IL8 (CXCL8) (NM_000584) Human Tagged ORF Clone
IL8 (Myc-DDK-tagged)-Human interleukin 8 (IL8)
|Product Name||IL8 (CXCL8) (NM_000584) Human Tagged ORF Clone|
|Type||Human Tagged ORF Clone|
|Synonyms||GCP-1; GCP1; IL8; LECT; LUCT; LYNAP; MDNCF; MONAP; NAF; NAP-1; NAP1|
|E. coli Selection||Kanamycin (25 ug/mL)|
>RC202075 representing NM_000584
Red=Cloning site Blue=ORF Green=Tags(s)
>RC202075 representing NM_000584
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||297 bp|
|OTI Disclaimer||Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at firstname.lastname@example.org or by calling 301.340.3188 option 3 for pricing and delivery.
The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq||NM_000584.1, NM_000584.2, NM_000584.3, NM_000584.4, NP_000575.1|
|RefSeq Size||1666 bp|
|RefSeq ORF||300 bp|
|Protein Families||Druggable Genome, Secreted Protein, Transmembrane|
|Protein Pathways||Bladder cancer, Chemokine signaling pathway, Cytokine-cytokine receptor interaction, Epithelial cell signaling in Helicobacter pylori infection, NOD-like receptor signaling pathway, Pathways in cancer, RIG-I-like receptor signaling pathway, Toll-like receptor signaling pathway|
|Gene Summary||The protein encoded by this gene is a member of the CXC chemokine family and is a major mediator of the inflammatory response. The encoded protein is commonly referred to as interleukin-8 (IL-8). IL-8 is secreted by mononuclear macrophages, neutrophils, eosinophils, T lymphocytes, epithelial cells, and fibroblasts. It functions as a chemotactic factor by guiding the neutrophils to the site of infection. Bacterial and viral products rapidly induce IL-8 expression. IL-8 also participates with other cytokines in the proinflammatory signaling cascade and plays a role in systemic inflammatory response syndrome (SIRS). This gene is believed to play a role in the pathogenesis of the lower respiratory tract infection bronchiolitis, a common respiratory tract disease caused by the respiratory syncytial virus (RSV). The overproduction of this proinflammatory protein is thought to cause the lung inflammation associated with csytic fibrosis. This proinflammatory protein is also suspected of playing a role in coronary artery disease and endothelial dysfunction. This protein is also secreted by tumor cells and promotes tumor migration, invasion, angiogenesis and metastasis. This chemokine is also a potent angiogenic factor. The binding of IL-8 to one of its receptors (IL-8RB/CXCR2) increases the permeability of blood vessels and increasing levels of IL-8 are positively correlated with increased severity of multiple disease outcomes (eg, sepsis). This gene and other members of the CXC chemokine gene family form a gene cluster in a region of chromosome 4q. [provided by RefSeq, May 2020]|
|The use of this cDNA Clones has been cited in the following citations:|
A Simple and Efficient System for Producing Recombinant Human CXCL8 in Escherichia coli
,Huang, C;Zhong, S;Park, H;Jeong, JH;Luo, JL;,
J. Interferon Cytokine Res. 2020
,PubMed ID 32780615
Interleukin 8 is a biomarker of telomerase inhibition in cancer cells
,Solomon, P;Dong, Y;Dogra, S;Gupta, R;,
BMC Cancer 2018
,PubMed ID 29986697
Serine hydroxymethyl transferase 1 stimulates pro-oncogenic cytokine expression through sialic acid to promote ovarian cancer tumor growth and progression
,Gupta, R;Yang, Q;Dogra, SK;Wajapeyee, N;,
,PubMed ID 28288142
The combined effects of physicochemical properties ofsize-fractionated ambient particulate matter on in vitrotoxicity in human A549 lung epithelial cells
,Akhtar, US;Rastogi, N;McWhinney, RD;Urch, B;,
Toxicology Reports May 2014
|cDNA Clone Resources|
|SC119807||IL8 (untagged)-Human interleukin 8 (IL8)||
|RG202075||IL8 (GFP-tagged) - Human interleukin 8 (IL8)||
|RC202075L1||Lenti ORF clone of Human interleukin 8 (IL8), Myc-DDK-tagged||
|RC202075L2||Lenti ORF clone of Human interleukin 8 (IL8), mGFP tagged||
|RC202075L3||Lenti ORF clone of Human interleukin 8 (IL8), Myc-DDK-tagged||
|RC202075L4||Lenti ORF clone of Human interleukin 8 (IL8), mGFP tagged||