAGAAGTTGGAAGTGGAAATTGCAATAAAGAGAGCAGACTGGAAGAAAACAGTGGA; AACACAGAAATCTAGGATTCACGCAGAGTTTGTGCAGCANAAAAACTTCCTGTTTGAAGA; AGAACCAGAGCAGCTGCANGAACTGGAGAAGGATGACAGGGAGCACCTGAGAATCCTGCG; GGAGAAAGAGCCCAGCTGGCCCAGCAGAGCCAGCCCCTACAGGAGCTCATCTCAGAGCTA; GATCCGAAGTGCCACAGCTCAACACTGGAACTGCTGCAGGAGTGATTATTGTCCTGGAAG; GAGTGGTCCTGCAAC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003141 unedited; GGGAAACTTAGGAACCGCGGCCGCAATCTANGATCGAGTTTTTTTTTTTTTTTTTTTTTT; TTTTTTTTTTTTTAAATTTTATTTTTTATTTTTAGGAAGCCCTTTTGCAAACCATACAAG; ATATAATAGAGATCTCACCAAAGACGACATCCTAGAGATGGAGAGGAGAAAAAAAAAACT; TAAGTCCCATAAAGAAGGCAGAAACATGTTTTTGGTTGATCAAGATGAGTTTGGGCCAAA; TATAAGGAGGCTGCTTCACTGGAGGGAATCACAAAGCCATCTGGGGCAGGAATGTTGATG; GTGAAGTGACTTCCCTGCTAAAGCTCGCTTGCTGGGATCCGGATGCCTCTGCAGAGACAA; AGGTGGTTCAGAGTTCATGGGGAAAAGAGGCAGGGTTTGTGGCTGAGAAGCGGTGCCAGT; GGGGAGAGTGGCAGTGTCCAGAGAAAGCCATCAATAGTCAGTGGATCCTTGTGATCCAAT; ATTCAGTGGACAGAGGGTTAGAGGGGCTGTGTTTTTTCCTCCATCATTGAAACCAGGACT; GAAGAAGGGCCGCAGAGGTCCTGTAAAGGCACATTCAGAGAAGGAGTAGATGAGGGAGCC; ATGGTCAGTGATGTTGTAGAAGGAGACCATGCCAGCCTCATAGTCCAGGGAAATCCCAAC; CTGGCCGGGGAGGCCCCGGAGGTGGGGGGGAATCCGGGGGTAAGTGCACNCCCCCAAGTT; TTGTGGGGCCCACAGCCAANNTGCCACCAACCACCCCTGGACAACAGCAAAGGGGAGCNN; NNNGCGGCACTGAGGCCGGNNAAACACTGAGGNNNAAGGGGCGCTTTGCCGGTGNNAGCG; AGGTGCGGGGNNNNNTAGCACAGAGGCAAATGGGCGGGNNCTCAGCGGACCAGGGAAAAC; GAGAA;
• Restriction Sites: ECoRI-NOT
• ACCN: NM_003141
• Insert Size: 2000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.; ; The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_003141.3, NP_003132.2
• RefSeq Size: 1946 bp
• RefSeq ORF: 1428 bp
• Locus ID: 6737
• UniProt ID: P19474
• Cytogenetics: 11p15.4
• Domains: PRY, RING, SPRY, zf-B_box
• Protein Families: Druggable Genome
• Protein Pathways: Systemic lupus erythematosus
• Summary: This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The encoded protein is part of the RoSSA ribonucleoprotein, which includes a single polypeptide and one of four small RNA molecules. The RoSSA particle localizes to both the cytoplasm and the nucleus. RoSSA interacts with autoantigens in patients with Sjogren syndrome and systemic lupus erythematosus. Alternatively spliced transcript variants for this gene have been described but the full-length nature of only one has been determined. [provided by RefSeq, Jul 2008]