PAM (NM_138821) Human Tagged ORF Clone

CAT#: RC202074

PAM (Myc-DDK-tagged)-Human peptidylglycine alpha-amidating monooxygenase (PAM), transcript variant 3

Product Images

Western blot validation of overexpression lysate (Cat# LY424457) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC212581 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified PAM protein (Cat# TP302074). The protein was produced from HEK293T cells transfected with PAM cDNA clone (Cat# RC202074) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PAM
• Synonyms: PAL; PHM
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202074 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGGCCGCGTCCCTAGCCTGCTAGTTCTCCTTGTTTTTCCAAGCAGCTGTTTGGCTTTCCGAAGCC
  CACTTTCTGTCTTTAAGAGGTTTAAAGAAACTACCAGACCATTTTCCAATGAATGTCTTGGTACCACCAG
  ACCCGTAGTTCCTATTGATTCATCAGATTTTGCATTGGATATTCGCATGCCTGGGGTTACACCTAAACAG
  TCCGATACATACTTCTGCATGTCTATGCGAATACCAGTGGATGAGGAAGCCTTCGTGATTGACTTCAAGC
  CTCGAGCCAGCATGGATACTGTCCATCACATGTTACTTTTTGGATGCAATATGCCTTCATCCACTGGAAG
  TTACTGGTTTTGTGATGAAGGAACCTGTACAGATAAAGCCAATATTCTGTATGCCTGGGCGAGAAATGCT
  CCCCCTACCCGGCTCCCCAAAGGTGTTGGATTCAGAGTTGGAGGAGAGACTGGAAGTAAATACTTTGTAC
  TACAGGTACACTATGGGGATATTAGTGCTTTTAGAGATAATAACAAGGACTGTTCTGGTGTGTCCTTACA
  CCTCACACGTCTGCCACAGCCTTTAATTGCTGGCATGTACCTTATGATGTCTGTTGACACTGTTATCCCA
  GCAGGAGAAAAAGTGGTGAATTCTGACATTTCATGCCATTATAAAAATTATCCAATGCATGTCTTTGCCT
  ATAGAGTTCACACTCACCATTTAGGTAAGGTAGTAAGTGGATACAGAGTAAGAAATGGACAGTGGACACT
  GATTGGACGGCAGAGCCCTCAGCTGCCACAGGCTTTCTACCCTGTGGGGCATCCAGTTGATGTAAGTTTT
  GGTGACCTACTGGCTGCAAGATGTGTATTCACTGGTGAAGGAAGGACAGAAGCCACACACATTGGTGGCA
  CGTCTAGTGATGAAATGTGCAACTTATACATTATGTATTACATGGAAGCCAAGCATGCAGTTTCTTTCAT
  GACCTGTACCCAGAATGTAGCTCCAGATATGTTCAGAACCATACCACCAGAGGCCAACATTCCAATTCCC
  GTGAAGTCTGATATGGTTATGATGCATGAACATCATAAAGAAACAGAATATAAAGATAAGATTCCTTTAC
  TACAGCAGCCAAAACGAGAAGAAGAAGAAGTGTTAGACCAGGATTTCCACATGGAAGAGGCACTGGATTG
  GCCTGGAGTATACTTGTTACCAGGCCAGGTTTCTGGGGTGGCTCTAGACCCTAAGAATAACCTGGTGATT
  TTCCACAGAGGTGACCATGTCTGGGATGGAAACTCGTTTGACAGCAAGTTTGTTTACCAGCAAATAGGAC
  TCGGACCAATTGAAGAAGACACTATTCTTGTCATAGATCCAAATAATGCTGCAGTACTCCAGTCCAGTGG
  AAAAAATCTGTTTTACTTGCCACATGGCTTGAGTATAGATAAAGATGGGAATTATTGGGTCACAGACGTG
  GCTCTCCATCAGGTGTTCAAACTGGATCCAAACAATAAAGAAGGCCCTGTATTAATCCTGGGAAGGAGCA
  TGCAACCAGGCAGTGACCAGAATCACTTCTGTCAACCCACTGATGTGGCTGTGGATCCAGGCACTGGAGC
  CATTTATGTATCAGATGGTTACTGCAACAGCAGGATTGTGCAGTTTTCACCAAGTGGAAAGTTCATCACA
  CAGTGGGGAGAAGAGTCTTCAGGGAGCAGTCCTCTGCCAGGCCAGTTCACTGTTCCTCACAGCTTGGCTC
  TTGTGCCTCTTTTGGGCCAATTATGTGTGGCAGACCGGGAAAATGGTCGGATCCAGTGTTTTAAAACTGA
  CACCAAAGAATTTGTGAGAGAGATTAAGCATTCATCATTTGGAAGAAATGTATTTGCAATTTCATATATA
  CCAGGCTTGCTCTTTGCAGTGAATGGGAAGCCTCATTTTGGGGACCAAGAACCTGTACAAGGATTTGTGA
  TGAACTTTTCCAATGGGGAAATTATCGACATCTTCAAGCCAGTGCGCAAGCACTTTGATATGCCTCATGA
  TATTGTTGCATCTGAAGATGGGACTGTGTACATTGGAGATGCTCATACCAACACCGTGTGGAAGTTCACC
  TTGACTGAGAAATTGGAACATCGATCAGTTAAAAAGGCTGGCATTGAGGTCCAGGAAATCAAAGAAGCCG
  AGGCAGTTGTTGAAACCAAAATGGAGAACAAACCCACCTCCTCAGAATTGCAGAAGATGCAAGAGAAACA
  GAAACTGATCAAAGAGCCAGGCTCGGGAGTGCCTGTTGTTCTCATTACAACCCTTCTGGTTATTCCGGTG
  GTTGTCCTGCTGGCCATTGCCATATTTATTCGGTGGAAAAAATCAAGGGCCTTTGGAGATTCTGAACACA
  AACTCGAGACGAGTTCAGGAAGAGTACTGGGAAGATTTAGAGGAAAGGGAAGTGGAGGCTTAAACCTTGG
  TAATTTCTTTGCAAGCCGTAAGGGCTACAGTCGAAAAGGGTTTGACCGGCTTAGCACTGAGGGCAGTGAC
  CAAGAGAAAGAGGATGATGGAAGTGAATCAGAAGAGGAGTATTCAGCACCTCTGCCTGCGCTCGCACCTT
  CCTCCTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202074 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAGRVPSLLVLLVFPSSCLAFRSPLSVFKRFKETTRPFSNECLGTTRPVVPIDSSDFALDIRMPGVTPKQ
  SDTYFCMSMRIPVDEEAFVIDFKPRASMDTVHHMLLFGCNMPSSTGSYWFCDEGTCTDKANILYAWARNA
  PPTRLPKGVGFRVGGETGSKYFVLQVHYGDISAFRDNNKDCSGVSLHLTRLPQPLIAGMYLMMSVDTVIP
  AGEKVVNSDISCHYKNYPMHVFAYRVHTHHLGKVVSGYRVRNGQWTLIGRQSPQLPQAFYPVGHPVDVSF
  GDLLAARCVFTGEGRTEATHIGGTSSDEMCNLYIMYYMEAKHAVSFMTCTQNVAPDMFRTIPPEANIPIP
  VKSDMVMMHEHHKETEYKDKIPLLQQPKREEEEVLDQDFHMEEALDWPGVYLLPGQVSGVALDPKNNLVI
  FHRGDHVWDGNSFDSKFVYQQIGLGPIEEDTILVIDPNNAAVLQSSGKNLFYLPHGLSIDKDGNYWVTDV
  ALHQVFKLDPNNKEGPVLILGRSMQPGSDQNHFCQPTDVAVDPGTGAIYVSDGYCNSRIVQFSPSGKFIT
  QWGEESSGSSPLPGQFTVPHSLALVPLLGQLCVADRENGRIQCFKTDTKEFVREIKHSSFGRNVFAISYI
  PGLLFAVNGKPHFGDQEPVQGFVMNFSNGEIIDIFKPVRKHFDMPHDIVASEDGTVYIGDAHTNTVWKFT
  LTEKLEHRSVKKAGIEVQEIKEAEAVVETKMENKPTSSELQKMQEKQKLIKEPGSGVPVVLITTLLVIPV
  VVLLAIAIFIRWKKSRAFGDSEHKLETSSGRVLGRFRGKGSGGLNLGNFFASRKGYSRKGFDRLSTEGSD
  QEKEDDGSESEEEYSAPLPALAPSSS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_138821
• ORF Size: 2598 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_138821.2
• RefSeq Size: 5035 bp
• RefSeq ORF: 2601 bp
• Locus ID: 5066
• UniProt ID: P19021
• Cytogenetics: 5q21.1
• Protein Families: Druggable Genome, Transmembrane
• MW: 96.3 kDa
• Gene Summary: This gene encodes a multifunctional protein. The encoded preproprotein is proteolytically processed to generate the mature enzyme. This enzyme includes two domains with distinct catalytic activities, a peptidylglycine alpha-hydroxylating monooxygenase (PHM) domain and a peptidyl-alpha-hydroxyglycine alpha-amidating lyase (PAL) domain. These catalytic domains work sequentially to catalyze the conversion of neuroendocrine peptides to active alpha-amidated products. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Jan 2016]